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Upregulation of Epstein-Barr virus-encoded latent membrane protein by human herpesvirus 6 superinfection of EBV-carrying Burkitt lymphoma cells.
J Med Virol. 1998 Jul; 55(3):219-26.JM

Abstract

The effect of HHV-6 strain A infection on the expression of Epstein-Barr virus- (EBV-) encoded growth transformation-associated genes in two EBV-positive Burkitt lymphoma cell lines, Akata and P3HR-3, was investigated. The results indicate that HHV-6A upregulates the expression of the latent membrane protein LMP-1 in both cell lines. Expression of EBNA-2 was also upregulated in Akata cells following HHV-6A infection. Transfection of reporter constructs carrying the LMP-1 regulatory sequences (LRS; -634/+40) or its 5' deleted derivatives in Akata and in a T-lymphoblastoid cell line, J-Jhan, confirmed the presence of positive and negative regulatory elements responsive to HHV-6A infection in LMP-1 regulatory sequence (LRS). The majority of LRS constructs were under the influence of dominant negative factors. HHV-6A was able to override the effect of such factors acting on reporter plasmids containing the -634/-54, -324/-54, -214/-54, and -106/-54 parts of LRS. The plasmid that carried only the -54/+40 LRS region was constitutively active in both Akata and J-Jhan cells; in Akata, its activity was influenced by HHV-6A. The finding that HHV-6A infection may activate LMP-1 and EBNA-2 expression, which is essential for the immortalization of B-lymphocytes by EBV, shows a novel aspect of the interaction between these two herpesviruses.

Authors+Show Affiliations

Istituto Neurologico Mediterraneo Neuromed, Pozzilli, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9624610

Citation

Cuomo, L, et al. "Upregulation of Epstein-Barr Virus-encoded Latent Membrane Protein By Human Herpesvirus 6 Superinfection of EBV-carrying Burkitt Lymphoma Cells." Journal of Medical Virology, vol. 55, no. 3, 1998, pp. 219-26.
Cuomo L, Trivedi P, de Grazia U, et al. Upregulation of Epstein-Barr virus-encoded latent membrane protein by human herpesvirus 6 superinfection of EBV-carrying Burkitt lymphoma cells. J Med Virol. 1998;55(3):219-26.
Cuomo, L., Trivedi, P., de Grazia, U., Calogero, A., D'Onofrio, M., Yang, W., Frati, L., Faggioni, A., Rymo, L., & Ragona, G. (1998). Upregulation of Epstein-Barr virus-encoded latent membrane protein by human herpesvirus 6 superinfection of EBV-carrying Burkitt lymphoma cells. Journal of Medical Virology, 55(3), 219-26.
Cuomo L, et al. Upregulation of Epstein-Barr Virus-encoded Latent Membrane Protein By Human Herpesvirus 6 Superinfection of EBV-carrying Burkitt Lymphoma Cells. J Med Virol. 1998;55(3):219-26. PubMed PMID: 9624610.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulation of Epstein-Barr virus-encoded latent membrane protein by human herpesvirus 6 superinfection of EBV-carrying Burkitt lymphoma cells. AU - Cuomo,L, AU - Trivedi,P, AU - de Grazia,U, AU - Calogero,A, AU - D'Onofrio,M, AU - Yang,W, AU - Frati,L, AU - Faggioni,A, AU - Rymo,L, AU - Ragona,G, PY - 1998/6/13/pubmed PY - 2000/6/20/medline PY - 1998/6/13/entrez SP - 219 EP - 26 JF - Journal of medical virology JO - J Med Virol VL - 55 IS - 3 N2 - The effect of HHV-6 strain A infection on the expression of Epstein-Barr virus- (EBV-) encoded growth transformation-associated genes in two EBV-positive Burkitt lymphoma cell lines, Akata and P3HR-3, was investigated. The results indicate that HHV-6A upregulates the expression of the latent membrane protein LMP-1 in both cell lines. Expression of EBNA-2 was also upregulated in Akata cells following HHV-6A infection. Transfection of reporter constructs carrying the LMP-1 regulatory sequences (LRS; -634/+40) or its 5' deleted derivatives in Akata and in a T-lymphoblastoid cell line, J-Jhan, confirmed the presence of positive and negative regulatory elements responsive to HHV-6A infection in LMP-1 regulatory sequence (LRS). The majority of LRS constructs were under the influence of dominant negative factors. HHV-6A was able to override the effect of such factors acting on reporter plasmids containing the -634/-54, -324/-54, -214/-54, and -106/-54 parts of LRS. The plasmid that carried only the -54/+40 LRS region was constitutively active in both Akata and J-Jhan cells; in Akata, its activity was influenced by HHV-6A. The finding that HHV-6A infection may activate LMP-1 and EBNA-2 expression, which is essential for the immortalization of B-lymphocytes by EBV, shows a novel aspect of the interaction between these two herpesviruses. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/9624610/Upregulation_of_Epstein_Barr_virus_encoded_latent_membrane_protein_by_human_herpesvirus_6_superinfection_of_EBV_carrying_Burkitt_lymphoma_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=1998&volume=55&issue=3&spage=219 DB - PRIME DP - Unbound Medicine ER -