Tags

Type your tag names separated by a space and hit enter

Enhancement of antioxidative ability of rat plasma by oral administration of (-)-epicatechin.
Free Radic Biol Med. 1998 May; 24(7-8):1209-16.FR

Abstract

To check whether ingestion of (-)-epicatechin (EC) affects the antioxidative defense in blood plasma, we studied the oxidizability of plasma from Wistar rats after intragastrical EC administration at 10 or 50 mg/rat. The plasma pool obtained from control or EC-administered rats was oxidized with copper sulfate or 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH). EC metabolites in plasma 1 h after EC administration contained glucuronide and glucuronide-sulfate conjugates in both the free and O-methylated form. After 6 h, the plasma concentration of total EC metabolites decreased and the remaining conjugates were mostly present as the O-methylated form. Compared to the control group, the plasma obtained from rats 1 and 6 h after EC administration was more resistant against copper sulfate-induced oxidation on the basis of cholesteryl ester hydroperoxide (CE-OOH) accumulation. Also, the consumption of alpha-tocopherol during oxidation was suppressed in the plasma obtained from EC-treated rats. The content of CE-OOH and consumption of alpha-tocopherol in the plasma from EC-administered animals was much lower than those expected from the amount of nonmetabolized EC present in the plasma. Similar results were obtained from AAPH-induced oxidation of rat plasma after EC administration, except for the fact that CE-OOH accumulation was less suppressed in the plasma 6 h following administration. The O-methylated form was found to be more stable than the free form when EC-administered rat plasma was auto-oxidized at 37 degrees. These results suggest that EC metabolites, particularly conjugates in the free form, possess an effective antioxidative activity in blood plasma.

Authors+Show Affiliations

National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9626576

Citation

Da Silva, E L., et al. "Enhancement of Antioxidative Ability of Rat Plasma By Oral Administration of (-)-epicatechin." Free Radical Biology & Medicine, vol. 24, no. 7-8, 1998, pp. 1209-16.
Da Silva EL, Piskula M, Terao J. Enhancement of antioxidative ability of rat plasma by oral administration of (-)-epicatechin. Free Radic Biol Med. 1998;24(7-8):1209-16.
Da Silva, E. L., Piskula, M., & Terao, J. (1998). Enhancement of antioxidative ability of rat plasma by oral administration of (-)-epicatechin. Free Radical Biology & Medicine, 24(7-8), 1209-16.
Da Silva EL, Piskula M, Terao J. Enhancement of Antioxidative Ability of Rat Plasma By Oral Administration of (-)-epicatechin. Free Radic Biol Med. 1998;24(7-8):1209-16. PubMed PMID: 9626576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement of antioxidative ability of rat plasma by oral administration of (-)-epicatechin. AU - Da Silva,E L, AU - Piskula,M, AU - Terao,J, PY - 1998/6/17/pubmed PY - 1998/6/17/medline PY - 1998/6/17/entrez SP - 1209 EP - 16 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 24 IS - 7-8 N2 - To check whether ingestion of (-)-epicatechin (EC) affects the antioxidative defense in blood plasma, we studied the oxidizability of plasma from Wistar rats after intragastrical EC administration at 10 or 50 mg/rat. The plasma pool obtained from control or EC-administered rats was oxidized with copper sulfate or 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH). EC metabolites in plasma 1 h after EC administration contained glucuronide and glucuronide-sulfate conjugates in both the free and O-methylated form. After 6 h, the plasma concentration of total EC metabolites decreased and the remaining conjugates were mostly present as the O-methylated form. Compared to the control group, the plasma obtained from rats 1 and 6 h after EC administration was more resistant against copper sulfate-induced oxidation on the basis of cholesteryl ester hydroperoxide (CE-OOH) accumulation. Also, the consumption of alpha-tocopherol during oxidation was suppressed in the plasma obtained from EC-treated rats. The content of CE-OOH and consumption of alpha-tocopherol in the plasma from EC-administered animals was much lower than those expected from the amount of nonmetabolized EC present in the plasma. Similar results were obtained from AAPH-induced oxidation of rat plasma after EC administration, except for the fact that CE-OOH accumulation was less suppressed in the plasma 6 h following administration. The O-methylated form was found to be more stable than the free form when EC-administered rat plasma was auto-oxidized at 37 degrees. These results suggest that EC metabolites, particularly conjugates in the free form, possess an effective antioxidative activity in blood plasma. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/9626576/Enhancement_of_antioxidative_ability_of_rat_plasma_by_oral_administration_of_____epicatechin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(97)00438-3 DB - PRIME DP - Unbound Medicine ER -