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Microglia-specific localisation of a novel calcium binding protein, Iba1.

Abstract

Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells [Imai et al. Biophys. Biochem. Res. Commun., 224 (1996) 855-862]. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain, respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo, and that Iba1 protein plays a role in regulating the function of microglia, especially in the activated microglia.

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  • Authors+Show Affiliations

    ,

    Department of Neurochemistry, National Institute of Neuroscience, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan.

    , , , ,

    Source

    MeSH

    Animals
    Axotomy
    Brain
    Calcium-Binding Proteins
    Cells, Cultured
    Facial Nerve
    Fluorescent Antibody Technique
    Microfilament Proteins
    Microglia
    Motor Neurons
    Rats
    Rats, Wistar

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    9630473

    Citation

    Ito, D, et al. "Microglia-specific Localisation of a Novel Calcium Binding Protein, Iba1." Brain Research. Molecular Brain Research, vol. 57, no. 1, 1998, pp. 1-9.
    Ito D, Imai Y, Ohsawa K, et al. Microglia-specific localisation of a novel calcium binding protein, Iba1. Brain Res Mol Brain Res. 1998;57(1):1-9.
    Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y., & Kohsaka, S. (1998). Microglia-specific localisation of a novel calcium binding protein, Iba1. Brain Research. Molecular Brain Research, 57(1), pp. 1-9.
    Ito D, et al. Microglia-specific Localisation of a Novel Calcium Binding Protein, Iba1. Brain Res Mol Brain Res. 1998 Jun 1;57(1):1-9. PubMed PMID: 9630473.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Microglia-specific localisation of a novel calcium binding protein, Iba1. AU - Ito,D, AU - Imai,Y, AU - Ohsawa,K, AU - Nakajima,K, AU - Fukuuchi,Y, AU - Kohsaka,S, PY - 1998/6/19/pubmed PY - 1998/6/19/medline PY - 1998/6/19/entrez SP - 1 EP - 9 JF - Brain research. Molecular brain research JO - Brain Res. Mol. Brain Res. VL - 57 IS - 1 N2 - Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells [Imai et al. Biophys. Biochem. Res. Commun., 224 (1996) 855-862]. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain, respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo, and that Iba1 protein plays a role in regulating the function of microglia, especially in the activated microglia. SN - 0169-328X UR - https://www.unboundmedicine.com/medline/citation/9630473/Microglia_specific_localisation_of_a_novel_calcium_binding_protein_Iba1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0169328X98000400 DB - PRIME DP - Unbound Medicine ER -