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Effect on product formation in recombinant Saccharomyces cerevisiae strains expressing different levels of xylose metabolic genes.
Chin J Biotechnol. 1997; 13(4):225-31.CJ

Abstract

The XYL1 and XYL2 genes from Pichia stipitis encoding xylose reductase (XR) and xylilitol dehydrogenase (XDH), respectively, were transformed into Saccharomyces cerevisiae. These two genes were placed in different directions under the control of the alcohol dehydrogenase I (ADHI) and phosphoglycerate kinase (PGK) promoters and inserted into the E. coli-yeast shuttle plasmid YEp24. Different recombinant S. cerevisiae strains were constructed with different specific activities of XR and XDH. The highest XR or XDH activities were obtained when the expressed gene was controlled by the PGK promoter and located downstream after the ADHI promoter-gene-terminator sequence. The XR/XDH ratio (ratio of specific enzyme activities of XR and XDH) in these recombinant S. cerevisiae strains varied from 17.5 to 0.06. In order to enhance xylose utilization, in the XYL1, XYL2 containing S. cerevisiae strains, the native TKL1 gene encoding transketolase and the TALI gene encoding transaldolase were also overexpressed, which showed considerably good growth on the xylose plate. Fermentation of the recombinant S. cerevisiae strains containing XYL1, XYL2, TKL1, and TAL1 were studied with mixtures of glucose and xylose. The strain with XR/XDH ratio of 0.06 consumed 3.25 g/L xylose and formed no xylitol and less glycerol and acetic acid, but more ethanol compared with the strains with a higher XR/XDH ratio.

Authors+Show Affiliations

Department of Microbiology, State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9631257

Citation

Bao, X, et al. "Effect On Product Formation in Recombinant Saccharomyces Cerevisiae Strains Expressing Different Levels of Xylose Metabolic Genes." Chinese Journal of Biotechnology, vol. 13, no. 4, 1997, pp. 225-31.
Bao X, Gao D, Qu Y, et al. Effect on product formation in recombinant Saccharomyces cerevisiae strains expressing different levels of xylose metabolic genes. Chin J Biotechnol. 1997;13(4):225-31.
Bao, X., Gao, D., Qu, Y., Wang, Z., Walfridssion, M., & Hahn-Hagerbal, B. (1997). Effect on product formation in recombinant Saccharomyces cerevisiae strains expressing different levels of xylose metabolic genes. Chinese Journal of Biotechnology, 13(4), 225-31.
Bao X, et al. Effect On Product Formation in Recombinant Saccharomyces Cerevisiae Strains Expressing Different Levels of Xylose Metabolic Genes. Chin J Biotechnol. 1997;13(4):225-31. PubMed PMID: 9631257.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect on product formation in recombinant Saccharomyces cerevisiae strains expressing different levels of xylose metabolic genes. AU - Bao,X, AU - Gao,D, AU - Qu,Y, AU - Wang,Z, AU - Walfridssion,M, AU - Hahn-Hagerbal,B, PY - 1997/1/1/pubmed PY - 1998/6/19/medline PY - 1997/1/1/entrez SP - 225 EP - 31 JF - Chinese journal of biotechnology JO - Chin J Biotechnol VL - 13 IS - 4 N2 - The XYL1 and XYL2 genes from Pichia stipitis encoding xylose reductase (XR) and xylilitol dehydrogenase (XDH), respectively, were transformed into Saccharomyces cerevisiae. These two genes were placed in different directions under the control of the alcohol dehydrogenase I (ADHI) and phosphoglycerate kinase (PGK) promoters and inserted into the E. coli-yeast shuttle plasmid YEp24. Different recombinant S. cerevisiae strains were constructed with different specific activities of XR and XDH. The highest XR or XDH activities were obtained when the expressed gene was controlled by the PGK promoter and located downstream after the ADHI promoter-gene-terminator sequence. The XR/XDH ratio (ratio of specific enzyme activities of XR and XDH) in these recombinant S. cerevisiae strains varied from 17.5 to 0.06. In order to enhance xylose utilization, in the XYL1, XYL2 containing S. cerevisiae strains, the native TKL1 gene encoding transketolase and the TALI gene encoding transaldolase were also overexpressed, which showed considerably good growth on the xylose plate. Fermentation of the recombinant S. cerevisiae strains containing XYL1, XYL2, TKL1, and TAL1 were studied with mixtures of glucose and xylose. The strain with XR/XDH ratio of 0.06 consumed 3.25 g/L xylose and formed no xylitol and less glycerol and acetic acid, but more ethanol compared with the strains with a higher XR/XDH ratio. SN - 1042-749X UR - https://www.unboundmedicine.com/medline/citation/9631257/Effect_on_product_formation_in_recombinant_Saccharomyces_cerevisiae_strains_expressing_different_levels_of_xylose_metabolic_genes_ L2 - https://www.yeastgenome.org/reference/9631257 DB - PRIME DP - Unbound Medicine ER -