Tags

Type your tag names separated by a space and hit enter

Gas chromatography-electron ionization and chemical ionization mass spectrometric analysis of serum mexiletine concentration after derivatization with 2,2,2-trichloroethyl chloroformate: a novel derivative.
Ther Drug Monit. 1998 Jun; 20(3):313-8.TD

Abstract

Mexiletine (Mexitil) is an antiarrhythmic agent used in the treatment of ventricular arrhythmia. The drug has a narrow therapeutic window, and monitoring its serum concentration is recommended. The authors describe a gas chromatography-mass spectrometric (GC/MS) assay of mexiletine using selected ion monitoring. Mexiletine was extracted from alkaline serum with dichloromethane followed by derivatization with 2,2,2-trichloroethyl chloroformate. The reaction was completed in 30 minutes at 70 degrees C. N-propylamphetamine was used as the internal standard. The ions monitored were m/z 58, 102, 122, 232, and 234 for derivatized mexiletine and m/z 56, 91, 131, 260, and 262 for the derivatized internal standard. The within-run precision at a serum mexiletine concentration of 1 mg/l was 1.7% (mean = 0.981, SD = 0.017 mg/l, n = 8) and the between-run precision was 3.3% (mean = 0.983, SD = 0.033 mg/l, n = 6). The assay was linear for serum mexiletine concentrations of 0.2 to 2.5 mg/l. The detection limit was 0.1 mg/l. The authors observed no carry-over problem in their assay. They observed a good correlation between mexiletine concentrations measured by a reference laboratory (Associated Regional University Pathologists, Salt Lake City, UT, U.S.A.) and by the new GC/MS assay.

Authors+Show Affiliations

Department of Pathology and Laboratory Medicine, University of Texas-Houston Health Science Center, 77030, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9631929

Citation

Dasgupta, A, et al. "Gas Chromatography-electron Ionization and Chemical Ionization Mass Spectrometric Analysis of Serum Mexiletine Concentration After Derivatization With 2,2,2-trichloroethyl Chloroformate: a Novel Derivative." Therapeutic Drug Monitoring, vol. 20, no. 3, 1998, pp. 313-8.
Dasgupta A, Appenzeller P, Moore J. Gas chromatography-electron ionization and chemical ionization mass spectrometric analysis of serum mexiletine concentration after derivatization with 2,2,2-trichloroethyl chloroformate: a novel derivative. Ther Drug Monit. 1998;20(3):313-8.
Dasgupta, A., Appenzeller, P., & Moore, J. (1998). Gas chromatography-electron ionization and chemical ionization mass spectrometric analysis of serum mexiletine concentration after derivatization with 2,2,2-trichloroethyl chloroformate: a novel derivative. Therapeutic Drug Monitoring, 20(3), 313-8.
Dasgupta A, Appenzeller P, Moore J. Gas Chromatography-electron Ionization and Chemical Ionization Mass Spectrometric Analysis of Serum Mexiletine Concentration After Derivatization With 2,2,2-trichloroethyl Chloroformate: a Novel Derivative. Ther Drug Monit. 1998;20(3):313-8. PubMed PMID: 9631929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gas chromatography-electron ionization and chemical ionization mass spectrometric analysis of serum mexiletine concentration after derivatization with 2,2,2-trichloroethyl chloroformate: a novel derivative. AU - Dasgupta,A, AU - Appenzeller,P, AU - Moore,J, PY - 1998/6/19/pubmed PY - 1998/6/19/medline PY - 1998/6/19/entrez SP - 313 EP - 8 JF - Therapeutic drug monitoring JO - Ther Drug Monit VL - 20 IS - 3 N2 - Mexiletine (Mexitil) is an antiarrhythmic agent used in the treatment of ventricular arrhythmia. The drug has a narrow therapeutic window, and monitoring its serum concentration is recommended. The authors describe a gas chromatography-mass spectrometric (GC/MS) assay of mexiletine using selected ion monitoring. Mexiletine was extracted from alkaline serum with dichloromethane followed by derivatization with 2,2,2-trichloroethyl chloroformate. The reaction was completed in 30 minutes at 70 degrees C. N-propylamphetamine was used as the internal standard. The ions monitored were m/z 58, 102, 122, 232, and 234 for derivatized mexiletine and m/z 56, 91, 131, 260, and 262 for the derivatized internal standard. The within-run precision at a serum mexiletine concentration of 1 mg/l was 1.7% (mean = 0.981, SD = 0.017 mg/l, n = 8) and the between-run precision was 3.3% (mean = 0.983, SD = 0.033 mg/l, n = 6). The assay was linear for serum mexiletine concentrations of 0.2 to 2.5 mg/l. The detection limit was 0.1 mg/l. The authors observed no carry-over problem in their assay. They observed a good correlation between mexiletine concentrations measured by a reference laboratory (Associated Regional University Pathologists, Salt Lake City, UT, U.S.A.) and by the new GC/MS assay. SN - 0163-4356 UR - https://www.unboundmedicine.com/medline/citation/9631929/Gas_chromatography_electron_ionization_and_chemical_ionization_mass_spectrometric_analysis_of_serum_mexiletine_concentration_after_derivatization_with_222_trichloroethyl_chloroformate:_a_novel_derivative_ L2 - http://dx.doi.org/10.1097/00007691-199806000-00012 DB - PRIME DP - Unbound Medicine ER -