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A hybrid baculovirus-bacteriophage T7 transient expression system.
Biotechnology (N Y). 1995 Mar; 13(3):261-4.B

Abstract

A hybrid recombinant baculovirus-bacteriophage T7 expression system was developed for transient expression in insect cells of plasmids with foreign genes provided with a T7 promoter. The coding sequence for T7 RNA polymerase, with or without a nuclear localization signal, was inserted into the genome of Autographa californica nuclear polyhedrosis virus. Recombinant viruses stably expressed T7 RNA polymerase in insect cells. Upon transfection of infected insect cells with plasmids containing the genes for chloramphenicol acetyltransferase (CAT), the hepatitis B virus precore-, core- or e- antigens under control of the T7 promoter, transient expression of these genes was detected by ELISA. The results obtained indicate that this baculovirus/T7 system provides a simple and widely applicable tool for transient gene expression studies.

Authors+Show Affiliations

Department of Virology, Binnenhaven, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9634768

Citation

van Poelwijk, F, et al. "A Hybrid Baculovirus-bacteriophage T7 Transient Expression System." Bio/technology (Nature Publishing Company), vol. 13, no. 3, 1995, pp. 261-4.
van Poelwijk F, Broer R, Belsham GJ, et al. A hybrid baculovirus-bacteriophage T7 transient expression system. Biotechnology (NY). 1995;13(3):261-4.
van Poelwijk, F., Broer, R., Belsham, G. J., Oudshoorn, P., Vlak, J. M., & Goldbach, R. W. (1995). A hybrid baculovirus-bacteriophage T7 transient expression system. Bio/technology (Nature Publishing Company), 13(3), 261-4.
van Poelwijk F, et al. A Hybrid Baculovirus-bacteriophage T7 Transient Expression System. Biotechnology (NY). 1995;13(3):261-4. PubMed PMID: 9634768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A hybrid baculovirus-bacteriophage T7 transient expression system. AU - van Poelwijk,F, AU - Broer,R, AU - Belsham,G J, AU - Oudshoorn,P, AU - Vlak,J M, AU - Goldbach,R W, PY - 1995/3/1/pubmed PY - 1998/6/23/medline PY - 1995/3/1/entrez SP - 261 EP - 4 JF - Bio/technology (Nature Publishing Company) JO - Biotechnology (N.Y.) VL - 13 IS - 3 N2 - A hybrid recombinant baculovirus-bacteriophage T7 expression system was developed for transient expression in insect cells of plasmids with foreign genes provided with a T7 promoter. The coding sequence for T7 RNA polymerase, with or without a nuclear localization signal, was inserted into the genome of Autographa californica nuclear polyhedrosis virus. Recombinant viruses stably expressed T7 RNA polymerase in insect cells. Upon transfection of infected insect cells with plasmids containing the genes for chloramphenicol acetyltransferase (CAT), the hepatitis B virus precore-, core- or e- antigens under control of the T7 promoter, transient expression of these genes was detected by ELISA. The results obtained indicate that this baculovirus/T7 system provides a simple and widely applicable tool for transient gene expression studies. SN - 0733-222X UR - https://www.unboundmedicine.com/medline/citation/9634768/A_hybrid_baculovirus_bacteriophage_T7_transient_expression_system_ DB - PRIME DP - Unbound Medicine ER -