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The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways.
J Immunol 1998; 160(12):5735-41JI

Abstract

Signals from the IL-7R are essential for normal thymocyte development. We isolated thymocytes from early developmental stages and observed that suspensions of pro-T1, -T2, and -T3 cells rapidly died in culture. Addition of IL-7 promoted their survival, but did not induce cell division. Pro-T4 cells did not undergo rapid cell death, and their survival was therefore independent of IL-7. Death in the absence of IL-7 showed the hallmarks of apoptosis, including DNA fragmentation and annexin V binding; however, caspase inhibitors blocked DNA fragmentation, but did not block cell death. The trophic effect of IL-7 was partially inhibited by blocking protein synthesis. The p53 pathway was not involved in this death pathway, since pro-T cells from p53-/- mice also underwent cell death in the absence of IL-7. The Fas/Fas ligand pathway was not involved in cell death, since Fas-deficient pro-T cells died normally in the absence of IL-7, anti-Fas Abs did not protect cells from death in the absence of IL-7, and Fas expression was undetectable on cells at these stages. The IL-7 trophic affect correlated with increased intracellular levels of Bcl-2 and decreased levels of Bax, whereas no Bcl-X(L), Bcl-w, or Bad was detectable. Thus, maintaining a favorable Bcl-2/Bax ratio may account for the trophic action of IL-7.

Authors+Show Affiliations

Laboratory of Molecular Immunoregulation, Division of Basic Sciences, National Cancer Institute, Frederick, MD 21702, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9637482

Citation

Kim, K, et al. "The Trophic Action of IL-7 On pro-T Cells: Inhibition of Apoptosis of pro-T1, -T2, and -T3 Cells Correlates With Bcl-2 and Bax Levels and Is Independent of Fas and P53 Pathways." Journal of Immunology (Baltimore, Md. : 1950), vol. 160, no. 12, 1998, pp. 5735-41.
Kim K, Lee CK, Sayers TJ, et al. The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways. J Immunol. 1998;160(12):5735-41.
Kim, K., Lee, C. K., Sayers, T. J., Muegge, K., & Durum, S. K. (1998). The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways. Journal of Immunology (Baltimore, Md. : 1950), 160(12), pp. 5735-41.
Kim K, et al. The Trophic Action of IL-7 On pro-T Cells: Inhibition of Apoptosis of pro-T1, -T2, and -T3 Cells Correlates With Bcl-2 and Bax Levels and Is Independent of Fas and P53 Pathways. J Immunol. 1998 Jun 15;160(12):5735-41. PubMed PMID: 9637482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways. AU - Kim,K, AU - Lee,C K, AU - Sayers,T J, AU - Muegge,K, AU - Durum,S K, PY - 1998/6/24/pubmed PY - 1998/6/24/medline PY - 1998/6/24/entrez SP - 5735 EP - 41 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 160 IS - 12 N2 - Signals from the IL-7R are essential for normal thymocyte development. We isolated thymocytes from early developmental stages and observed that suspensions of pro-T1, -T2, and -T3 cells rapidly died in culture. Addition of IL-7 promoted their survival, but did not induce cell division. Pro-T4 cells did not undergo rapid cell death, and their survival was therefore independent of IL-7. Death in the absence of IL-7 showed the hallmarks of apoptosis, including DNA fragmentation and annexin V binding; however, caspase inhibitors blocked DNA fragmentation, but did not block cell death. The trophic effect of IL-7 was partially inhibited by blocking protein synthesis. The p53 pathway was not involved in this death pathway, since pro-T cells from p53-/- mice also underwent cell death in the absence of IL-7. The Fas/Fas ligand pathway was not involved in cell death, since Fas-deficient pro-T cells died normally in the absence of IL-7, anti-Fas Abs did not protect cells from death in the absence of IL-7, and Fas expression was undetectable on cells at these stages. The IL-7 trophic affect correlated with increased intracellular levels of Bcl-2 and decreased levels of Bax, whereas no Bcl-X(L), Bcl-w, or Bad was detectable. Thus, maintaining a favorable Bcl-2/Bax ratio may account for the trophic action of IL-7. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9637482/The_trophic_action_of_IL_7_on_pro_T_cells:_inhibition_of_apoptosis_of_pro_T1__T2_and__T3_cells_correlates_with_Bcl_2_and_Bax_levels_and_is_independent_of_Fas_and_p53_pathways_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9637482 DB - PRIME DP - Unbound Medicine ER -