Tags

Type your tag names separated by a space and hit enter

The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways.

Abstract

Signals from the IL-7R are essential for normal thymocyte development. We isolated thymocytes from early developmental stages and observed that suspensions of pro-T1, -T2, and -T3 cells rapidly died in culture. Addition of IL-7 promoted their survival, but did not induce cell division. Pro-T4 cells did not undergo rapid cell death, and their survival was therefore independent of IL-7. Death in the absence of IL-7 showed the hallmarks of apoptosis, including DNA fragmentation and annexin V binding; however, caspase inhibitors blocked DNA fragmentation, but did not block cell death. The trophic effect of IL-7 was partially inhibited by blocking protein synthesis. The p53 pathway was not involved in this death pathway, since pro-T cells from p53-/- mice also underwent cell death in the absence of IL-7. The Fas/Fas ligand pathway was not involved in cell death, since Fas-deficient pro-T cells died normally in the absence of IL-7, anti-Fas Abs did not protect cells from death in the absence of IL-7, and Fas expression was undetectable on cells at these stages. The IL-7 trophic affect correlated with increased intracellular levels of Bcl-2 and decreased levels of Bax, whereas no Bcl-X(L), Bcl-w, or Bad was detectable. Thus, maintaining a favorable Bcl-2/Bax ratio may account for the trophic action of IL-7.

Links

  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Laboratory of Molecular Immunoregulation, Division of Basic Sciences, National Cancer Institute, Frederick, MD 21702, USA.

    , , ,

    Source

    Journal of immunology (Baltimore, Md. : 1950) 160:12 1998 Jun 15 pg 5735-41

    MeSH

    Animals
    Antigens, CD
    Apoptosis
    Cell Cycle
    Cell Death
    Cell Survival
    Cells, Cultured
    Hematopoietic Stem Cells
    Interleukin-7
    Mice
    Mice, Inbred C57BL
    Mice, Inbred MRL lpr
    Proto-Oncogene Proteins
    Proto-Oncogene Proteins c-bcl-2
    Receptors, Interleukin
    Receptors, Interleukin-7
    Signal Transduction
    T-Lymphocytes
    Thymus Gland
    Tumor Suppressor Protein p53
    bcl-2-Associated X Protein
    fas Receptor

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    9637482

    Citation

    Kim, K, et al. "The Trophic Action of IL-7 On pro-T Cells: Inhibition of Apoptosis of pro-T1, -T2, and -T3 Cells Correlates With Bcl-2 and Bax Levels and Is Independent of Fas and P53 Pathways." Journal of Immunology (Baltimore, Md. : 1950), vol. 160, no. 12, 1998, pp. 5735-41.
    Kim K, Lee CK, Sayers TJ, et al. The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways. J Immunol. 1998;160(12):5735-41.
    Kim, K., Lee, C. K., Sayers, T. J., Muegge, K., & Durum, S. K. (1998). The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways. Journal of Immunology (Baltimore, Md. : 1950), 160(12), pp. 5735-41.
    Kim K, et al. The Trophic Action of IL-7 On pro-T Cells: Inhibition of Apoptosis of pro-T1, -T2, and -T3 Cells Correlates With Bcl-2 and Bax Levels and Is Independent of Fas and P53 Pathways. J Immunol. 1998 Jun 15;160(12):5735-41. PubMed PMID: 9637482.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The trophic action of IL-7 on pro-T cells: inhibition of apoptosis of pro-T1, -T2, and -T3 cells correlates with Bcl-2 and Bax levels and is independent of Fas and p53 pathways. AU - Kim,K, AU - Lee,C K, AU - Sayers,T J, AU - Muegge,K, AU - Durum,S K, PY - 1998/6/24/pubmed PY - 1998/6/24/medline PY - 1998/6/24/entrez SP - 5735 EP - 41 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 160 IS - 12 N2 - Signals from the IL-7R are essential for normal thymocyte development. We isolated thymocytes from early developmental stages and observed that suspensions of pro-T1, -T2, and -T3 cells rapidly died in culture. Addition of IL-7 promoted their survival, but did not induce cell division. Pro-T4 cells did not undergo rapid cell death, and their survival was therefore independent of IL-7. Death in the absence of IL-7 showed the hallmarks of apoptosis, including DNA fragmentation and annexin V binding; however, caspase inhibitors blocked DNA fragmentation, but did not block cell death. The trophic effect of IL-7 was partially inhibited by blocking protein synthesis. The p53 pathway was not involved in this death pathway, since pro-T cells from p53-/- mice also underwent cell death in the absence of IL-7. The Fas/Fas ligand pathway was not involved in cell death, since Fas-deficient pro-T cells died normally in the absence of IL-7, anti-Fas Abs did not protect cells from death in the absence of IL-7, and Fas expression was undetectable on cells at these stages. The IL-7 trophic affect correlated with increased intracellular levels of Bcl-2 and decreased levels of Bax, whereas no Bcl-X(L), Bcl-w, or Bad was detectable. Thus, maintaining a favorable Bcl-2/Bax ratio may account for the trophic action of IL-7. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9637482/The_trophic_action_of_IL_7_on_pro_T_cells:_inhibition_of_apoptosis_of_pro_T1__T2_and__T3_cells_correlates_with_Bcl_2_and_Bax_levels_and_is_independent_of_Fas_and_p53_pathways_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9637482 DB - PRIME DP - Unbound Medicine ER -