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Effects of dietary fish oil on serum lipids and VLDL kinetics in hyperlipidemic apolipoprotein E*3-Leiden transgenic mice.
J Lipid Res 1998; 39(6):1181-8JL

Abstract

Studying the effects of dietary fish oil on VLDL metabolism in humans is subject to both large intra- and interindividual variability. In the present study we therefore used hyperlipidemic apolipoprotein (APO) E*3-Leiden mice, which have impaired chylomicron and very low density lipoprotein (VLDL) remnant metabolism, to study the effects of dietary fish oil on serum lipids and VLDL kinetics under highly standardized conditions. For this, female APOE*3-Leiden mice were fed a fat- and cholesterol-containing diet supplemented with either 0, 3 or 6% w/w (i.e. 0, 6, or 12% of total energy) of fish oil. Fish oil-fed mice showed a significant dose-dependent decrease in serum cholesterol (up to -43%) and triglyceride levels (up to -60%), mainly due to a reduction of VLDL (-80%). LDL and HDL cholesterol levels were not affected by fish oil feeding. VLDL-apoB kinetic studies showed that fish oil feeding resulted in a significant 2-fold increase in VLDL-apoB fractional catabolic rate (FCR). Hepatic VLDL-apoB production was, however, not affected by fish oil feeding. VLDL-triglyceride turnover studies revealed that fish oil significantly decreased hepatic VLDL-triglyceride production rate (-60%). A significant increase in VLDL-triglyceride FCR was observed (+70%), which was not related to increased lipolytic activity. We conclude that APOE*3-Leiden mice are highly responsive to dietary fish oil. The observed strong reduction in serum very low density lipoprotein (VLDL) is primarily due to an effect of fish oil to decrease hepatic VLDL triglyceride production rate and to increase VLDL-apoB fractional catabolic rate.

Authors+Show Affiliations

TNO Prevention and Health, Gaubius Laboratory, Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9643349

Citation

van Vlijmen, B J., et al. "Effects of Dietary Fish Oil On Serum Lipids and VLDL Kinetics in Hyperlipidemic Apolipoprotein E*3-Leiden Transgenic Mice." Journal of Lipid Research, vol. 39, no. 6, 1998, pp. 1181-8.
van Vlijmen BJ, Mensink RP, van 't Hof HB, et al. Effects of dietary fish oil on serum lipids and VLDL kinetics in hyperlipidemic apolipoprotein E*3-Leiden transgenic mice. J Lipid Res. 1998;39(6):1181-8.
van Vlijmen, B. J., Mensink, R. P., van 't Hof, H. B., Offermans, R. F., Hofker, M. H., & Havekes, L. M. (1998). Effects of dietary fish oil on serum lipids and VLDL kinetics in hyperlipidemic apolipoprotein E*3-Leiden transgenic mice. Journal of Lipid Research, 39(6), pp. 1181-8.
van Vlijmen BJ, et al. Effects of Dietary Fish Oil On Serum Lipids and VLDL Kinetics in Hyperlipidemic Apolipoprotein E*3-Leiden Transgenic Mice. J Lipid Res. 1998;39(6):1181-8. PubMed PMID: 9643349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary fish oil on serum lipids and VLDL kinetics in hyperlipidemic apolipoprotein E*3-Leiden transgenic mice. AU - van Vlijmen,B J, AU - Mensink,R P, AU - van 't Hof,H B, AU - Offermans,R F, AU - Hofker,M H, AU - Havekes,L M, PY - 1998/6/27/pubmed PY - 1998/6/27/medline PY - 1998/6/27/entrez SP - 1181 EP - 8 JF - Journal of lipid research JO - J. Lipid Res. VL - 39 IS - 6 N2 - Studying the effects of dietary fish oil on VLDL metabolism in humans is subject to both large intra- and interindividual variability. In the present study we therefore used hyperlipidemic apolipoprotein (APO) E*3-Leiden mice, which have impaired chylomicron and very low density lipoprotein (VLDL) remnant metabolism, to study the effects of dietary fish oil on serum lipids and VLDL kinetics under highly standardized conditions. For this, female APOE*3-Leiden mice were fed a fat- and cholesterol-containing diet supplemented with either 0, 3 or 6% w/w (i.e. 0, 6, or 12% of total energy) of fish oil. Fish oil-fed mice showed a significant dose-dependent decrease in serum cholesterol (up to -43%) and triglyceride levels (up to -60%), mainly due to a reduction of VLDL (-80%). LDL and HDL cholesterol levels were not affected by fish oil feeding. VLDL-apoB kinetic studies showed that fish oil feeding resulted in a significant 2-fold increase in VLDL-apoB fractional catabolic rate (FCR). Hepatic VLDL-apoB production was, however, not affected by fish oil feeding. VLDL-triglyceride turnover studies revealed that fish oil significantly decreased hepatic VLDL-triglyceride production rate (-60%). A significant increase in VLDL-triglyceride FCR was observed (+70%), which was not related to increased lipolytic activity. We conclude that APOE*3-Leiden mice are highly responsive to dietary fish oil. The observed strong reduction in serum very low density lipoprotein (VLDL) is primarily due to an effect of fish oil to decrease hepatic VLDL triglyceride production rate and to increase VLDL-apoB fractional catabolic rate. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/9643349/Effects_of_dietary_fish_oil_on_serum_lipids_and_VLDL_kinetics_in_hyperlipidemic_apolipoprotein_E_3_Leiden_transgenic_mice_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&pmid=9643349 DB - PRIME DP - Unbound Medicine ER -