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The membrane-type-matrix metalloproteinase/matrix metalloproteinase-2/tissue inhibitor of metalloproteinase-2 system in periprosthetic connective-tissue remodeling in loose total-hip prostheses.
Lab Invest. 1998 Jun; 78(6):735-42.LI

Abstract

The aim of the present study was to investigate the proteolytic potential and localization of matrix metalloproteinase (MMP)-2 in relation to its regulatory protein, membrane-type-MMP (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as to clarify an important step in the cascade of periprosthetic connective-tissue remodeling in loose total-hip prostheses. Immunohistochemical analysis revealed increased expression of MT1-MMP, MMP-2, and TIMP-2 in fibroblasts, synovial lining-like cells, and endothelial cells, as well as, to some extent, in monocyte/macrophage-like cells in both tissues from the bone-implant interface and reactive cellular tissues from regenerating capsules in loose hip joints, when compared with control fibrous tissues between bone and implants retrieved from unloosened hip joints. In loose hip joints, reverse transcription-PCR analysis showed the presence of MT1-MMP, MMP-2, and TIMP-2 mRNA in both the bone-implant interface and regenerating capsular tissues. Increased protein levels of MMP-2 and TIMP-2 were also demonstrated by an ELISA, and those of MT1-MMP were shown by immunoblot analysis. Gelatin-zymographic analysis confirmed the presence of both pro- and active forms of MMP-2, which suggested the in situ activation of MMP-2 by MT1-MMP in the loose hip joints. Collectively, these data suggest that the MT1-MMP/MMP-2/TIMP-2 system participates in the extracellular matrix degradation and periprosthetic connective-tissue remodeling in loose hip joints, and may thus contribute to the periprosthetic weakening, loosening, and osteolysis that can occur around implants.

Authors+Show Affiliations

Department of Orthopedic Surgery, Yamagata University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9645764

Citation

Takagi, M, et al. "The Membrane-type-matrix Metalloproteinase/matrix Metalloproteinase-2/tissue Inhibitor of Metalloproteinase-2 System in Periprosthetic Connective-tissue Remodeling in Loose Total-hip Prostheses." Laboratory Investigation; a Journal of Technical Methods and Pathology, vol. 78, no. 6, 1998, pp. 735-42.
Takagi M, Santavirta S, Ida H, et al. The membrane-type-matrix metalloproteinase/matrix metalloproteinase-2/tissue inhibitor of metalloproteinase-2 system in periprosthetic connective-tissue remodeling in loose total-hip prostheses. Lab Invest. 1998;78(6):735-42.
Takagi, M., Santavirta, S., Ida, H., Ishii, M., Akimoto, K., Saotome, K., & Konttinen, Y. T. (1998). The membrane-type-matrix metalloproteinase/matrix metalloproteinase-2/tissue inhibitor of metalloproteinase-2 system in periprosthetic connective-tissue remodeling in loose total-hip prostheses. Laboratory Investigation; a Journal of Technical Methods and Pathology, 78(6), 735-42.
Takagi M, et al. The Membrane-type-matrix Metalloproteinase/matrix Metalloproteinase-2/tissue Inhibitor of Metalloproteinase-2 System in Periprosthetic Connective-tissue Remodeling in Loose Total-hip Prostheses. Lab Invest. 1998;78(6):735-42. PubMed PMID: 9645764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The membrane-type-matrix metalloproteinase/matrix metalloproteinase-2/tissue inhibitor of metalloproteinase-2 system in periprosthetic connective-tissue remodeling in loose total-hip prostheses. AU - Takagi,M, AU - Santavirta,S, AU - Ida,H, AU - Ishii,M, AU - Akimoto,K, AU - Saotome,K, AU - Konttinen,Y T, PY - 1998/6/30/pubmed PY - 1998/6/30/medline PY - 1998/6/30/entrez SP - 735 EP - 42 JF - Laboratory investigation; a journal of technical methods and pathology JO - Lab Invest VL - 78 IS - 6 N2 - The aim of the present study was to investigate the proteolytic potential and localization of matrix metalloproteinase (MMP)-2 in relation to its regulatory protein, membrane-type-MMP (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as to clarify an important step in the cascade of periprosthetic connective-tissue remodeling in loose total-hip prostheses. Immunohistochemical analysis revealed increased expression of MT1-MMP, MMP-2, and TIMP-2 in fibroblasts, synovial lining-like cells, and endothelial cells, as well as, to some extent, in monocyte/macrophage-like cells in both tissues from the bone-implant interface and reactive cellular tissues from regenerating capsules in loose hip joints, when compared with control fibrous tissues between bone and implants retrieved from unloosened hip joints. In loose hip joints, reverse transcription-PCR analysis showed the presence of MT1-MMP, MMP-2, and TIMP-2 mRNA in both the bone-implant interface and regenerating capsular tissues. Increased protein levels of MMP-2 and TIMP-2 were also demonstrated by an ELISA, and those of MT1-MMP were shown by immunoblot analysis. Gelatin-zymographic analysis confirmed the presence of both pro- and active forms of MMP-2, which suggested the in situ activation of MMP-2 by MT1-MMP in the loose hip joints. Collectively, these data suggest that the MT1-MMP/MMP-2/TIMP-2 system participates in the extracellular matrix degradation and periprosthetic connective-tissue remodeling in loose hip joints, and may thus contribute to the periprosthetic weakening, loosening, and osteolysis that can occur around implants. SN - 0023-6837 UR - https://www.unboundmedicine.com/medline/citation/9645764/The_membrane_type_matrix_metalloproteinase/matrix_metalloproteinase_2/tissue_inhibitor_of_metalloproteinase_2_system_in_periprosthetic_connective_tissue_remodeling_in_loose_total_hip_prostheses_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=9645764.ui DB - PRIME DP - Unbound Medicine ER -