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In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines.
J Immunol. 1998 Jul 01; 161(1):97-105.JI

Abstract

Naive CD8 T cells can be polarized into effectors producing the type 1 cytokines IFN-gamma and IL-2 or the type 2 cytokines IL-4, IL-5, and IL-10, respectively. To study whether the polarized cytokine phenotype of the effectors is stable, we generated highly cytotoxic hemagglutinin (HA) peptide-specific CD8 Tc1 and Tc2 (cytotoxic CD8 T cells producing type 1 or type 2 cytokines) effectors from Clone-4 TCR-transgenic mice, which were adoptively transferred into syngeneic adult thymectomized irradiated and bone marrow-reconstituted recipients. The highly activated blast-size, CD25+ Tc1 and Tc2 effectors gave rise to homogeneous resting CD25- CD44(high) Ly6C(high) Ag-specific populations, which persisted for at least 13 wk after adoptive transfer. These memory CD8 T cells, recovered 13 wk after transfer of Tc1 or Tc2 effectors, still produced either the type 1 or type 2 cytokines, i.e., IFN-gamma, or IL-4 and IL-5, respectively, upon restimulation with APCs loaded with the HA peptide, but not in the absence of Ag. The amounts of IL-2 detected in the supernatants of Tc1 and Tc2 memory populations were comparable to that in memory CD4 cells, and both Tc1 and Tc2 memory cells became cytotoxic upon restimulation. Thus, cytokine-polarized CD8 memory T cells are a source of a variety of cytokines, which were classically considered helper cytokines, opening new perspectives on their function as regulatory cells in an immune response.

Authors+Show Affiliations

Trudeau Institute, Saranac Lake, NY 12983, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9647212

Citation

Cerwenka, A, et al. "In Vivo Persistence of CD8 Polarized T Cell Subsets Producing Type 1 or Type 2 Cytokines." Journal of Immunology (Baltimore, Md. : 1950), vol. 161, no. 1, 1998, pp. 97-105.
Cerwenka A, Carter LL, Reome JB, et al. In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines. J Immunol. 1998;161(1):97-105.
Cerwenka, A., Carter, L. L., Reome, J. B., Swain, S. L., & Dutton, R. W. (1998). In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines. Journal of Immunology (Baltimore, Md. : 1950), 161(1), 97-105.
Cerwenka A, et al. In Vivo Persistence of CD8 Polarized T Cell Subsets Producing Type 1 or Type 2 Cytokines. J Immunol. 1998 Jul 1;161(1):97-105. PubMed PMID: 9647212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines. AU - Cerwenka,A, AU - Carter,L L, AU - Reome,J B, AU - Swain,S L, AU - Dutton,R W, PY - 1998/7/1/pubmed PY - 1998/7/1/medline PY - 1998/7/1/entrez SP - 97 EP - 105 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 161 IS - 1 N2 - Naive CD8 T cells can be polarized into effectors producing the type 1 cytokines IFN-gamma and IL-2 or the type 2 cytokines IL-4, IL-5, and IL-10, respectively. To study whether the polarized cytokine phenotype of the effectors is stable, we generated highly cytotoxic hemagglutinin (HA) peptide-specific CD8 Tc1 and Tc2 (cytotoxic CD8 T cells producing type 1 or type 2 cytokines) effectors from Clone-4 TCR-transgenic mice, which were adoptively transferred into syngeneic adult thymectomized irradiated and bone marrow-reconstituted recipients. The highly activated blast-size, CD25+ Tc1 and Tc2 effectors gave rise to homogeneous resting CD25- CD44(high) Ly6C(high) Ag-specific populations, which persisted for at least 13 wk after adoptive transfer. These memory CD8 T cells, recovered 13 wk after transfer of Tc1 or Tc2 effectors, still produced either the type 1 or type 2 cytokines, i.e., IFN-gamma, or IL-4 and IL-5, respectively, upon restimulation with APCs loaded with the HA peptide, but not in the absence of Ag. The amounts of IL-2 detected in the supernatants of Tc1 and Tc2 memory populations were comparable to that in memory CD4 cells, and both Tc1 and Tc2 memory cells became cytotoxic upon restimulation. Thus, cytokine-polarized CD8 memory T cells are a source of a variety of cytokines, which were classically considered helper cytokines, opening new perspectives on their function as regulatory cells in an immune response. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9647212/In_vivo_persistence_of_CD8_polarized_T_cell_subsets_producing_type_1_or_type_2_cytokines_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9647212 DB - PRIME DP - Unbound Medicine ER -