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CD4+ T cells mature in the absence of MHC class I and class II expression in Ly-6A.2 transgenic mice.
J Immunol. 1998 Jul 01; 161(1):175-82.JI

Abstract

The TCRs expressed on T lymphocytes recognize foreign peptides bound to MHC molecules. This reactivity is the basis of specific immune response to the foreign Ag. How such specificities are generated in the thymus is still being debated. Signals generated through TCR upon interaction with self MHC-peptide complexes are critical for maturation of the CD4+ helper and CD8+ cytotoxic subsets. We have observed maturation of CD4+ but not CD8+ T cells in Ly-6A.2 transgenic MHC null mice. Since there can be no interactions with MHC molecules in these mice, these CD4+ cells must express the T cell repertoire that exists before positive and negative selection. Interestingly, despite an absence of selection by MHC molecules, the CD4+ cells that mature recognize MHC molecules at a frequency as high as in CD4+ cells in normal mice. These results demonstrate that: 1) the germline sequences encoding TCRs are biased toward reactivity to MHC molecules; and 2) CD4+ cells as opposed to CD8+ cells have distinct lineage commitment signals. These results also suggest that signals originating from Ly-6 can promote or substitute for signals generated from TCR that are required for positive selection. Moreover, this animal model offers a system to study T cell development in the thymus that can provide insights into mechanisms of lineage commitment in developing T cells.

Authors+Show Affiliations

Department of Cellular Biology, University of Georgia, Athens 30602, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9647222

Citation

Henderson, S C., et al. "CD4+ T Cells Mature in the Absence of MHC Class I and Class II Expression in Ly-6A.2 Transgenic Mice." Journal of Immunology (Baltimore, Md. : 1950), vol. 161, no. 1, 1998, pp. 175-82.
Henderson SC, Berezovskaya A, English A, et al. CD4+ T cells mature in the absence of MHC class I and class II expression in Ly-6A.2 transgenic mice. J Immunol. 1998;161(1):175-82.
Henderson, S. C., Berezovskaya, A., English, A., Palliser, D., Rock, K. L., & Bamezai, A. (1998). CD4+ T cells mature in the absence of MHC class I and class II expression in Ly-6A.2 transgenic mice. Journal of Immunology (Baltimore, Md. : 1950), 161(1), 175-82.
Henderson SC, et al. CD4+ T Cells Mature in the Absence of MHC Class I and Class II Expression in Ly-6A.2 Transgenic Mice. J Immunol. 1998 Jul 1;161(1):175-82. PubMed PMID: 9647222.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD4+ T cells mature in the absence of MHC class I and class II expression in Ly-6A.2 transgenic mice. AU - Henderson,S C, AU - Berezovskaya,A, AU - English,A, AU - Palliser,D, AU - Rock,K L, AU - Bamezai,A, PY - 1998/7/1/pubmed PY - 1998/7/1/medline PY - 1998/7/1/entrez SP - 175 EP - 82 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 161 IS - 1 N2 - The TCRs expressed on T lymphocytes recognize foreign peptides bound to MHC molecules. This reactivity is the basis of specific immune response to the foreign Ag. How such specificities are generated in the thymus is still being debated. Signals generated through TCR upon interaction with self MHC-peptide complexes are critical for maturation of the CD4+ helper and CD8+ cytotoxic subsets. We have observed maturation of CD4+ but not CD8+ T cells in Ly-6A.2 transgenic MHC null mice. Since there can be no interactions with MHC molecules in these mice, these CD4+ cells must express the T cell repertoire that exists before positive and negative selection. Interestingly, despite an absence of selection by MHC molecules, the CD4+ cells that mature recognize MHC molecules at a frequency as high as in CD4+ cells in normal mice. These results demonstrate that: 1) the germline sequences encoding TCRs are biased toward reactivity to MHC molecules; and 2) CD4+ cells as opposed to CD8+ cells have distinct lineage commitment signals. These results also suggest that signals originating from Ly-6 can promote or substitute for signals generated from TCR that are required for positive selection. Moreover, this animal model offers a system to study T cell development in the thymus that can provide insights into mechanisms of lineage commitment in developing T cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9647222/CD4+_T_cells_mature_in_the_absence_of_MHC_class_I_and_class_II_expression_in_Ly_6A_2_transgenic_mice_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9647222 DB - PRIME DP - Unbound Medicine ER -