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Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers.
J Invertebr Pathol. 1998 Jul; 72(1):21-7.JI

Abstract

Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction technique (PCR) with BOX sequence-specific primers. Isolates from Argentina generated three groups of patterns (designated A, B, and C), while P. larvae subsp. larvae strains obtained from other countries yielded two distinguishable patterns (coincident with A and B). Strains from U.S. A. and Germany were identical and related to Group A, while all Czech and English isolates belonged to Group B. Strains from France, Poland, Italy, Sweden, and New Zealand showed two different patterns (A and B). Comparisons of the biochemical type and genotype of isolates rendered no obvious linkage between both features. These results suggest that AFB in Argentina resulted from multiple sources of contaminated material.

Authors+Show Affiliations

Facultad de Ciencias Agrarias y Forestales, U.N.L.P., calles 60 y 118, cc 31, La Plata, 1900, Argentina.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9647697

Citation

Alippi, A M., and O M. Aguilar. "Characterization of Isolates of Paenibacillus Larvae Subsp. Larvae From Diverse Geographical Origin By the Polymerase Chain Reaction and BOX Primers." Journal of Invertebrate Pathology, vol. 72, no. 1, 1998, pp. 21-7.
Alippi AM, Aguilar OM. Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers. J Invertebr Pathol. 1998;72(1):21-7.
Alippi, A. M., & Aguilar, O. M. (1998). Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers. Journal of Invertebrate Pathology, 72(1), 21-7.
Alippi AM, Aguilar OM. Characterization of Isolates of Paenibacillus Larvae Subsp. Larvae From Diverse Geographical Origin By the Polymerase Chain Reaction and BOX Primers. J Invertebr Pathol. 1998;72(1):21-7. PubMed PMID: 9647697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers. AU - Alippi,A M, AU - Aguilar,O M, PY - 1998/7/2/pubmed PY - 1998/7/2/medline PY - 1998/7/2/entrez SP - 21 EP - 7 JF - Journal of invertebrate pathology JO - J. Invertebr. Pathol. VL - 72 IS - 1 N2 - Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction technique (PCR) with BOX sequence-specific primers. Isolates from Argentina generated three groups of patterns (designated A, B, and C), while P. larvae subsp. larvae strains obtained from other countries yielded two distinguishable patterns (coincident with A and B). Strains from U.S. A. and Germany were identical and related to Group A, while all Czech and English isolates belonged to Group B. Strains from France, Poland, Italy, Sweden, and New Zealand showed two different patterns (A and B). Comparisons of the biochemical type and genotype of isolates rendered no obvious linkage between both features. These results suggest that AFB in Argentina resulted from multiple sources of contaminated material. SN - 0022-2011 UR - https://www.unboundmedicine.com/medline/citation/9647697/Characterization_of_isolates_of_Paenibacillus_larvae_subsp__larvae_from_diverse_geographical_origin_by_the_polymerase_chain_reaction_and_BOX_primers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(98)94748-6 DB - PRIME DP - Unbound Medicine ER -