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Appearance of contractile endothelin-B receptors in rat mesenteric arterial segments following organ culture.
Acta Physiol Scand. 1998 Jun; 163(2):121-9.AP

Abstract

The aim of this study was to examine how different procedures for organ culture affect the expression of contractile endothelin(ET)-B receptors in a branch of the rat mesenteric artery. In fresh segments, ET-1 and ET-3 induced similar strong contractions, ET-1 being 20-fold more potent, whereas neither of the selective ETB receptor agonists, sarafotoxin 6c (S6c) nor IRL 1620, induced significant contractions. In segments cultured for 1 day, ET-3 was only 3-fold less potent as ET-1, and S6c and IRL 1620 induced concentration-dependent contractions which were about 60% of the ET-1 induced contraction. The maximum contractile response to S6c was not altered in segments cultured with foetal calf serum or in buffer solution, but was reduced to about 20% of the control value when cultured in glucose-free buffer solution. The contraction to S6c was abolished in segments placed in cold (4 degrees C) buffer solution. Removal of the endothelium had no effect on the S6c-induced contractions. Arteries cultured at isometric tension (at 2 mN) for 1 day achieved the same contractile response for ETB agonists as resting segments. Pressurized arteries (60 mmHg) did not constrict to S6c when mounted as a fresh segment but demonstrated a strong contraction after 1 day at this transmural pressure. This study suggests that the appearance of ETB receptor mediated contraction following organ culture is not dependent on specific nutrients, endothelial factors or absence of intrinsic tension, but is a metabolically active process.

Authors+Show Affiliations

Department of Internal Medicine, Lund University Hospital, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9648630

Citation

Adner, M, et al. "Appearance of Contractile endothelin-B Receptors in Rat Mesenteric Arterial Segments Following Organ Culture." Acta Physiologica Scandinavica, vol. 163, no. 2, 1998, pp. 121-9.
Adner M, Geary GG, Edvinsson L. Appearance of contractile endothelin-B receptors in rat mesenteric arterial segments following organ culture. Acta Physiol Scand. 1998;163(2):121-9.
Adner, M., Geary, G. G., & Edvinsson, L. (1998). Appearance of contractile endothelin-B receptors in rat mesenteric arterial segments following organ culture. Acta Physiologica Scandinavica, 163(2), 121-9.
Adner M, Geary GG, Edvinsson L. Appearance of Contractile endothelin-B Receptors in Rat Mesenteric Arterial Segments Following Organ Culture. Acta Physiol Scand. 1998;163(2):121-9. PubMed PMID: 9648630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Appearance of contractile endothelin-B receptors in rat mesenteric arterial segments following organ culture. AU - Adner,M, AU - Geary,G G, AU - Edvinsson,L, PY - 1998/7/2/pubmed PY - 1998/7/2/medline PY - 1998/7/2/entrez SP - 121 EP - 9 JF - Acta physiologica Scandinavica JO - Acta Physiol Scand VL - 163 IS - 2 N2 - The aim of this study was to examine how different procedures for organ culture affect the expression of contractile endothelin(ET)-B receptors in a branch of the rat mesenteric artery. In fresh segments, ET-1 and ET-3 induced similar strong contractions, ET-1 being 20-fold more potent, whereas neither of the selective ETB receptor agonists, sarafotoxin 6c (S6c) nor IRL 1620, induced significant contractions. In segments cultured for 1 day, ET-3 was only 3-fold less potent as ET-1, and S6c and IRL 1620 induced concentration-dependent contractions which were about 60% of the ET-1 induced contraction. The maximum contractile response to S6c was not altered in segments cultured with foetal calf serum or in buffer solution, but was reduced to about 20% of the control value when cultured in glucose-free buffer solution. The contraction to S6c was abolished in segments placed in cold (4 degrees C) buffer solution. Removal of the endothelium had no effect on the S6c-induced contractions. Arteries cultured at isometric tension (at 2 mN) for 1 day achieved the same contractile response for ETB agonists as resting segments. Pressurized arteries (60 mmHg) did not constrict to S6c when mounted as a fresh segment but demonstrated a strong contraction after 1 day at this transmural pressure. This study suggests that the appearance of ETB receptor mediated contraction following organ culture is not dependent on specific nutrients, endothelial factors or absence of intrinsic tension, but is a metabolically active process. SN - 0001-6772 UR - https://www.unboundmedicine.com/medline/citation/9648630/Appearance_of_contractile_endothelin_B_receptors_in_rat_mesenteric_arterial_segments_following_organ_culture_ L2 - https://doi.org/10.1046/j.1365-201X.1998.00369.x DB - PRIME DP - Unbound Medicine ER -