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Comparison of three fatty meals in healthy normolipidaemic men: high post-prandial retinyl ester response to soybean oil.
Eur J Clin Invest 1998; 28(5):407-15EJ

Abstract

BACKGROUND

Oral fat tolerance tests (FTTs) have been widely used as a tool to investigate post-prandial lipaemia. However, there is no consensus regarding the type and amount of fat used in the tests.

METHODS

We compared three commonly used FTTs, each containing 63 g of fat: a mixed meal, a liquid cream meal and a liquid soybean oil meal. The study group consisted of 10 healthy normolipidaemic men. We measured triglycerides (TGs), retinyl esters (REs), apolipoprotein E (apoE), apolipoprotein B-48 (apoB-48) and apolipoprotein B-100 (apoB-100) in plasma and in triglyceride-rich lipoprotein (TRL) fractions separated by density-gradient ultracentrifugation at baseline and 3, 4, 6, and 8 h after the FTTs.

RESULTS

We observed similar TGs, apoE, apoB-48 and apoB-100 responses after all three FTTs, despite the different fatty acid composition of the meals. In contrast, the commonly used marker for exogenous particles, RE, differed clearly when polyunsaturated (soybean oil) and saturated fat (cream or mixed meal) were used. The RE response in plasma (P < 0.005, repeated measures ANOVA), in chylomicrons (P < 0.013) and in very low-density lipoprotein (VLDL) 1 (P < 0.017), as well as the RE area under the incremental curve in plasma and chylomicron fractions, were markedly increased after the soybean oil meal compared with the mixed meal and cream meal tests. The peak of RE response occurred parallel to the responses of other markers (i.e. TG or apoB-48) of post-prandial TRL during soybean oil meal. In contrast, RE peak concentration was delayed after saturated fat-containing meals. After soybean oil, FTT plasma cholesterol concentration was lower and the chylomicron cholesterol concentration was higher compared with mixed or cream meals, but no differences were detected in post-prandial high-density lipoprotein (HDL)-cholesterol concentration.

CONCLUSION

When the amount of fat is similar, post-prandial responses of TG, apoE, apoB-48, apoB-100 and HDL-cholesterol were comparable after different FTTs.

Authors+Show Affiliations

Department of Medicine, University of Helsinki, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9650015

Citation

Mero, N, et al. "Comparison of Three Fatty Meals in Healthy Normolipidaemic Men: High Post-prandial Retinyl Ester Response to Soybean Oil." European Journal of Clinical Investigation, vol. 28, no. 5, 1998, pp. 407-15.
Mero N, Syvänne M, Rosseneu M, et al. Comparison of three fatty meals in healthy normolipidaemic men: high post-prandial retinyl ester response to soybean oil. Eur J Clin Invest. 1998;28(5):407-15.
Mero, N., Syvänne, M., Rosseneu, M., Labeur, C., Hilden, H., & Taskinen, M. R. (1998). Comparison of three fatty meals in healthy normolipidaemic men: high post-prandial retinyl ester response to soybean oil. European Journal of Clinical Investigation, 28(5), pp. 407-15.
Mero N, et al. Comparison of Three Fatty Meals in Healthy Normolipidaemic Men: High Post-prandial Retinyl Ester Response to Soybean Oil. Eur J Clin Invest. 1998;28(5):407-15. PubMed PMID: 9650015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of three fatty meals in healthy normolipidaemic men: high post-prandial retinyl ester response to soybean oil. AU - Mero,N, AU - Syvänne,M, AU - Rosseneu,M, AU - Labeur,C, AU - Hilden,H, AU - Taskinen,M R, PY - 1998/7/3/pubmed PY - 1998/7/3/medline PY - 1998/7/3/entrez SP - 407 EP - 15 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 28 IS - 5 N2 - BACKGROUND: Oral fat tolerance tests (FTTs) have been widely used as a tool to investigate post-prandial lipaemia. However, there is no consensus regarding the type and amount of fat used in the tests. METHODS: We compared three commonly used FTTs, each containing 63 g of fat: a mixed meal, a liquid cream meal and a liquid soybean oil meal. The study group consisted of 10 healthy normolipidaemic men. We measured triglycerides (TGs), retinyl esters (REs), apolipoprotein E (apoE), apolipoprotein B-48 (apoB-48) and apolipoprotein B-100 (apoB-100) in plasma and in triglyceride-rich lipoprotein (TRL) fractions separated by density-gradient ultracentrifugation at baseline and 3, 4, 6, and 8 h after the FTTs. RESULTS: We observed similar TGs, apoE, apoB-48 and apoB-100 responses after all three FTTs, despite the different fatty acid composition of the meals. In contrast, the commonly used marker for exogenous particles, RE, differed clearly when polyunsaturated (soybean oil) and saturated fat (cream or mixed meal) were used. The RE response in plasma (P < 0.005, repeated measures ANOVA), in chylomicrons (P < 0.013) and in very low-density lipoprotein (VLDL) 1 (P < 0.017), as well as the RE area under the incremental curve in plasma and chylomicron fractions, were markedly increased after the soybean oil meal compared with the mixed meal and cream meal tests. The peak of RE response occurred parallel to the responses of other markers (i.e. TG or apoB-48) of post-prandial TRL during soybean oil meal. In contrast, RE peak concentration was delayed after saturated fat-containing meals. After soybean oil, FTT plasma cholesterol concentration was lower and the chylomicron cholesterol concentration was higher compared with mixed or cream meals, but no differences were detected in post-prandial high-density lipoprotein (HDL)-cholesterol concentration. CONCLUSION: When the amount of fat is similar, post-prandial responses of TG, apoE, apoB-48, apoB-100 and HDL-cholesterol were comparable after different FTTs. SN - 0014-2972 UR - https://www.unboundmedicine.com/medline/citation/9650015/Comparison_of_three_fatty_meals_in_healthy_normolipidaemic_men:_high_post_prandial_retinyl_ester_response_to_soybean_oil_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0014-2972&amp;date=1998&amp;volume=28&amp;issue=5&amp;spage=407 DB - PRIME DP - Unbound Medicine ER -