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Determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine by high-performance liquid chromatography with tandem mass spectrometric detection.
J Chromatogr B Biomed Sci Appl 1998; 708(1-2):103-12JC

Abstract

A method is described for the determination of pilocarpine and its degradation products isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine. The method is based on a simple sample preparation step -- ultrafiltration for plasma and dilution for urine samples -- followed by a reversed-phase liquid chromatographic separation of the analytes and detection by means of tandem mass spectrometry. Parameters affecting the performance of these steps are discussed. The high sensitivity and selectivity of the method allow low ng/ml concentrations to be determined for all compounds in plasma and undiluted urine, which enables the investigation of the metabolic fate and elimination of pilocarpine after oral administration to humans.

Authors+Show Affiliations

Pharma-Bio Research International, Zuidlaren, Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9653952

Citation

van de Merbel, N C., et al. "Determination of Pilocarpine, Isopilocarpine, Pilocarpic Acid and Isopilocarpic Acid in Human Plasma and Urine By High-performance Liquid Chromatography With Tandem Mass Spectrometric Detection." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 708, no. 1-2, 1998, pp. 103-12.
van de Merbel NC, Tinke AP, Oosterhuis B, et al. Determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine by high-performance liquid chromatography with tandem mass spectrometric detection. J Chromatogr B Biomed Sci Appl. 1998;708(1-2):103-12.
van de Merbel, N. C., Tinke, A. P., Oosterhuis, B., Jonkman, J. H., & Bohle, J. F. (1998). Determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine by high-performance liquid chromatography with tandem mass spectrometric detection. Journal of Chromatography. B, Biomedical Sciences and Applications, 708(1-2), pp. 103-12.
van de Merbel NC, et al. Determination of Pilocarpine, Isopilocarpine, Pilocarpic Acid and Isopilocarpic Acid in Human Plasma and Urine By High-performance Liquid Chromatography With Tandem Mass Spectrometric Detection. J Chromatogr B Biomed Sci Appl. 1998 Apr 24;708(1-2):103-12. PubMed PMID: 9653952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine by high-performance liquid chromatography with tandem mass spectrometric detection. AU - van de Merbel,N C, AU - Tinke,A P, AU - Oosterhuis,B, AU - Jonkman,J H, AU - Bohle,J F, PY - 1998/7/8/pubmed PY - 1998/7/8/medline PY - 1998/7/8/entrez SP - 103 EP - 12 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J. Chromatogr. B Biomed. Sci. Appl. VL - 708 IS - 1-2 N2 - A method is described for the determination of pilocarpine and its degradation products isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine. The method is based on a simple sample preparation step -- ultrafiltration for plasma and dilution for urine samples -- followed by a reversed-phase liquid chromatographic separation of the analytes and detection by means of tandem mass spectrometry. Parameters affecting the performance of these steps are discussed. The high sensitivity and selectivity of the method allow low ng/ml concentrations to be determined for all compounds in plasma and undiluted urine, which enables the investigation of the metabolic fate and elimination of pilocarpine after oral administration to humans. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/9653952/Determination_of_pilocarpine_isopilocarpine_pilocarpic_acid_and_isopilocarpic_acid_in_human_plasma_and_urine_by_high_performance_liquid_chromatography_with_tandem_mass_spectrometric_detection_ DB - PRIME DP - Unbound Medicine ER -