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Detection of Angiostrongylus malaysiensis circulating antigen using monoclonal antibody-based enzyme-linked immunosorbent assay (MAb-ELISA).
Southeast Asian J Trop Med Public Health. 1997; 28 Suppl 1:143-7.SA

Abstract

Three MAbs 1C4.2D8, 1C4.2C4 and 1C4.1F5 were produced using sonicated adult worm antigens of Angiostrongylus malaysiensis and they were found to be secreters of IgG1. The MAbs 1C4.2C4 and 1C4.2D8 were found to react with antigens of A. malaysiensis and cross-react with the closely related A. cantonensis but not with other helminths. A total of 108 human sera collected from Orang Asli (aborigenes) from Grik, in the State of Perak were tested for A. malaysiensis infection using the MAb-ELISA. MAb 1C4.1F5 and 25 (23%) were positive. Twenty of these positive samples were tested with the MAb 1C4.2D8 and none was found to be positive.

Authors+Show Affiliations

Ambu S
Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
Rain AN
No affiliation info available
Mak JW
No affiliation info available
Maslah D
No affiliation info available
Maidah S
No affiliation info available

MeSH

AngiostrongylusAnimalsAntibodies, HelminthAntibodies, MonoclonalAntibody SpecificityAntigens, HelminthContinental Population GroupsCross ReactionsEnzyme-Linked Immunosorbent AssayHumansMalaysiaOceanic Ancestry GroupStrongylida Infections

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9656366

Citation

Ambu, S, et al. "Detection of Angiostrongylus Malaysiensis Circulating Antigen Using Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay (MAb-ELISA)." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 28 Suppl 1, 1997, pp. 143-7.
Ambu S, Rain AN, Mak JW, et al. Detection of Angiostrongylus malaysiensis circulating antigen using monoclonal antibody-based enzyme-linked immunosorbent assay (MAb-ELISA). Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:143-7.
Ambu, S., Rain, A. N., Mak, J. W., Maslah, D., & Maidah, S. (1997). Detection of Angiostrongylus malaysiensis circulating antigen using monoclonal antibody-based enzyme-linked immunosorbent assay (MAb-ELISA). The Southeast Asian Journal of Tropical Medicine and Public Health, 28 Suppl 1, 143-7.
Ambu S, et al. Detection of Angiostrongylus Malaysiensis Circulating Antigen Using Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay (MAb-ELISA). Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:143-7. PubMed PMID: 9656366.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Angiostrongylus malaysiensis circulating antigen using monoclonal antibody-based enzyme-linked immunosorbent assay (MAb-ELISA). AU - Ambu,S, AU - Rain,A N, AU - Mak,J W, AU - Maslah,D, AU - Maidah,S, PY - 1997/1/1/pubmed PY - 1998/7/10/medline PY - 1997/1/1/entrez SP - 143 EP - 7 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 28 Suppl 1 N2 - Three MAbs 1C4.2D8, 1C4.2C4 and 1C4.1F5 were produced using sonicated adult worm antigens of Angiostrongylus malaysiensis and they were found to be secreters of IgG1. The MAbs 1C4.2C4 and 1C4.2D8 were found to react with antigens of A. malaysiensis and cross-react with the closely related A. cantonensis but not with other helminths. A total of 108 human sera collected from Orang Asli (aborigenes) from Grik, in the State of Perak were tested for A. malaysiensis infection using the MAb-ELISA. MAb 1C4.1F5 and 25 (23%) were positive. Twenty of these positive samples were tested with the MAb 1C4.2D8 and none was found to be positive. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/9656366/Detection_of_Angiostrongylus_malaysiensis_circulating_antigen_using_monoclonal_antibody_based_enzyme_linked_immunosorbent_assay__MAb_ELISA__ DB - PRIME DP - Unbound Medicine ER -