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RpoS (sigma-S) controls expression of rsmA, a global regulator of secondary metabolites, harpin, and extracellular proteins in Erwinia carotovora.
J Bacteriol. 1998 Jul; 180(14):3629-34.JB

Abstract

RpoS (sigma-S or sigma-38) controls a large array of genes that are expressed during stationary phase and under various stress conditions in Escherichia coli and other bacteria. We document here that plant pathogenic and epiphytic Erwinia species, such as E. amylovora; E. carotovora subsp. atroseptica, betavasculorum, and carotovora; E. chrysanthemi; E. herbicola; E. rhapontici; and E. stewartii, possess rpoS genes and produce the alternate sigma factor. We show that rpoS transcription in E. carotovora subsp. carotovora is driven from a major promoter which resides within the nlpD gene located upstream of rpoS as in E. coli. RpoS- E. carotovora subsp. carotovoa strain AC5061, constructed by marker exchange, is more sensitive to hydrogen peroxide, carbon starvation, and acidic pH than its RpoS+ parent strain, AC5006. The basal levels of extracellular pectate lyase, polygalacturonase, and cellulase as well as those of transcripts of E. carotovora subsp. carotovora hrpN (hrpNEcc), the gene for the elicitor of the hypersensitive reaction, are higher in the RpoS- strain than in the RpoS+ parent. Likewise, compared to AC5006, AC5061 causes more extensive maceration of celery petioles. Our findings with the RpoS- mutant and strains carrying multiple copies rpoS+ DNA reveal that rpoS positively controls rsmA expression. We also present evidence that supports the hypothesis that the RpoS effect on extracellular enzyme levels, hrpNEcc expression, and virulence manifests itself by the modulation of rsmA expression.

Authors+Show Affiliations

Department of Plant Pathology, University of Missouri, Columbia, Missouri 65211, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

9658007

Citation

Mukherjee, A, et al. "RpoS (sigma-S) Controls Expression of rsmA, a Global Regulator of Secondary Metabolites, Harpin, and Extracellular Proteins in Erwinia Carotovora." Journal of Bacteriology, vol. 180, no. 14, 1998, pp. 3629-34.
Mukherjee A, Cui Y, Ma W, et al. RpoS (sigma-S) controls expression of rsmA, a global regulator of secondary metabolites, harpin, and extracellular proteins in Erwinia carotovora. J Bacteriol. 1998;180(14):3629-34.
Mukherjee, A., Cui, Y., Ma, W., Liu, Y., Ishihama, A., Eisenstark, A., & Chatterjee, A. K. (1998). RpoS (sigma-S) controls expression of rsmA, a global regulator of secondary metabolites, harpin, and extracellular proteins in Erwinia carotovora. Journal of Bacteriology, 180(14), 3629-34.
Mukherjee A, et al. RpoS (sigma-S) Controls Expression of rsmA, a Global Regulator of Secondary Metabolites, Harpin, and Extracellular Proteins in Erwinia Carotovora. J Bacteriol. 1998;180(14):3629-34. PubMed PMID: 9658007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RpoS (sigma-S) controls expression of rsmA, a global regulator of secondary metabolites, harpin, and extracellular proteins in Erwinia carotovora. AU - Mukherjee,A, AU - Cui,Y, AU - Ma,W, AU - Liu,Y, AU - Ishihama,A, AU - Eisenstark,A, AU - Chatterjee,A K, PY - 1998/7/11/pubmed PY - 1998/7/11/medline PY - 1998/7/11/entrez SP - 3629 EP - 34 JF - Journal of bacteriology JO - J Bacteriol VL - 180 IS - 14 N2 - RpoS (sigma-S or sigma-38) controls a large array of genes that are expressed during stationary phase and under various stress conditions in Escherichia coli and other bacteria. We document here that plant pathogenic and epiphytic Erwinia species, such as E. amylovora; E. carotovora subsp. atroseptica, betavasculorum, and carotovora; E. chrysanthemi; E. herbicola; E. rhapontici; and E. stewartii, possess rpoS genes and produce the alternate sigma factor. We show that rpoS transcription in E. carotovora subsp. carotovora is driven from a major promoter which resides within the nlpD gene located upstream of rpoS as in E. coli. RpoS- E. carotovora subsp. carotovoa strain AC5061, constructed by marker exchange, is more sensitive to hydrogen peroxide, carbon starvation, and acidic pH than its RpoS+ parent strain, AC5006. The basal levels of extracellular pectate lyase, polygalacturonase, and cellulase as well as those of transcripts of E. carotovora subsp. carotovora hrpN (hrpNEcc), the gene for the elicitor of the hypersensitive reaction, are higher in the RpoS- strain than in the RpoS+ parent. Likewise, compared to AC5006, AC5061 causes more extensive maceration of celery petioles. Our findings with the RpoS- mutant and strains carrying multiple copies rpoS+ DNA reveal that rpoS positively controls rsmA expression. We also present evidence that supports the hypothesis that the RpoS effect on extracellular enzyme levels, hrpNEcc expression, and virulence manifests itself by the modulation of rsmA expression. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/9658007/RpoS__sigma_S__controls_expression_of_rsmA_a_global_regulator_of_secondary_metabolites_harpin_and_extracellular_proteins_in_Erwinia_carotovora_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=9658007 DB - PRIME DP - Unbound Medicine ER -