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Detection of Ki-ras and p53 gene mutations in tissue and pancreatic juice from pancreatic adenocarcinomas.
J Gastroenterol 1998; 33(3):390-6JG

Abstract

Pancreatic carcinomas have a high incidence of Ki-ras mutations, and the genetic change is thought to occur at an early stage in the carcinogenesis. The aim of this study was to evaluate the usefulness of detecting genetic mutations in pure pancreatic juice (PPJ). DNA was extracted from tissue specimens of pancreatic carcinomas and from cells in PPJ, and subjected to polymerase chain reaction-single-strand conformation polymorphism analysis. Two types of mobility shifts that indicate Ki-ras mutations were observed in 13 of the 20 (65%) tissue specimens obtained by operation or autopsy. Ten of 15 specimens (67%) of PPJ collected from patients with pancreatic carcinomas showed two types of mobility shifts. Conventional imaging techniques did not show two in 10 of these patients. PPJ from patients with non-cancerous pancreatic diseases showed no Ki-ras mutations. The p53 tumor suppressor gene, examined by PCR-SSCP analysis, was mutated in 8 of the 20 tissue specimens obtained by operation or autopsy (40%). The detection of Ki-ras and p53 mutations in PPJ could be useful for the early diagnosis of pancreatic carcinomas, especially for neoplastic lesions of the intraductal type.

Authors+Show Affiliations

First Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9658319

Citation

Kondoh, S, et al. "Detection of Ki-ras and P53 Gene Mutations in Tissue and Pancreatic Juice From Pancreatic Adenocarcinomas." Journal of Gastroenterology, vol. 33, no. 3, 1998, pp. 390-6.
Kondoh S, Kaino M, Okita S, et al. Detection of Ki-ras and p53 gene mutations in tissue and pancreatic juice from pancreatic adenocarcinomas. J Gastroenterol. 1998;33(3):390-6.
Kondoh, S., Kaino, M., Okita, S., Ryozawa, S., Akiyama, T., & Okita, K. (1998). Detection of Ki-ras and p53 gene mutations in tissue and pancreatic juice from pancreatic adenocarcinomas. Journal of Gastroenterology, 33(3), pp. 390-6.
Kondoh S, et al. Detection of Ki-ras and P53 Gene Mutations in Tissue and Pancreatic Juice From Pancreatic Adenocarcinomas. J Gastroenterol. 1998;33(3):390-6. PubMed PMID: 9658319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Ki-ras and p53 gene mutations in tissue and pancreatic juice from pancreatic adenocarcinomas. AU - Kondoh,S, AU - Kaino,M, AU - Okita,S, AU - Ryozawa,S, AU - Akiyama,T, AU - Okita,K, PY - 1998/7/11/pubmed PY - 1998/7/11/medline PY - 1998/7/11/entrez SP - 390 EP - 6 JF - Journal of gastroenterology JO - J. Gastroenterol. VL - 33 IS - 3 N2 - Pancreatic carcinomas have a high incidence of Ki-ras mutations, and the genetic change is thought to occur at an early stage in the carcinogenesis. The aim of this study was to evaluate the usefulness of detecting genetic mutations in pure pancreatic juice (PPJ). DNA was extracted from tissue specimens of pancreatic carcinomas and from cells in PPJ, and subjected to polymerase chain reaction-single-strand conformation polymorphism analysis. Two types of mobility shifts that indicate Ki-ras mutations were observed in 13 of the 20 (65%) tissue specimens obtained by operation or autopsy. Ten of 15 specimens (67%) of PPJ collected from patients with pancreatic carcinomas showed two types of mobility shifts. Conventional imaging techniques did not show two in 10 of these patients. PPJ from patients with non-cancerous pancreatic diseases showed no Ki-ras mutations. The p53 tumor suppressor gene, examined by PCR-SSCP analysis, was mutated in 8 of the 20 tissue specimens obtained by operation or autopsy (40%). The detection of Ki-ras and p53 mutations in PPJ could be useful for the early diagnosis of pancreatic carcinomas, especially for neoplastic lesions of the intraductal type. SN - 0944-1174 UR - https://www.unboundmedicine.com/medline/citation/9658319/Detection_of_Ki_ras_and_p53_gene_mutations_in_tissue_and_pancreatic_juice_from_pancreatic_adenocarcinomas_ L2 - https://medlineplus.gov/pancreaticcancer.html DB - PRIME DP - Unbound Medicine ER -