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Cloning and characterization of a CMCase gene, celB, of Erwinia carotovora subsp. carotovora LY34 and its comparison to celA.
Mol Cells. 1998 Jun 30; 8(3):280-5.MC

Abstract

The phytopathogenic Erwinia carotovora subsp. carotovora (Ecc) LY34 secretes multiple isozymes of the plant cell wall-disintegrating enzyme endoglucanase. We isolated a second cel gene encoding CMCase in Ecc LY34. A 3.7 kb fragment was subcloned to generate pLYCB100 (celB). The structural organization of the celB gene consists of an open reading frame (ORF) of 792 bp encoding 264 264 amino acid residues with a calculated molecular weight of 29,890 Da The predicted amino acid sequence of CelB is very similar to that of CelS of another Ecc strain, SCRI193, but completely different from that of the CelA of Ecc LY34. It belongs to the glycosyl hydrolase family 12 based on amino acid sequence similarities. It is optimally active at pH 6.8 with an optimal temperature of about 50 degrees C. The CMCase activity of CelB is about 60% of that of CelA.

Authors+Show Affiliations

Genetic Engineering Institute, Gyeongsang National University, Chinju, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9666464

Citation

Woo Park, Y, et al. "Cloning and Characterization of a CMCase Gene, celB, of Erwinia Carotovora Subsp. Carotovora LY34 and Its Comparison to CelA." Molecules and Cells, vol. 8, no. 3, 1998, pp. 280-5.
Woo Park Y, Tech Lim S, Dae Yun H. Cloning and characterization of a CMCase gene, celB, of Erwinia carotovora subsp. carotovora LY34 and its comparison to celA. Mol Cells. 1998;8(3):280-5.
Woo Park, Y., Tech Lim, S., & Dae Yun, H. (1998). Cloning and characterization of a CMCase gene, celB, of Erwinia carotovora subsp. carotovora LY34 and its comparison to celA. Molecules and Cells, 8(3), 280-5.
Woo Park Y, Tech Lim S, Dae Yun H. Cloning and Characterization of a CMCase Gene, celB, of Erwinia Carotovora Subsp. Carotovora LY34 and Its Comparison to CelA. Mol Cells. 1998 Jun 30;8(3):280-5. PubMed PMID: 9666464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of a CMCase gene, celB, of Erwinia carotovora subsp. carotovora LY34 and its comparison to celA. AU - Woo Park,Y, AU - Tech Lim,S, AU - Dae Yun,H, PY - 1998/7/17/pubmed PY - 1998/7/17/medline PY - 1998/7/17/entrez SP - 280 EP - 5 JF - Molecules and cells JO - Mol Cells VL - 8 IS - 3 N2 - The phytopathogenic Erwinia carotovora subsp. carotovora (Ecc) LY34 secretes multiple isozymes of the plant cell wall-disintegrating enzyme endoglucanase. We isolated a second cel gene encoding CMCase in Ecc LY34. A 3.7 kb fragment was subcloned to generate pLYCB100 (celB). The structural organization of the celB gene consists of an open reading frame (ORF) of 792 bp encoding 264 264 amino acid residues with a calculated molecular weight of 29,890 Da The predicted amino acid sequence of CelB is very similar to that of CelS of another Ecc strain, SCRI193, but completely different from that of the CelA of Ecc LY34. It belongs to the glycosyl hydrolase family 12 based on amino acid sequence similarities. It is optimally active at pH 6.8 with an optimal temperature of about 50 degrees C. The CMCase activity of CelB is about 60% of that of CelA. SN - 1016-8478 UR - https://www.unboundmedicine.com/medline/citation/9666464/Cloning_and_characterization_of_a_CMCase_gene_celB_of_Erwinia_carotovora_subsp__carotovora_LY34_and_its_comparison_to_celA_ L2 - http://www.molcells.org/journal/view.html?year=1998&volume=8&number=3&spage=280 DB - PRIME DP - Unbound Medicine ER -