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Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora.
Mol Plant Microbe Interact. 1998 Aug; 11(8):743-52.MP

Abstract

Production of extracellular, plant cell wall degrading enzymes, the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, is coordinately controlled by a complex regulatory network. Insertion mutants in the exp (extracellular enzyme production) loci exhibit pleiotropic defects in virulence and the growth-phase-dependent transcriptional activation of genes encoding extracellular enzymes. Two new exp mutations, designated expA and expS, were characterized. Introduction of the corresponding wild-type alleles to the mutants complemented both the lack of virulence and the impaired production of plant cell wall degrading enzymes. The expA gene was shown to encode a 24-kDa polypeptide that is structurally and functionally related to the uvrY gene product of Escherichia coli and the GacA response regulator of Pseudomonas fluorescens. Functional similarity of expA and uvrY was demonstrated by genetic complementation. The expA gene is organized in an operon together with a uvrC-like gene, identical to the organization of uvrY and uvrC in E. coli. The unlinked expS gene encodes a putative sensor kinase that shows 92% identity to the recently described rpfA gene product from another E. carotovora subsp. carotovora strain. Our data suggest that ExpS and ExpA are members of two-component sensor kinase and response regulator families, respectively. These two proteins might interact in controlling virulence gene expression in E. carotovora subsp. carotovora.

Authors+Show Affiliations

Department of Plant Biology, Uppsala Genetic Center, Swedish University of Agricultural Sciences, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9675890

Citation

Eriksson, A R., et al. "Two-component Regulators Involved in the Global Control of Virulence in Erwinia Carotovora Subsp. Carotovora." Molecular Plant-microbe Interactions : MPMI, vol. 11, no. 8, 1998, pp. 743-52.
Eriksson AR, Andersson RA, Pirhonen M, et al. Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora. Mol Plant Microbe Interact. 1998;11(8):743-52.
Eriksson, A. R., Andersson, R. A., Pirhonen, M., & Palva, E. T. (1998). Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora. Molecular Plant-microbe Interactions : MPMI, 11(8), 743-52.
Eriksson AR, et al. Two-component Regulators Involved in the Global Control of Virulence in Erwinia Carotovora Subsp. Carotovora. Mol Plant Microbe Interact. 1998;11(8):743-52. PubMed PMID: 9675890.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora. AU - Eriksson,A R, AU - Andersson,R A, AU - Pirhonen,M, AU - Palva,E T, PY - 1998/7/24/pubmed PY - 1998/7/24/medline PY - 1998/7/24/entrez SP - 743 EP - 52 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 11 IS - 8 N2 - Production of extracellular, plant cell wall degrading enzymes, the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, is coordinately controlled by a complex regulatory network. Insertion mutants in the exp (extracellular enzyme production) loci exhibit pleiotropic defects in virulence and the growth-phase-dependent transcriptional activation of genes encoding extracellular enzymes. Two new exp mutations, designated expA and expS, were characterized. Introduction of the corresponding wild-type alleles to the mutants complemented both the lack of virulence and the impaired production of plant cell wall degrading enzymes. The expA gene was shown to encode a 24-kDa polypeptide that is structurally and functionally related to the uvrY gene product of Escherichia coli and the GacA response regulator of Pseudomonas fluorescens. Functional similarity of expA and uvrY was demonstrated by genetic complementation. The expA gene is organized in an operon together with a uvrC-like gene, identical to the organization of uvrY and uvrC in E. coli. The unlinked expS gene encodes a putative sensor kinase that shows 92% identity to the recently described rpfA gene product from another E. carotovora subsp. carotovora strain. Our data suggest that ExpS and ExpA are members of two-component sensor kinase and response regulator families, respectively. These two proteins might interact in controlling virulence gene expression in E. carotovora subsp. carotovora. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/9675890/Two_component_regulators_involved_in_the_global_control_of_virulence_in_Erwinia_carotovora_subsp__carotovora_ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.8.743?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -