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gamma delta TCR+ intestinal intraepithelial lymphocytes (i-IEL) in reaction against intestinal nematode Trichinella spiralis.
Comp Immunol Microbiol Infect Dis. 1998 Jul; 21(3):201-14.CI

Abstract

To assess the gamma delta TCR T cells in the control of the timing of the mucosal response to enteric parasitic infections, we used C57BL mice, orally infected with 200 viable T. spiralis larvae. The small intestine, spleens and Peyer's patches (PP) were excised on 1, 4, 7, 14, 21 and 29 postinfection days (p.i.) for immunophenotyping and histological studies. Uninfected mice served as control. Characterization of isolated lymphocytes of C57BL control mice, confirmed that T cell immunophenotype differs in spleen, PP and i-IEL. Practically all i-IEL were CD3+ cells (83%). In addition, most of the i-IEL expressed Ly-2 (65%). Among the i-IEL, the level of gamma delta TCR+ cells was significantly higher (29%) than that found in spleen (3%) and PP (3%). The expression was high on CD3+ and Ly-2+ (26 and 21%, respectively) and low on L3T4+ i-IEL (< 1%). During T. spiralis infection alpha beta TCR+ CD3+, gamma delta TCR+ CD3+ and gamma delta TCR+ Ly-2+ i-IEL increased on day 4 and 7. However, infected mice displayed a reduction in i-IEL number from 14 to 29 p.i. day. At the same time the proportion of gamma delta TCR on spleen Ly-2+ and on PP CD3+ and Ly-2+ cells increased on 14 and 21 p.i. day. Adult worms were expelled from the gut by day 14. Thus, the kinetics of gamma delta TCR+ i-IEL, but not spleen and PP gamma delta TCR, corresponded to the kinetics of worm expulsion in C57BL mice. Most murine i-IEL of the gamma delta T cell lineage tend to be cytolytic when activated. We speculated that gamma delta T cells of i-IEL during the early stages of infection recognize and eliminate damaged epithelial cells generated by parasite antigens, simultaneously accelerating the worm expulsion.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Veterinary Faculty, University of Zagreb, Croatia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9681243

Citation

Bozić, F, et al. "Gamma Delta TCR+ Intestinal Intraepithelial Lymphocytes (i-IEL) in Reaction Against Intestinal Nematode Trichinella Spiralis." Comparative Immunology, Microbiology and Infectious Diseases, vol. 21, no. 3, 1998, pp. 201-14.
Bozić F, Forcić D, Mazuran R, et al. Gamma delta TCR+ intestinal intraepithelial lymphocytes (i-IEL) in reaction against intestinal nematode Trichinella spiralis. Comp Immunol Microbiol Infect Dis. 1998;21(3):201-14.
Bozić, F., Forcić, D., Mazuran, R., Marinculić, A., Kozarić, Z., & Stojcević, D. (1998). Gamma delta TCR+ intestinal intraepithelial lymphocytes (i-IEL) in reaction against intestinal nematode Trichinella spiralis. Comparative Immunology, Microbiology and Infectious Diseases, 21(3), 201-14.
Bozić F, et al. Gamma Delta TCR+ Intestinal Intraepithelial Lymphocytes (i-IEL) in Reaction Against Intestinal Nematode Trichinella Spiralis. Comp Immunol Microbiol Infect Dis. 1998;21(3):201-14. PubMed PMID: 9681243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - gamma delta TCR+ intestinal intraepithelial lymphocytes (i-IEL) in reaction against intestinal nematode Trichinella spiralis. AU - Bozić,F, AU - Forcić,D, AU - Mazuran,R, AU - Marinculić,A, AU - Kozarić,Z, AU - Stojcević,D, PY - 1998/7/29/pubmed PY - 1998/7/29/medline PY - 1998/7/29/entrez SP - 201 EP - 14 JF - Comparative immunology, microbiology and infectious diseases JO - Comp Immunol Microbiol Infect Dis VL - 21 IS - 3 N2 - To assess the gamma delta TCR T cells in the control of the timing of the mucosal response to enteric parasitic infections, we used C57BL mice, orally infected with 200 viable T. spiralis larvae. The small intestine, spleens and Peyer's patches (PP) were excised on 1, 4, 7, 14, 21 and 29 postinfection days (p.i.) for immunophenotyping and histological studies. Uninfected mice served as control. Characterization of isolated lymphocytes of C57BL control mice, confirmed that T cell immunophenotype differs in spleen, PP and i-IEL. Practically all i-IEL were CD3+ cells (83%). In addition, most of the i-IEL expressed Ly-2 (65%). Among the i-IEL, the level of gamma delta TCR+ cells was significantly higher (29%) than that found in spleen (3%) and PP (3%). The expression was high on CD3+ and Ly-2+ (26 and 21%, respectively) and low on L3T4+ i-IEL (< 1%). During T. spiralis infection alpha beta TCR+ CD3+, gamma delta TCR+ CD3+ and gamma delta TCR+ Ly-2+ i-IEL increased on day 4 and 7. However, infected mice displayed a reduction in i-IEL number from 14 to 29 p.i. day. At the same time the proportion of gamma delta TCR on spleen Ly-2+ and on PP CD3+ and Ly-2+ cells increased on 14 and 21 p.i. day. Adult worms were expelled from the gut by day 14. Thus, the kinetics of gamma delta TCR+ i-IEL, but not spleen and PP gamma delta TCR, corresponded to the kinetics of worm expulsion in C57BL mice. Most murine i-IEL of the gamma delta T cell lineage tend to be cytolytic when activated. We speculated that gamma delta T cells of i-IEL during the early stages of infection recognize and eliminate damaged epithelial cells generated by parasite antigens, simultaneously accelerating the worm expulsion. SN - 0147-9571 UR - https://www.unboundmedicine.com/medline/citation/9681243/gamma_delta_TCR+_intestinal_intraepithelial_lymphocytes__i_IEL__in_reaction_against_intestinal_nematode_Trichinella_spiralis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-9571(98)00014-9 DB - PRIME DP - Unbound Medicine ER -