Tags

Type your tag names separated by a space and hit enter

Tumor necrosis factor-alpha and soluble tumor necrosis factor receptors in the culture supernatants of polymorphonuclear cells and peripheral blood mononuclear cells from cancer patients.
Eur Cytokine Netw. 1998 Jun; 9(2):155-9.EC

Abstract

Recent clinical and experimental studies have focused on the measurement of cytokines and their regulators, produced by immunocompetent cells. Their estimation may be used as parameters for the immune potential of cancer patients. In the present study we studied the ability of unstimulated and lipopolysaccharide (LPS)-stimulated polymorphonuclear cells (PMN) and peripheral blood mononuclear cells (PBMC) from oral cavity cancer and breast cancer patients to release tumor necrosis factor alpha (TNF-alpha) and soluble tumor necrosis factor receptors (sTNFR). There were significant differences concerning the parameters examined for PMN and PBMC from cancer patients as compared with normal subjects. We found significantly higher concentrations of sTNF-R p75 than sTNF-R p55 in the cell-culture supernatants. The culture supernatants of cells from oral cavity cancer patients contained higher concentrations of TNF-alpha and lower concentrations of sTNF-R p55 and sTNF-R p75 in comparison with breast cancer cell supernatants. In contrast, cells from breast cancer patients secreted lower concentrations of TNF-alpha and higher concentrations of sTNF-R p55 and sTNF-R p75. Although PBMC secreted higher concentrations of mediators than PMN, the quantitative dominance of PMN in the peripheral blood suggests an essential role of these cells in the defense reactions controlled by TNF-alpha.

Links

FREE Publisher Full Text

Authors+Show Affiliations

Jablońska E
Department of Immunopathology, Medical Academy, Bialystok, Poland.
Kiluk M
No affiliation info available
Piotrowski L
No affiliation info available
Grabowska Z
No affiliation info available
Markiewicz W
No affiliation info available
Jablonski J
No affiliation info available

MeSH

AdultAgedAntigens, CDBreast NeoplasmsCase-Control StudiesCells, CulturedCulture MediaFemaleHumansLeukocytes, MononuclearMaleMiddle AgedMouth NeoplasmsNeutrophilsReceptors, Tumor Necrosis FactorReceptors, Tumor Necrosis Factor, Type IReceptors, Tumor Necrosis Factor, Type IISolubilityTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9681391

Citation

Jablońska, E, et al. "Tumor Necrosis Factor-alpha and Soluble Tumor Necrosis Factor Receptors in the Culture Supernatants of Polymorphonuclear Cells and Peripheral Blood Mononuclear Cells From Cancer Patients." European Cytokine Network, vol. 9, no. 2, 1998, pp. 155-9.
Jablońska E, Kiluk M, Piotrowski L, et al. Tumor necrosis factor-alpha and soluble tumor necrosis factor receptors in the culture supernatants of polymorphonuclear cells and peripheral blood mononuclear cells from cancer patients. Eur Cytokine Netw. 1998;9(2):155-9.
Jablońska, E., Kiluk, M., Piotrowski, L., Grabowska, Z., Markiewicz, W., & Jablonski, J. (1998). Tumor necrosis factor-alpha and soluble tumor necrosis factor receptors in the culture supernatants of polymorphonuclear cells and peripheral blood mononuclear cells from cancer patients. European Cytokine Network, 9(2), 155-9.
Jablońska E, et al. Tumor Necrosis Factor-alpha and Soluble Tumor Necrosis Factor Receptors in the Culture Supernatants of Polymorphonuclear Cells and Peripheral Blood Mononuclear Cells From Cancer Patients. Eur Cytokine Netw. 1998;9(2):155-9. PubMed PMID: 9681391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tumor necrosis factor-alpha and soluble tumor necrosis factor receptors in the culture supernatants of polymorphonuclear cells and peripheral blood mononuclear cells from cancer patients. AU - Jablońska,E, AU - Kiluk,M, AU - Piotrowski,L, AU - Grabowska,Z, AU - Markiewicz,W, AU - Jablonski,J, PY - 1998/7/29/pubmed PY - 1998/7/29/medline PY - 1998/7/29/entrez SP - 155 EP - 9 JF - European cytokine network JO - Eur Cytokine Netw VL - 9 IS - 2 N2 - Recent clinical and experimental studies have focused on the measurement of cytokines and their regulators, produced by immunocompetent cells. Their estimation may be used as parameters for the immune potential of cancer patients. In the present study we studied the ability of unstimulated and lipopolysaccharide (LPS)-stimulated polymorphonuclear cells (PMN) and peripheral blood mononuclear cells (PBMC) from oral cavity cancer and breast cancer patients to release tumor necrosis factor alpha (TNF-alpha) and soluble tumor necrosis factor receptors (sTNFR). There were significant differences concerning the parameters examined for PMN and PBMC from cancer patients as compared with normal subjects. We found significantly higher concentrations of sTNF-R p75 than sTNF-R p55 in the cell-culture supernatants. The culture supernatants of cells from oral cavity cancer patients contained higher concentrations of TNF-alpha and lower concentrations of sTNF-R p55 and sTNF-R p75 in comparison with breast cancer cell supernatants. In contrast, cells from breast cancer patients secreted lower concentrations of TNF-alpha and higher concentrations of sTNF-R p55 and sTNF-R p75. Although PBMC secreted higher concentrations of mediators than PMN, the quantitative dominance of PMN in the peripheral blood suggests an essential role of these cells in the defense reactions controlled by TNF-alpha. SN - 1148-5493 UR - https://www.unboundmedicine.com/medline/citation/9681391/Tumor_necrosis_factor_alpha_and_soluble_tumor_necrosis_factor_receptors_in_the_culture_supernatants_of_polymorphonuclear_cells_and_peripheral_blood_mononuclear_cells_from_cancer_patients_ L2 - http://www.jle.com/medline.md?issn=1148-5493&vol=9&iss=2&page=155 DB - PRIME DP - Unbound Medicine ER -