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Receptors in lateral hypothalamic area involved in insular cortex sympathetic responses.
Am J Physiol. 1998 08; 275(2):H689-96.AJ

Abstract

Previous evidence has shown that sympathetic nerve responses to insular cortical (IC) stimulation are mediated by synapses within the lateral hypothalamic area (LHA) and ventrolateral medulla. The present study determined the receptor(s) involved at the synapse in the LHA associated with stimulation-evoked IC sympathetic responses. Twenty-seven male Wistar rats were instrumented for renal nerve activity, arterial pressure, and heart rate recording. The right IC was stimulated with a bipolar electrode (200-1,000 microA, 2 ms, 0.8 Hz) resulting in sympathetic nerve responses. Antagonists were then pressure injected into the ipsilateral LHA (300-500 nl). Kynurenate (250 mM) injections resulted in 51 +/- 8% (range 0-100%) block of IC-stimulated sympathetic nerve responses. Similarly, the N-methyl-D-aspartic acid (NMDA)-receptor antagonist DL-2-amino-5-phosphonopentanoic acid (200 microM) resulted in an inhibition (82 +/- 8%; range 51-100%) of IC-stimulated sympathetic responses. Injection of the non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (200 microM) had no effect on IC sympathetic responses. Injection of antagonists to GABA, acetylcholine, and adrenergic receptors was also without effect. No antagonist injections had any effects on baseline sympathetic nerve discharge, arterial pressure, or heart rate. These results suggest that the IC autonomic efferents projecting to the LHA utilize NMDA glutamatergic receptors.

Authors+Show Affiliations

Department of Physiology, University of Western Ontario, London, Ontario, Canada N6A 5C1.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9683459

Citation

Butcher, K S., and D F. Cechetto. "Receptors in Lateral Hypothalamic Area Involved in Insular Cortex Sympathetic Responses." The American Journal of Physiology, vol. 275, no. 2, 1998, pp. H689-96.
Butcher KS, Cechetto DF. Receptors in lateral hypothalamic area involved in insular cortex sympathetic responses. Am J Physiol. 1998;275(2):H689-96.
Butcher, K. S., & Cechetto, D. F. (1998). Receptors in lateral hypothalamic area involved in insular cortex sympathetic responses. The American Journal of Physiology, 275(2), H689-96. https://doi.org/10.1152/ajpheart.1998.275.2.H689
Butcher KS, Cechetto DF. Receptors in Lateral Hypothalamic Area Involved in Insular Cortex Sympathetic Responses. Am J Physiol. 1998;275(2):H689-96. PubMed PMID: 9683459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Receptors in lateral hypothalamic area involved in insular cortex sympathetic responses. AU - Butcher,K S, AU - Cechetto,D F, PY - 1998/7/31/pubmed PY - 1998/7/31/medline PY - 1998/7/31/entrez SP - H689 EP - 96 JF - The American journal of physiology JO - Am. J. Physiol. VL - 275 IS - 2 N2 - Previous evidence has shown that sympathetic nerve responses to insular cortical (IC) stimulation are mediated by synapses within the lateral hypothalamic area (LHA) and ventrolateral medulla. The present study determined the receptor(s) involved at the synapse in the LHA associated with stimulation-evoked IC sympathetic responses. Twenty-seven male Wistar rats were instrumented for renal nerve activity, arterial pressure, and heart rate recording. The right IC was stimulated with a bipolar electrode (200-1,000 microA, 2 ms, 0.8 Hz) resulting in sympathetic nerve responses. Antagonists were then pressure injected into the ipsilateral LHA (300-500 nl). Kynurenate (250 mM) injections resulted in 51 +/- 8% (range 0-100%) block of IC-stimulated sympathetic nerve responses. Similarly, the N-methyl-D-aspartic acid (NMDA)-receptor antagonist DL-2-amino-5-phosphonopentanoic acid (200 microM) resulted in an inhibition (82 +/- 8%; range 51-100%) of IC-stimulated sympathetic responses. Injection of the non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (200 microM) had no effect on IC sympathetic responses. Injection of antagonists to GABA, acetylcholine, and adrenergic receptors was also without effect. No antagonist injections had any effects on baseline sympathetic nerve discharge, arterial pressure, or heart rate. These results suggest that the IC autonomic efferents projecting to the LHA utilize NMDA glutamatergic receptors. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/9683459/Receptors_in_lateral_hypothalamic_area_involved_in_insular_cortex_sympathetic_responses_ L2 - http://www.physiology.org/doi/full/10.1152/ajpheart.1998.275.2.H689?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -