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Functional localization of soluble guanylate cyclase in turtle retina: modulation of cGMP by nitric oxide donors.
Vis Neurosci. 1998 May-Jun; 15(3):485-98.VN

Abstract

The second messenger cyclic guanosine monophosphate (cGMP) plays a role in many aspects of retinal processing. cGMP-gated channels function in photoreceptors, Müller, bipolar, and ganglion cells; and cGMP can modulate gap-junction conductivity. In the inner retina, both particulate and soluble guanylate cyclases can elevate levels of cGMP. The soluble isoform of guanylate cyclase is activated by nitric oxide (NO). In turtle retina, nitric oxide synthase, the enzyme that synthesizes NO, has been previously localized in discrete amacrine cells, somata in the ganglion cell layer, and in many processes in the inner plexiform layer. However, there have been no studies localizing soluble guanylate cyclase in the turtle retina. To functionally localize soluble guanylate cyclase, we stimulated retinas with the NO donors (+/-)-S-nitroso-N-acetylpenicillamine or spermine (nitric oxide) adduct, and then used immunocytochemistry to localize increases in cGMP-like immunoreactivity (cGMP-LI). The cells containing soluble guanylate cyclase should show cell autonomous increases in cGMP-LI in response to stimulation with NO. NO-stimulated increases in cGMP-LI occurred in many distinct amacrine cell types, select bipolar cells, some somata in the ganglion cell layer, and in discrete bands of processes in the inner plexiform layer. The pattern of cGMP-LI demonstrated qualitative dose response differences to the NO donors. This is the first localization of soluble guanylate cyclase in specific retinal neurons in the turtle; and the first functional activation of soluble guanylate cyclase in the amacrine cells of any species. The broad neuronal distribution of NO-stimulated cGMP-LI suggests that the NO/soluble guanylate cyclase/cGMP cascade is involved at several levels of visual processing in the inner retina.

Authors+Show Affiliations

Department of Biology, Boston University, MA 02215, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9685201

Citation

Blute, T A., et al. "Functional Localization of Soluble Guanylate Cyclase in Turtle Retina: Modulation of cGMP By Nitric Oxide Donors." Visual Neuroscience, vol. 15, no. 3, 1998, pp. 485-98.
Blute TA, Velasco P, Eldred WD. Functional localization of soluble guanylate cyclase in turtle retina: modulation of cGMP by nitric oxide donors. Vis Neurosci. 1998;15(3):485-98.
Blute, T. A., Velasco, P., & Eldred, W. D. (1998). Functional localization of soluble guanylate cyclase in turtle retina: modulation of cGMP by nitric oxide donors. Visual Neuroscience, 15(3), 485-98.
Blute TA, Velasco P, Eldred WD. Functional Localization of Soluble Guanylate Cyclase in Turtle Retina: Modulation of cGMP By Nitric Oxide Donors. Vis Neurosci. 1998 May-Jun;15(3):485-98. PubMed PMID: 9685201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional localization of soluble guanylate cyclase in turtle retina: modulation of cGMP by nitric oxide donors. AU - Blute,T A, AU - Velasco,P, AU - Eldred,W D, PY - 1998/7/31/pubmed PY - 1998/7/31/medline PY - 1998/7/31/entrez SP - 485 EP - 98 JF - Visual neuroscience JO - Vis Neurosci VL - 15 IS - 3 N2 - The second messenger cyclic guanosine monophosphate (cGMP) plays a role in many aspects of retinal processing. cGMP-gated channels function in photoreceptors, Müller, bipolar, and ganglion cells; and cGMP can modulate gap-junction conductivity. In the inner retina, both particulate and soluble guanylate cyclases can elevate levels of cGMP. The soluble isoform of guanylate cyclase is activated by nitric oxide (NO). In turtle retina, nitric oxide synthase, the enzyme that synthesizes NO, has been previously localized in discrete amacrine cells, somata in the ganglion cell layer, and in many processes in the inner plexiform layer. However, there have been no studies localizing soluble guanylate cyclase in the turtle retina. To functionally localize soluble guanylate cyclase, we stimulated retinas with the NO donors (+/-)-S-nitroso-N-acetylpenicillamine or spermine (nitric oxide) adduct, and then used immunocytochemistry to localize increases in cGMP-like immunoreactivity (cGMP-LI). The cells containing soluble guanylate cyclase should show cell autonomous increases in cGMP-LI in response to stimulation with NO. NO-stimulated increases in cGMP-LI occurred in many distinct amacrine cell types, select bipolar cells, some somata in the ganglion cell layer, and in discrete bands of processes in the inner plexiform layer. The pattern of cGMP-LI demonstrated qualitative dose response differences to the NO donors. This is the first localization of soluble guanylate cyclase in specific retinal neurons in the turtle; and the first functional activation of soluble guanylate cyclase in the amacrine cells of any species. The broad neuronal distribution of NO-stimulated cGMP-LI suggests that the NO/soluble guanylate cyclase/cGMP cascade is involved at several levels of visual processing in the inner retina. SN - 0952-5238 UR - https://www.unboundmedicine.com/medline/citation/9685201/Functional_localization_of_soluble_guanylate_cyclase_in_turtle_retina:_modulation_of_cGMP_by_nitric_oxide_donors_ L2 - https://www.cambridge.org/core/product/identifier/S0952523898153075/type/journal_article DB - PRIME DP - Unbound Medicine ER -