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Simplified solid-phase membrane immunobead assay (MIA) with monoclonal anti-ciguatoxin antibody (MAb-CTX) for detection of ciguatoxin and related polyether toxins.
J Nat Toxins. 1998 Feb; 7(1):1-21.JN

Abstract

The development of a simplified and modified procedure for the assessment of ciguatoxin (CTX) and related polyethers from ciguateric contaminated fish tissues is presented in this study. The previous method, stick-enzyme immunoassay (S-EIA) used an organic correction fluid-coated bamboo paddle stick for the solid phase; this new procedure, membrane immunobead assay (MIA), uses a plastic stick with a synthetic membrane laminated onto one end. The membrane is hydrophobic and serves as the solid-phase receptor for the binding of methanol-extracted CTX or its related polyether lipids from fish tissues. Detection of the bound polyether toxin(s) on the membrane is carried out with colored polystyrene beads coated with monoclonal antibody to CTX (MAb-CTX). Intensity of the color on the membrane is proportional to the amount of toxic polyethers on the membrane. The MIA is compared with previous procedures developed for CTX detection (S-EIA and solid-phase immunobead assay, SPIA) using State of Hawaii Department of Health (DOH) implicated ciguateric fishes, and reef fishes from Hawaii and Kwajalein. The data presented show good correlation between the three test systems, especially with ciguatera implicated fish and toxic, routinely assessed fishes in the mouse toxicity (MT) bioassay. Variations between MT results and those of the S-EIA, SPIA, and MIA of routine fishes are generally attributable to diverse toxins present in the fish species examined. The MIA is a simple, rapid, sensitive, and specific detection method for CTX and its related polyethers, with no reported false negative results. The test is useful for field and personal use and can be adapted to the laboratory for large-scale screening of potentially ciguateric fishes.

Authors+Show Affiliations

Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9689599

Citation

Hokama, Y, et al. "Simplified Solid-phase Membrane Immunobead Assay (MIA) With Monoclonal Anti-ciguatoxin Antibody (MAb-CTX) for Detection of Ciguatoxin and Related Polyether Toxins." Journal of Natural Toxins, vol. 7, no. 1, 1998, pp. 1-21.
Hokama Y, Nishimura K, Takenaka W, et al. Simplified solid-phase membrane immunobead assay (MIA) with monoclonal anti-ciguatoxin antibody (MAb-CTX) for detection of ciguatoxin and related polyether toxins. J Nat Toxins. 1998;7(1):1-21.
Hokama, Y., Nishimura, K., Takenaka, W., & Ebesu, J. S. (1998). Simplified solid-phase membrane immunobead assay (MIA) with monoclonal anti-ciguatoxin antibody (MAb-CTX) for detection of ciguatoxin and related polyether toxins. Journal of Natural Toxins, 7(1), 1-21.
Hokama Y, et al. Simplified Solid-phase Membrane Immunobead Assay (MIA) With Monoclonal Anti-ciguatoxin Antibody (MAb-CTX) for Detection of Ciguatoxin and Related Polyether Toxins. J Nat Toxins. 1998;7(1):1-21. PubMed PMID: 9689599.
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TY - JOUR T1 - Simplified solid-phase membrane immunobead assay (MIA) with monoclonal anti-ciguatoxin antibody (MAb-CTX) for detection of ciguatoxin and related polyether toxins. AU - Hokama,Y, AU - Nishimura,K, AU - Takenaka,W, AU - Ebesu,J S, PY - 1998/8/5/pubmed PY - 1998/8/5/medline PY - 1998/8/5/entrez SP - 1 EP - 21 JF - Journal of natural toxins JO - J Nat Toxins VL - 7 IS - 1 N2 - The development of a simplified and modified procedure for the assessment of ciguatoxin (CTX) and related polyethers from ciguateric contaminated fish tissues is presented in this study. The previous method, stick-enzyme immunoassay (S-EIA) used an organic correction fluid-coated bamboo paddle stick for the solid phase; this new procedure, membrane immunobead assay (MIA), uses a plastic stick with a synthetic membrane laminated onto one end. The membrane is hydrophobic and serves as the solid-phase receptor for the binding of methanol-extracted CTX or its related polyether lipids from fish tissues. Detection of the bound polyether toxin(s) on the membrane is carried out with colored polystyrene beads coated with monoclonal antibody to CTX (MAb-CTX). Intensity of the color on the membrane is proportional to the amount of toxic polyethers on the membrane. The MIA is compared with previous procedures developed for CTX detection (S-EIA and solid-phase immunobead assay, SPIA) using State of Hawaii Department of Health (DOH) implicated ciguateric fishes, and reef fishes from Hawaii and Kwajalein. The data presented show good correlation between the three test systems, especially with ciguatera implicated fish and toxic, routinely assessed fishes in the mouse toxicity (MT) bioassay. Variations between MT results and those of the S-EIA, SPIA, and MIA of routine fishes are generally attributable to diverse toxins present in the fish species examined. The MIA is a simple, rapid, sensitive, and specific detection method for CTX and its related polyethers, with no reported false negative results. The test is useful for field and personal use and can be adapted to the laboratory for large-scale screening of potentially ciguateric fishes. SN - 1058-8108 UR - https://www.unboundmedicine.com/medline/citation/9689599/Simplified_solid_phase_membrane_immunobead_assay__MIA__with_monoclonal_anti_ciguatoxin_antibody__MAb_CTX__for_detection_of_ciguatoxin_and_related_polyether_toxins_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:9689599 DB - PRIME DP - Unbound Medicine ER -