TY - JOUR T1 - A microsatellite map of wheat. AU - Röder,M S, AU - Korzun,V, AU - Wendehake,K, AU - Plaschke,J, AU - Tixier,M H, AU - Leroy,P, AU - Ganal,M W, PY - 1998/8/5/pubmed PY - 1998/8/5/medline PY - 1998/8/5/entrez SP - 2007 EP - 23 JF - Genetics JO - Genetics VL - 149 IS - 4 N2 - Hexaploid bread wheat (Triticum aestivum L. em. Thell) is one of the world's most important crop plants and displays a very low level of intraspecific polymorphism. We report the development of highly polymorphic microsatellite markers using procedures optimized for the large wheat genome. The isolation of microsatellite-containing clones from hypomethylated regions of the wheat genome increased the proportion of useful markers almost twofold. The majority (80%) of primer sets developed are genome-specific and detect only a single locus in one of the three genomes of bread wheat (A, B, or D). Only 20% of the markers detect more than one locus. A total of 279 loci amplified by 230 primer sets were placed onto a genetic framework map composed of RFLPs previously mapped in the reference population of the International Triticeae Mapping Initiative (ITMI) Opata 85 x W7984. Sixty-five microsatellites were mapped at a LOD >2.5, and 214 microsatellites were assigned to the most likely intervals. Ninety-three loci were mapped to the A genome, 115 to the B genome, and 71 to the D genome. The markers are randomly distributed along the linkage map, with clustering in several centromeric regions. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/9691054/A_microsatellite_map_of_wheat_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1093/genetics/149.4.2007 DB - PRIME DP - Unbound Medicine ER -