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A microsatellite map of wheat.
Genetics. 1998 Aug; 149(4):2007-23.G

Abstract

Hexaploid bread wheat (Triticum aestivum L. em. Thell) is one of the world's most important crop plants and displays a very low level of intraspecific polymorphism. We report the development of highly polymorphic microsatellite markers using procedures optimized for the large wheat genome. The isolation of microsatellite-containing clones from hypomethylated regions of the wheat genome increased the proportion of useful markers almost twofold. The majority (80%) of primer sets developed are genome-specific and detect only a single locus in one of the three genomes of bread wheat (A, B, or D). Only 20% of the markers detect more than one locus. A total of 279 loci amplified by 230 primer sets were placed onto a genetic framework map composed of RFLPs previously mapped in the reference population of the International Triticeae Mapping Initiative (ITMI) Opata 85 x W7984. Sixty-five microsatellites were mapped at a LOD >2.5, and 214 microsatellites were assigned to the most likely intervals. Ninety-three loci were mapped to the A genome, 115 to the B genome, and 71 to the D genome. The markers are randomly distributed along the linkage map, with clustering in several centromeric regions.

Authors+Show Affiliations

Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), 06466 Gatersleben, Germany. roder@ipk-gatesleben.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9691054

Citation

Röder, M S., et al. "A Microsatellite Map of Wheat." Genetics, vol. 149, no. 4, 1998, pp. 2007-23.
Röder MS, Korzun V, Wendehake K, et al. A microsatellite map of wheat. Genetics. 1998;149(4):2007-23.
Röder, M. S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M. H., Leroy, P., & Ganal, M. W. (1998). A microsatellite map of wheat. Genetics, 149(4), 2007-23.
Röder MS, et al. A Microsatellite Map of Wheat. Genetics. 1998;149(4):2007-23. PubMed PMID: 9691054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A microsatellite map of wheat. AU - Röder,M S, AU - Korzun,V, AU - Wendehake,K, AU - Plaschke,J, AU - Tixier,M H, AU - Leroy,P, AU - Ganal,M W, PY - 1998/8/5/pubmed PY - 1998/8/5/medline PY - 1998/8/5/entrez SP - 2007 EP - 23 JF - Genetics JO - Genetics VL - 149 IS - 4 N2 - Hexaploid bread wheat (Triticum aestivum L. em. Thell) is one of the world's most important crop plants and displays a very low level of intraspecific polymorphism. We report the development of highly polymorphic microsatellite markers using procedures optimized for the large wheat genome. The isolation of microsatellite-containing clones from hypomethylated regions of the wheat genome increased the proportion of useful markers almost twofold. The majority (80%) of primer sets developed are genome-specific and detect only a single locus in one of the three genomes of bread wheat (A, B, or D). Only 20% of the markers detect more than one locus. A total of 279 loci amplified by 230 primer sets were placed onto a genetic framework map composed of RFLPs previously mapped in the reference population of the International Triticeae Mapping Initiative (ITMI) Opata 85 x W7984. Sixty-five microsatellites were mapped at a LOD >2.5, and 214 microsatellites were assigned to the most likely intervals. Ninety-three loci were mapped to the A genome, 115 to the B genome, and 71 to the D genome. The markers are randomly distributed along the linkage map, with clustering in several centromeric regions. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/9691054/A_microsatellite_map_of_wheat_ L2 - http://www.genetics.org/cgi/pmidlookup?view=long&pmid=9691054 DB - PRIME DP - Unbound Medicine ER -