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Simple and sensitive high-performance liquid chromatographic determination of methamphetamines in human urine as derivatives of 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride, a new fluorescence derivatization reagent.
J Chromatogr B Biomed Sci Appl. 1998 Aug 07; 712(1-2):105-12.JC

Abstract

A simple, rapid and highly sensitive high-performance liquid chromatographic method for the determination of methamphetamine and its metabolites in human urine was developed. Without tedious pretreatment procedures, aliquots of methamphetamine spiked or abusers' urine samples were simply acidified, dried under a stream of N2 gas, and then derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride in acetonitrile in the presence of traces of triethylamine. Five derivatives were isocratically separated within 33 min by an ODS column, and the effluent was monitored at 440 nm (lambda(ex), 330 nm). Using 1-phenylethylamine as an internal standard calibration curves were confirmed to be linear up to at least 2x10(-5) M in urine with correlation coefficients of 0.998-1.000, while the detection limits at S/N=3 were 0.6-5.2 fmol per 5-microl injection. The relative standard deviations (n=5) of inter- and intra-day variations were less than 8.9%. The correlation between the concentrations of methamphetamine and amphetamine in urine determined by the proposed method and another currently accepted one was satisfactory. The method was successfully applied to urine samples collected from methamphetamine addicts.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Nagasaki University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9698233

Citation

Al-Dirbashi, O, et al. "Simple and Sensitive High-performance Liquid Chromatographic Determination of Methamphetamines in Human Urine as Derivatives of 4-(4,5-diphenyl-1H-imidazol-2-yl) Benzoyl Chloride, a New Fluorescence Derivatization Reagent." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 712, no. 1-2, 1998, pp. 105-12.
Al-Dirbashi O, Qvarnstrom J, Irgum K, et al. Simple and sensitive high-performance liquid chromatographic determination of methamphetamines in human urine as derivatives of 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride, a new fluorescence derivatization reagent. J Chromatogr B Biomed Sci Appl. 1998;712(1-2):105-12.
Al-Dirbashi, O., Qvarnstrom, J., Irgum, K., & Nakashima, K. (1998). Simple and sensitive high-performance liquid chromatographic determination of methamphetamines in human urine as derivatives of 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride, a new fluorescence derivatization reagent. Journal of Chromatography. B, Biomedical Sciences and Applications, 712(1-2), 105-12.
Al-Dirbashi O, et al. Simple and Sensitive High-performance Liquid Chromatographic Determination of Methamphetamines in Human Urine as Derivatives of 4-(4,5-diphenyl-1H-imidazol-2-yl) Benzoyl Chloride, a New Fluorescence Derivatization Reagent. J Chromatogr B Biomed Sci Appl. 1998 Aug 7;712(1-2):105-12. PubMed PMID: 9698233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple and sensitive high-performance liquid chromatographic determination of methamphetamines in human urine as derivatives of 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride, a new fluorescence derivatization reagent. AU - Al-Dirbashi,O, AU - Qvarnstrom,J, AU - Irgum,K, AU - Nakashima,K, PY - 1998/8/11/pubmed PY - 1998/8/11/medline PY - 1998/8/11/entrez SP - 105 EP - 12 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 712 IS - 1-2 N2 - A simple, rapid and highly sensitive high-performance liquid chromatographic method for the determination of methamphetamine and its metabolites in human urine was developed. Without tedious pretreatment procedures, aliquots of methamphetamine spiked or abusers' urine samples were simply acidified, dried under a stream of N2 gas, and then derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride in acetonitrile in the presence of traces of triethylamine. Five derivatives were isocratically separated within 33 min by an ODS column, and the effluent was monitored at 440 nm (lambda(ex), 330 nm). Using 1-phenylethylamine as an internal standard calibration curves were confirmed to be linear up to at least 2x10(-5) M in urine with correlation coefficients of 0.998-1.000, while the detection limits at S/N=3 were 0.6-5.2 fmol per 5-microl injection. The relative standard deviations (n=5) of inter- and intra-day variations were less than 8.9%. The correlation between the concentrations of methamphetamine and amphetamine in urine determined by the proposed method and another currently accepted one was satisfactory. The method was successfully applied to urine samples collected from methamphetamine addicts. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/9698233/Simple_and_sensitive_high_performance_liquid_chromatographic_determination_of_methamphetamines_in_human_urine_as_derivatives_of_4__45_diphenyl_1H_imidazol_2_yl__benzoyl_chloride_a_new_fluorescence_derivatization_reagent_ DB - PRIME DP - Unbound Medicine ER -