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Epidermal growth factor augments adaptation following small bowel resection: optimal dosage, route, and timing of administration.
J Surg Res. 1998 Jun; 77(1):11-6.JS

Abstract

BACKGROUND

In assorted animal models of small bowel resection (SBR), exogenous epidermal growth factor (EGF) has been shown to augment intestinal adaptation. This study was designed to elucidate the optimal dose, route, and timing of exogenous EGF to boost adaptation in our murine model of SBR.

METHODS

Male ICR mice underwent either 50% proximal SBR or sham surgery (bowel transection with reanastomosis) and then randomized to receive either saline or human recombinant EGF (5, 50, 150, or 300 microg/kg/day) by twice daily intraperitoneal (i.p.) injection or orogastric gavage (o.g.). At 7 days, protein and DNA content, crypt depth, and villus height were determined in the ileum. The premium dose and route was then given for 1 week either during (1 week after SBR) or after the adaptive phase (1 month after SBR). Differences between group means were analyzed using ANOVA. A P < 0.05 was considered significant.

RESULTS

EGF enhanced DNA and protein content, crypt depth, and villus height to the greatest extent at a dosage of 50 microg/kg/day by the o.g. route. EGF had no significant effect on enhancing adaptation when given after the adaptive response had already occurred.

CONCLUSIONS

Intestinal adaptation is optimally enhanced by a specific dose and route of EGF. Exogenous EGF enhances adaptation only during the adaptive response to SBR and not after it has already taken place. Determination of the best circumstances for EGF administration will permit a systematic approach toward understanding a mechanism for the beneficial effect of EGF during intestinal adaptation.

Authors+Show Affiliations

Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, 45229-3039, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9698525

Citation

Shin, C E., et al. "Epidermal Growth Factor Augments Adaptation Following Small Bowel Resection: Optimal Dosage, Route, and Timing of Administration." The Journal of Surgical Research, vol. 77, no. 1, 1998, pp. 11-6.
Shin CE, Helmrath MA, Falcone RA, et al. Epidermal growth factor augments adaptation following small bowel resection: optimal dosage, route, and timing of administration. J Surg Res. 1998;77(1):11-6.
Shin, C. E., Helmrath, M. A., Falcone, R. A., Fox, J. W., Duane, K. R., Erwin, C. R., & Warner, B. W. (1998). Epidermal growth factor augments adaptation following small bowel resection: optimal dosage, route, and timing of administration. The Journal of Surgical Research, 77(1), 11-6.
Shin CE, et al. Epidermal Growth Factor Augments Adaptation Following Small Bowel Resection: Optimal Dosage, Route, and Timing of Administration. J Surg Res. 1998;77(1):11-6. PubMed PMID: 9698525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epidermal growth factor augments adaptation following small bowel resection: optimal dosage, route, and timing of administration. AU - Shin,C E, AU - Helmrath,M A, AU - Falcone,R A,Jr AU - Fox,J W, AU - Duane,K R, AU - Erwin,C R, AU - Warner,B W, PY - 1998/8/12/pubmed PY - 2001/3/28/medline PY - 1998/8/12/entrez SP - 11 EP - 6 JF - The Journal of surgical research JO - J Surg Res VL - 77 IS - 1 N2 - BACKGROUND: In assorted animal models of small bowel resection (SBR), exogenous epidermal growth factor (EGF) has been shown to augment intestinal adaptation. This study was designed to elucidate the optimal dose, route, and timing of exogenous EGF to boost adaptation in our murine model of SBR. METHODS: Male ICR mice underwent either 50% proximal SBR or sham surgery (bowel transection with reanastomosis) and then randomized to receive either saline or human recombinant EGF (5, 50, 150, or 300 microg/kg/day) by twice daily intraperitoneal (i.p.) injection or orogastric gavage (o.g.). At 7 days, protein and DNA content, crypt depth, and villus height were determined in the ileum. The premium dose and route was then given for 1 week either during (1 week after SBR) or after the adaptive phase (1 month after SBR). Differences between group means were analyzed using ANOVA. A P < 0.05 was considered significant. RESULTS: EGF enhanced DNA and protein content, crypt depth, and villus height to the greatest extent at a dosage of 50 microg/kg/day by the o.g. route. EGF had no significant effect on enhancing adaptation when given after the adaptive response had already occurred. CONCLUSIONS: Intestinal adaptation is optimally enhanced by a specific dose and route of EGF. Exogenous EGF enhances adaptation only during the adaptive response to SBR and not after it has already taken place. Determination of the best circumstances for EGF administration will permit a systematic approach toward understanding a mechanism for the beneficial effect of EGF during intestinal adaptation. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/9698525/Epidermal_growth_factor_augments_adaptation_following_small_bowel_resection:_optimal_dosage_route_and_timing_of_administration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(98)95336-7 DB - PRIME DP - Unbound Medicine ER -