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Characterization of plasmid regions of foodborne Yersinia enterocolitica biogroup 1A strains hybridizing to the Yersinia enterocolitica virulence plasmid.
Syst Appl Microbiol 1998; 21(2):201-11SA

Abstract

The aim of our study was to find out if plasmids of foodborne Yersinia enterocolitica biogroup 1A strains harbour genes related to the virulence genes located on the virulence plasmid pYV of Yersinia enterocolitica. The foodborne strains were isolated from pork, as pigs are considered as an important reservoir for enteropathogenic Y. enterocolitica 0:3 and 0:9 strains. The plasmids of the foodborne strains were characterized by restriction enzyme analysis and hybridized to the virulence plasmid pYV of pathogenic Y. enterocolitica strains (0:3 biogroup 4; 0:9 biogroup 2). In several cases the plasmids of the foodborne strains showed homologies to parts of the pYV plasmid. Analysis of the hybridizing regions revealed that genes involved in replication, sequences of transposable elements and an endonuclease gene caused the observed hybridization to the virulence plasmid. In cause of the study also a remnant of a Tn3-like transposon was shown to be present adjacent to the yadA gene on the pYV plasmid. Although there is evidence that at least some strains of Y. enterocolitica biogroup 1A might possess pathogenic properties none of the well known plasmid encoded virulence genes were present on the plasmids of the investigated foodborne biogroup 1A strains.

Authors+Show Affiliations

Robert Koch-Institut, Fachbereich Genetik/Gentechnik, Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9704108

Citation

Hoffmann, B, et al. "Characterization of Plasmid Regions of Foodborne Yersinia Enterocolitica Biogroup 1A Strains Hybridizing to the Yersinia Enterocolitica Virulence Plasmid." Systematic and Applied Microbiology, vol. 21, no. 2, 1998, pp. 201-11.
Hoffmann B, Strauch E, Gewinner C, et al. Characterization of plasmid regions of foodborne Yersinia enterocolitica biogroup 1A strains hybridizing to the Yersinia enterocolitica virulence plasmid. Syst Appl Microbiol. 1998;21(2):201-11.
Hoffmann, B., Strauch, E., Gewinner, C., Nattermann, H., & Appel, B. (1998). Characterization of plasmid regions of foodborne Yersinia enterocolitica biogroup 1A strains hybridizing to the Yersinia enterocolitica virulence plasmid. Systematic and Applied Microbiology, 21(2), pp. 201-11.
Hoffmann B, et al. Characterization of Plasmid Regions of Foodborne Yersinia Enterocolitica Biogroup 1A Strains Hybridizing to the Yersinia Enterocolitica Virulence Plasmid. Syst Appl Microbiol. 1998;21(2):201-11. PubMed PMID: 9704108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of plasmid regions of foodborne Yersinia enterocolitica biogroup 1A strains hybridizing to the Yersinia enterocolitica virulence plasmid. AU - Hoffmann,B, AU - Strauch,E, AU - Gewinner,C, AU - Nattermann,H, AU - Appel,B, PY - 1998/8/15/pubmed PY - 1998/8/15/medline PY - 1998/8/15/entrez SP - 201 EP - 11 JF - Systematic and applied microbiology JO - Syst. Appl. Microbiol. VL - 21 IS - 2 N2 - The aim of our study was to find out if plasmids of foodborne Yersinia enterocolitica biogroup 1A strains harbour genes related to the virulence genes located on the virulence plasmid pYV of Yersinia enterocolitica. The foodborne strains were isolated from pork, as pigs are considered as an important reservoir for enteropathogenic Y. enterocolitica 0:3 and 0:9 strains. The plasmids of the foodborne strains were characterized by restriction enzyme analysis and hybridized to the virulence plasmid pYV of pathogenic Y. enterocolitica strains (0:3 biogroup 4; 0:9 biogroup 2). In several cases the plasmids of the foodborne strains showed homologies to parts of the pYV plasmid. Analysis of the hybridizing regions revealed that genes involved in replication, sequences of transposable elements and an endonuclease gene caused the observed hybridization to the virulence plasmid. In cause of the study also a remnant of a Tn3-like transposon was shown to be present adjacent to the yadA gene on the pYV plasmid. Although there is evidence that at least some strains of Y. enterocolitica biogroup 1A might possess pathogenic properties none of the well known plasmid encoded virulence genes were present on the plasmids of the investigated foodborne biogroup 1A strains. SN - 0723-2020 UR - https://www.unboundmedicine.com/medline/citation/9704108/Characterization_of_plasmid_regions_of_foodborne_Yersinia_enterocolitica_biogroup_1A_strains_hybridizing_to_the_Yersinia_enterocolitica_virulence_plasmid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0723-2020(98)80024-6 DB - PRIME DP - Unbound Medicine ER -