Tags

Type your tag names separated by a space and hit enter

Association of different tumor necrosis factor alpha promoter allele frequencies with ankylosing spondylitis in HLA-B27 positive individuals.
Arthritis Rheum. 1998 Aug; 41(8):1489-92.AR

Abstract

OBJECTIVE

To investigate the potential association of tumor necrosis factor alpha (TNFalpha) promoter alleles with ankylosing spondylitis.

METHODS

DNA from 141 HLA-B27 positive Caucasian patients with ankylosing spondylitis and 46 B27-positive and 99 B27-negative healthy Caucasian controls was investigated by polymerase chain reaction amplification of the TNFalpha promoter region and subsequent dot-blot analysis with allele-specific oligonucleotides.

RESULTS

There was a significant decrease in the promoter alleles TNF-238.2 and TNF-308.2 in the ankylosing spondylitis group (266 wild-type alleles, 16 variant alleles) compared with the B27-positive (75 wildtype promoter alleles, 17 variant alleles; P<0.0004) and the B27-negative (159 wild-type promoter alleles, 39 variant alleles; P< 0.00001) control groups. Positivity for the variant promoter alleles conferred protection and a relative risk of 0.3 to B27-positive individuals.

CONCLUSION

These data indicate that allelic variations in the TNFalpha promoter influence disease susceptibility in HLA-B27 positive individuals. This protective effect of variant promoter alleles could be related to differences in TNFalpha production or could reflect the association of different B27 haplotypes with ankylosing spondylitis.

Authors+Show Affiliations

Johannes Gutenberg Universität Mainz, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9704649

Citation

Höhler, T, et al. "Association of Different Tumor Necrosis Factor Alpha Promoter Allele Frequencies With Ankylosing Spondylitis in HLA-B27 Positive Individuals." Arthritis and Rheumatism, vol. 41, no. 8, 1998, pp. 1489-92.
Höhler T, Schäper T, Schneider PM, et al. Association of different tumor necrosis factor alpha promoter allele frequencies with ankylosing spondylitis in HLA-B27 positive individuals. Arthritis Rheum. 1998;41(8):1489-92.
Höhler, T., Schäper, T., Schneider, P. M., Meyer zum Büschenfelde, K. H., & Märker-Hermann, E. (1998). Association of different tumor necrosis factor alpha promoter allele frequencies with ankylosing spondylitis in HLA-B27 positive individuals. Arthritis and Rheumatism, 41(8), 1489-92.
Höhler T, et al. Association of Different Tumor Necrosis Factor Alpha Promoter Allele Frequencies With Ankylosing Spondylitis in HLA-B27 Positive Individuals. Arthritis Rheum. 1998;41(8):1489-92. PubMed PMID: 9704649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of different tumor necrosis factor alpha promoter allele frequencies with ankylosing spondylitis in HLA-B27 positive individuals. AU - Höhler,T, AU - Schäper,T, AU - Schneider,P M, AU - Meyer zum Büschenfelde,K H, AU - Märker-Hermann,E, PY - 1998/8/15/pubmed PY - 1998/8/15/medline PY - 1998/8/15/entrez SP - 1489 EP - 92 JF - Arthritis and rheumatism JO - Arthritis Rheum VL - 41 IS - 8 N2 - OBJECTIVE: To investigate the potential association of tumor necrosis factor alpha (TNFalpha) promoter alleles with ankylosing spondylitis. METHODS: DNA from 141 HLA-B27 positive Caucasian patients with ankylosing spondylitis and 46 B27-positive and 99 B27-negative healthy Caucasian controls was investigated by polymerase chain reaction amplification of the TNFalpha promoter region and subsequent dot-blot analysis with allele-specific oligonucleotides. RESULTS: There was a significant decrease in the promoter alleles TNF-238.2 and TNF-308.2 in the ankylosing spondylitis group (266 wild-type alleles, 16 variant alleles) compared with the B27-positive (75 wildtype promoter alleles, 17 variant alleles; P<0.0004) and the B27-negative (159 wild-type promoter alleles, 39 variant alleles; P< 0.00001) control groups. Positivity for the variant promoter alleles conferred protection and a relative risk of 0.3 to B27-positive individuals. CONCLUSION: These data indicate that allelic variations in the TNFalpha promoter influence disease susceptibility in HLA-B27 positive individuals. This protective effect of variant promoter alleles could be related to differences in TNFalpha production or could reflect the association of different B27 haplotypes with ankylosing spondylitis. SN - 0004-3591 UR - https://www.unboundmedicine.com/medline/citation/9704649/Association_of_different_tumor_necrosis_factor_alpha_promoter_allele_frequencies_with_ankylosing_spondylitis_in_HLA_B27_positive_individuals_ L2 - https://doi.org/10.1002/1529-0131(199808)41:8&lt;1489::AID-ART20&gt;3.0.CO;2-5 DB - PRIME DP - Unbound Medicine ER -