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Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion.
J Immunol. 1998 Aug 15; 161(4):1589-93.JI

Abstract

NK cells induce MHC class II molecules on the surface of allogeneic endothelial cells in an adhesion-dependent, IFN-gamma-independent manner. Here, we demonstrate that NK cells induce HLA-DR on the surface of a mutant cell line that is defective in IFN-gamma-induced MHC class II expression. RNA analysis in these cells and in a cell line that is defective in class II transactivator (CIITA) demonstrates that NK cell-induced HLA-DR alpha mRNA expression is also CIITA-independent. The Janus kinase-1-deficient cell line U4A expresses HLA-DR alpha mRNA in response to NK cell activation, and HLA-DR alpha promoter constructs transfected into these cells are induced by NK cells but not IFN-gamma. These data indicate that the IFN-gamma-independent component of the target cell HLA-DR expression induced by lymphocyte adhesion uses a signaling pathway that is distinct from the IFN-gamma-dependent mechanism and also suggest that CIITA is not required.

Authors+Show Affiliations

Division of Cardiovascular Medicine, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536, USA. mark.collinge@yale.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9712019

Citation

Collinge, M, et al. "Class II Transactivator-independent Endothelial Cell MHC Class II Gene Activation Induced By Lymphocyte Adhesion." Journal of Immunology (Baltimore, Md. : 1950), vol. 161, no. 4, 1998, pp. 1589-93.
Collinge M, Pardi R, Bender JR. Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion. J Immunol. 1998;161(4):1589-93.
Collinge, M., Pardi, R., & Bender, J. R. (1998). Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion. Journal of Immunology (Baltimore, Md. : 1950), 161(4), 1589-93.
Collinge M, Pardi R, Bender JR. Class II Transactivator-independent Endothelial Cell MHC Class II Gene Activation Induced By Lymphocyte Adhesion. J Immunol. 1998 Aug 15;161(4):1589-93. PubMed PMID: 9712019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion. AU - Collinge,M, AU - Pardi,R, AU - Bender,J R, PY - 1998/8/26/pubmed PY - 1998/8/26/medline PY - 1998/8/26/entrez SP - 1589 EP - 93 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 161 IS - 4 N2 - NK cells induce MHC class II molecules on the surface of allogeneic endothelial cells in an adhesion-dependent, IFN-gamma-independent manner. Here, we demonstrate that NK cells induce HLA-DR on the surface of a mutant cell line that is defective in IFN-gamma-induced MHC class II expression. RNA analysis in these cells and in a cell line that is defective in class II transactivator (CIITA) demonstrates that NK cell-induced HLA-DR alpha mRNA expression is also CIITA-independent. The Janus kinase-1-deficient cell line U4A expresses HLA-DR alpha mRNA in response to NK cell activation, and HLA-DR alpha promoter constructs transfected into these cells are induced by NK cells but not IFN-gamma. These data indicate that the IFN-gamma-independent component of the target cell HLA-DR expression induced by lymphocyte adhesion uses a signaling pathway that is distinct from the IFN-gamma-dependent mechanism and also suggest that CIITA is not required. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9712019/Class_II_transactivator_independent_endothelial_cell_MHC_class_II_gene_activation_induced_by_lymphocyte_adhesion_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9712019 DB - PRIME DP - Unbound Medicine ER -