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Inactivation of aconitase and oxoglutarate dehydrogenase in skeletal muscle in vitro by superoxide anions and/or nitric oxide.
Biochem Biophys Res Commun. 1998 Aug 19; 249(2):512-6.BB

Abstract

Strips of rat soleus muscle were incubated in media containing a superoxide generating system and/or the nitric oxide donor sodium nitroprusside (SNP) before the maximal catalytic activities of aconitase, citrate synthase, and oxoglutarate dehydrogenase were measured. The maximal activities of aconitase and oxoglutarate dehydrogenase were both decreased by 25-30% by superoxide anions; however, only the maximal activity of aconitase was decreased, by approximately 50%, by incubation of muscles with SNP. Furthermore, when both superoxide and NO were present in the medium, aconitase activity was decreased by 70%. The maximal activity of citrate synthase was not affected by any of the treatments. This is the first time that superoxide anions or NO has been shown to inactivate aconitase and oxoglutarate dehydrogenase in skeletal muscle. It is suggested that these effects may be responsible for some alterations in skeletal muscle metabolism, and these possibilities are discussed.

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Authors+Show Affiliations

Andersson U
Department of Biochemistry, University of Oxford, United Kingdom.
Leighton B
No affiliation info available
Young ME
No affiliation info available
Blomstrand E
No affiliation info available
Newsholme EA
No affiliation info available

MeSH

Aconitate HydrataseAnimalsAnionsCitrate (si)-SynthaseEnzyme InhibitorsKetoglutarate Dehydrogenase ComplexMaleMuscle, SkeletalNitric OxideNitroprussideRatsRats, WistarSuperoxides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9712727

Citation

Andersson, U, et al. "Inactivation of Aconitase and Oxoglutarate Dehydrogenase in Skeletal Muscle in Vitro By Superoxide Anions And/or Nitric Oxide." Biochemical and Biophysical Research Communications, vol. 249, no. 2, 1998, pp. 512-6.
Andersson U, Leighton B, Young ME, et al. Inactivation of aconitase and oxoglutarate dehydrogenase in skeletal muscle in vitro by superoxide anions and/or nitric oxide. Biochem Biophys Res Commun. 1998;249(2):512-6.
Andersson, U., Leighton, B., Young, M. E., Blomstrand, E., & Newsholme, E. A. (1998). Inactivation of aconitase and oxoglutarate dehydrogenase in skeletal muscle in vitro by superoxide anions and/or nitric oxide. Biochemical and Biophysical Research Communications, 249(2), 512-6.
Andersson U, et al. Inactivation of Aconitase and Oxoglutarate Dehydrogenase in Skeletal Muscle in Vitro By Superoxide Anions And/or Nitric Oxide. Biochem Biophys Res Commun. 1998 Aug 19;249(2):512-6. PubMed PMID: 9712727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivation of aconitase and oxoglutarate dehydrogenase in skeletal muscle in vitro by superoxide anions and/or nitric oxide. AU - Andersson,U, AU - Leighton,B, AU - Young,M E, AU - Blomstrand,E, AU - Newsholme,E A, PY - 1998/8/26/pubmed PY - 1998/8/26/medline PY - 1998/8/26/entrez SP - 512 EP - 6 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 249 IS - 2 N2 - Strips of rat soleus muscle were incubated in media containing a superoxide generating system and/or the nitric oxide donor sodium nitroprusside (SNP) before the maximal catalytic activities of aconitase, citrate synthase, and oxoglutarate dehydrogenase were measured. The maximal activities of aconitase and oxoglutarate dehydrogenase were both decreased by 25-30% by superoxide anions; however, only the maximal activity of aconitase was decreased, by approximately 50%, by incubation of muscles with SNP. Furthermore, when both superoxide and NO were present in the medium, aconitase activity was decreased by 70%. The maximal activity of citrate synthase was not affected by any of the treatments. This is the first time that superoxide anions or NO has been shown to inactivate aconitase and oxoglutarate dehydrogenase in skeletal muscle. It is suggested that these effects may be responsible for some alterations in skeletal muscle metabolism, and these possibilities are discussed. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/9712727/Inactivation_of_aconitase_and_oxoglutarate_dehydrogenase_in_skeletal_muscle_in_vitro_by_superoxide_anions_and/or_nitric_oxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(98)99171-9 DB - PRIME DP - Unbound Medicine ER -