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Identification of cocaine analytes in fingernail and toenail specimens.
J Forensic Sci. 1998 Sep; 43(5):974-9.JF

Abstract

Fingernail and toenail specimens were obtained from 18 suspected cocaine users. The nails were cut, heated under methanolic reflux, and the methanolic extracts were purified by solid-phase extraction. Gas chromatography/mass spectrometry was utilized for the qualitative and quantitative analysis of nine cocaine analytes. Comparison of conventional postmortem analysis of blood and urine with nail analysis revealed a marked increase in the detection of cocaine use by nail analysis. Cocaine analytes were present in 14 (82.3%) subjects utilizing nail analysis. Out of those 14 subjects, only 5 (27.7%) were positive by conventional postmortem drug analysis. Cocaine and benzoylecgonine were the predominant analytes in all positive nail specimens. Anhydroecgonine methyl ester, ecgonine methyl ester, ecgonine ethyl ester, cocaethylene, norcocaine, and norbenzoylecgonine were detected in a limited number of specimens. The ratio of cocaine to benzoylecgonine ranged from 2-10:1. These findings suggest that nails may be a useful alternative matrix for the detection of cocaine exposure.

Authors+Show Affiliations

Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville 32610, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9729814

Citation

Garside, D, et al. "Identification of Cocaine Analytes in Fingernail and Toenail Specimens." Journal of Forensic Sciences, vol. 43, no. 5, 1998, pp. 974-9.
Garside D, Ropero-Miller JD, Goldberger BA, et al. Identification of cocaine analytes in fingernail and toenail specimens. J Forensic Sci. 1998;43(5):974-9.
Garside, D., Ropero-Miller, J. D., Goldberger, B. A., Hamilton, W. F., & Maples, W. R. (1998). Identification of cocaine analytes in fingernail and toenail specimens. Journal of Forensic Sciences, 43(5), 974-9.
Garside D, et al. Identification of Cocaine Analytes in Fingernail and Toenail Specimens. J Forensic Sci. 1998;43(5):974-9. PubMed PMID: 9729814.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of cocaine analytes in fingernail and toenail specimens. AU - Garside,D, AU - Ropero-Miller,J D, AU - Goldberger,B A, AU - Hamilton,W F, AU - Maples,W R, PY - 1998/9/8/pubmed PY - 1998/9/8/medline PY - 1998/9/8/entrez SP - 974 EP - 9 JF - Journal of forensic sciences JO - J Forensic Sci VL - 43 IS - 5 N2 - Fingernail and toenail specimens were obtained from 18 suspected cocaine users. The nails were cut, heated under methanolic reflux, and the methanolic extracts were purified by solid-phase extraction. Gas chromatography/mass spectrometry was utilized for the qualitative and quantitative analysis of nine cocaine analytes. Comparison of conventional postmortem analysis of blood and urine with nail analysis revealed a marked increase in the detection of cocaine use by nail analysis. Cocaine analytes were present in 14 (82.3%) subjects utilizing nail analysis. Out of those 14 subjects, only 5 (27.7%) were positive by conventional postmortem drug analysis. Cocaine and benzoylecgonine were the predominant analytes in all positive nail specimens. Anhydroecgonine methyl ester, ecgonine methyl ester, ecgonine ethyl ester, cocaethylene, norcocaine, and norbenzoylecgonine were detected in a limited number of specimens. The ratio of cocaine to benzoylecgonine ranged from 2-10:1. These findings suggest that nails may be a useful alternative matrix for the detection of cocaine exposure. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/9729814/Identification_of_cocaine_analytes_in_fingernail_and_toenail_specimens_ DB - PRIME DP - Unbound Medicine ER -