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Comparison of cytochemical staining, immunofluorescence and PCR for diagnosis of pneumocystis carinii on sputum samples.
Scand J Infect Dis. 1998; 30(2):125-8.SJ

Abstract

Detection of P. carinii has increased with the use of polymerase chain reaction (PCR), particularly in sputum samples. In this study, sputum samples obtained from 30 immunosuppressed patients with respiratory symptoms (12 HIV-infected) were tested by standard cytochemical staining (Giemsa and methenamine silver), immunofluorescence (IF) staining and PCR for detection of P. carinii and the results were compared. Pneumocystis carinii was detected in 4, 8 and 13 sputum samples by cytological staining, IF test and PCR, respectively. Specific amplification bands were obtained in all sputum samples that were positive by both other tests. All tests gave negative results in sputum samples obtained from 5 HIV-infected asymptomatic patients and 22 non-immunosuppressed tuberculosis patients. Our observations suggest that PCR results were well correlated with P. carinii pneumonia (PCP), especially in non-HIV-infected patients. However, PCR positivity obtained in HIV-infected patients could be misleading in the diagnosis of PCP without careful clinical evaluation. Positive results obtained by Giemsa staining or IF test confirm diagnosis of PCP authoritatively. As a result, we suggest testing sputum samples by both PCR and IF techniques for detection of P. carinii.

Authors+Show Affiliations

Department of Medicine, Hacettepe University, School of Medicine, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article

Language

eng

PubMed ID

9730296

Citation

Tuncer, S, et al. "Comparison of Cytochemical Staining, Immunofluorescence and PCR for Diagnosis of Pneumocystis Carinii On Sputum Samples." Scandinavian Journal of Infectious Diseases, vol. 30, no. 2, 1998, pp. 125-8.
Tuncer S, Ergüven S, Kocagöz S, et al. Comparison of cytochemical staining, immunofluorescence and PCR for diagnosis of pneumocystis carinii on sputum samples. Scand J Infect Dis. 1998;30(2):125-8.
Tuncer, S., Ergüven, S., Kocagöz, S., & Unal, S. (1998). Comparison of cytochemical staining, immunofluorescence and PCR for diagnosis of pneumocystis carinii on sputum samples. Scandinavian Journal of Infectious Diseases, 30(2), 125-8.
Tuncer S, et al. Comparison of Cytochemical Staining, Immunofluorescence and PCR for Diagnosis of Pneumocystis Carinii On Sputum Samples. Scand J Infect Dis. 1998;30(2):125-8. PubMed PMID: 9730296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of cytochemical staining, immunofluorescence and PCR for diagnosis of pneumocystis carinii on sputum samples. AU - Tuncer,S, AU - Ergüven,S, AU - Kocagöz,S, AU - Unal,S, PY - 1998/9/8/pubmed PY - 1998/9/8/medline PY - 1998/9/8/entrez SP - 125 EP - 8 JF - Scandinavian journal of infectious diseases JO - Scand J Infect Dis VL - 30 IS - 2 N2 - Detection of P. carinii has increased with the use of polymerase chain reaction (PCR), particularly in sputum samples. In this study, sputum samples obtained from 30 immunosuppressed patients with respiratory symptoms (12 HIV-infected) were tested by standard cytochemical staining (Giemsa and methenamine silver), immunofluorescence (IF) staining and PCR for detection of P. carinii and the results were compared. Pneumocystis carinii was detected in 4, 8 and 13 sputum samples by cytological staining, IF test and PCR, respectively. Specific amplification bands were obtained in all sputum samples that were positive by both other tests. All tests gave negative results in sputum samples obtained from 5 HIV-infected asymptomatic patients and 22 non-immunosuppressed tuberculosis patients. Our observations suggest that PCR results were well correlated with P. carinii pneumonia (PCP), especially in non-HIV-infected patients. However, PCR positivity obtained in HIV-infected patients could be misleading in the diagnosis of PCP without careful clinical evaluation. Positive results obtained by Giemsa staining or IF test confirm diagnosis of PCP authoritatively. As a result, we suggest testing sputum samples by both PCR and IF techniques for detection of P. carinii. SN - 0036-5548 UR - https://www.unboundmedicine.com/medline/citation/9730296/Comparison_of_cytochemical_staining_immunofluorescence_and_PCR_for_diagnosis_of_pneumocystis_carinii_on_sputum_samples_ L2 - https://www.tandfonline.com/doi/full/10.1080/003655498750003483 DB - PRIME DP - Unbound Medicine ER -