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Isotope dilution-liquid chromatography/electrospray ionization-tandem mass spectrometry for the determination of serum thyroxine as a potential reference method.
Rapid Commun Mass Spectrom. 1998; 12(16):1099-103.RC

Abstract

A new candidate reference method is presented for the determination of thyroxine in serum. The method is based on isotope dilution-liquid chromatography/tandem mass spectrometry using electrospray for ionization. The internal standard used was 13C6-thyroxine, sample pretreatment consisted of protein precipitation and a two-step liquid/liquid extraction procedure, HPLC was performed on a C-18 column with an eluent containing methanol/water/formic acid (60:40:0.1, by volume), and finally thyroxine and its isotopically labeled analogue were measured in the selected reaction monitoring mode for the transitions m/z 777.7--> 731.7 and m/z 783.7--> 737.7, respectively. The detection limit for thyroxine was 6 pg, the within-run coefficient of variation was 1.1%. The samples were measured in six-fold: in duplicate on three independent days. The mean overall coefficient of variation of the method was 1.6%. The new method was evaluated by measuring nine control sera previously determined by an existing ID-GC/MS method. The differences between the results of the two methods ranged from-1.6% to +3.3%, with a mean of +0.2%.

Authors+Show Affiliations

De Brabandere VI
Laboratoria voor Medische Biochemie en Klinische Analyse, Faculty of Pharmaceutical Sciences, University of Ghent, Belgium.
Hou P
No affiliation info available
Stöckl D
No affiliation info available
Thienpont LM
No affiliation info available
De Leenheer AP
No affiliation info available

MeSH

CalibrationChromatography, High Pressure LiquidGas Chromatography-Mass SpectrometryHumansMass SpectrometryReference ValuesReproducibility of ResultsSolutionsThyroxine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9737016

Citation

De Brabandere, V I., et al. "Isotope Dilution-liquid Chromatography/electrospray Ionization-tandem Mass Spectrometry for the Determination of Serum Thyroxine as a Potential Reference Method." Rapid Communications in Mass Spectrometry : RCM, vol. 12, no. 16, 1998, pp. 1099-103.
De Brabandere VI, Hou P, Stöckl D, et al. Isotope dilution-liquid chromatography/electrospray ionization-tandem mass spectrometry for the determination of serum thyroxine as a potential reference method. Rapid Commun Mass Spectrom. 1998;12(16):1099-103.
De Brabandere, V. I., Hou, P., Stöckl, D., Thienpont, L. M., & De Leenheer, A. P. (1998). Isotope dilution-liquid chromatography/electrospray ionization-tandem mass spectrometry for the determination of serum thyroxine as a potential reference method. Rapid Communications in Mass Spectrometry : RCM, 12(16), 1099-103.
De Brabandere VI, et al. Isotope Dilution-liquid Chromatography/electrospray Ionization-tandem Mass Spectrometry for the Determination of Serum Thyroxine as a Potential Reference Method. Rapid Commun Mass Spectrom. 1998;12(16):1099-103. PubMed PMID: 9737016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isotope dilution-liquid chromatography/electrospray ionization-tandem mass spectrometry for the determination of serum thyroxine as a potential reference method. AU - De Brabandere,V I, AU - Hou,P, AU - Stöckl,D, AU - Thienpont,L M, AU - De Leenheer,A P, PY - 1998/9/16/pubmed PY - 2000/6/20/medline PY - 1998/9/16/entrez SP - 1099 EP - 103 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 12 IS - 16 N2 - A new candidate reference method is presented for the determination of thyroxine in serum. The method is based on isotope dilution-liquid chromatography/tandem mass spectrometry using electrospray for ionization. The internal standard used was 13C6-thyroxine, sample pretreatment consisted of protein precipitation and a two-step liquid/liquid extraction procedure, HPLC was performed on a C-18 column with an eluent containing methanol/water/formic acid (60:40:0.1, by volume), and finally thyroxine and its isotopically labeled analogue were measured in the selected reaction monitoring mode for the transitions m/z 777.7--> 731.7 and m/z 783.7--> 737.7, respectively. The detection limit for thyroxine was 6 pg, the within-run coefficient of variation was 1.1%. The samples were measured in six-fold: in duplicate on three independent days. The mean overall coefficient of variation of the method was 1.6%. The new method was evaluated by measuring nine control sera previously determined by an existing ID-GC/MS method. The differences between the results of the two methods ranged from-1.6% to +3.3%, with a mean of +0.2%. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/9737016/Isotope_dilution_liquid_chromatography/electrospray_ionization_tandem_mass_spectrometry_for_the_determination_of_serum_thyroxine_as_a_potential_reference_method_ DB - PRIME DP - Unbound Medicine ER -
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