Tags

Type your tag names separated by a space and hit enter

Simple analysis of tetracyclic antidepressants in blood using headspace-solid-phase microextraction and GC-MS.
J Anal Toxicol. 1998 Sep; 22(5):396-400.JA

Abstract

A simple and sensitive method for analysis of three tetracyclic antidepressants, maprotiline, mianserin, and setiptiline, in human whole blood was developed using headspace-solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS). A vial containing a blood sample, sodium hydroxide, and imipramine as an internal standard was heated at 120 degrees C. The extraction fiber of the SPME was exposed for 45 min in the headspace of the vial. The compounds absorbed on the fiber were desorbed by exposing the fiber in the injection port of a GC-MS. The calibration curves, using an internal standard method, demonstrated good linearity throughout the concentration range from 0.005 to 5.0 microg/g for mianserin and setiptiline and from 0.025 to 25 microg/g for maprotiline. No interferences were found, and the time for analysis was 60 min for one sample. In addition, this proposed method was applied to a medicolegal case in which the cause of death was suspected to be acute setiptiline poisoning. Setiptiline was detected in the left and right heart blood samples of the victim at concentrations of 1.77 and 0.78 microg/g, respectively.

Authors+Show Affiliations

Department of Legal Medicine, Hiroshima University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9737336

Citation

Namera, A, et al. "Simple Analysis of Tetracyclic Antidepressants in Blood Using Headspace-solid-phase Microextraction and GC-MS." Journal of Analytical Toxicology, vol. 22, no. 5, 1998, pp. 396-400.
Namera A, Watanabe T, Yashiki M, et al. Simple analysis of tetracyclic antidepressants in blood using headspace-solid-phase microextraction and GC-MS. J Anal Toxicol. 1998;22(5):396-400.
Namera, A., Watanabe, T., Yashiki, M., Iwasaki, Y., & Kojima, T. (1998). Simple analysis of tetracyclic antidepressants in blood using headspace-solid-phase microextraction and GC-MS. Journal of Analytical Toxicology, 22(5), 396-400.
Namera A, et al. Simple Analysis of Tetracyclic Antidepressants in Blood Using Headspace-solid-phase Microextraction and GC-MS. J Anal Toxicol. 1998;22(5):396-400. PubMed PMID: 9737336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple analysis of tetracyclic antidepressants in blood using headspace-solid-phase microextraction and GC-MS. AU - Namera,A, AU - Watanabe,T, AU - Yashiki,M, AU - Iwasaki,Y, AU - Kojima,T, PY - 1998/9/16/pubmed PY - 1998/9/16/medline PY - 1998/9/16/entrez SP - 396 EP - 400 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 22 IS - 5 N2 - A simple and sensitive method for analysis of three tetracyclic antidepressants, maprotiline, mianserin, and setiptiline, in human whole blood was developed using headspace-solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS). A vial containing a blood sample, sodium hydroxide, and imipramine as an internal standard was heated at 120 degrees C. The extraction fiber of the SPME was exposed for 45 min in the headspace of the vial. The compounds absorbed on the fiber were desorbed by exposing the fiber in the injection port of a GC-MS. The calibration curves, using an internal standard method, demonstrated good linearity throughout the concentration range from 0.005 to 5.0 microg/g for mianserin and setiptiline and from 0.025 to 25 microg/g for maprotiline. No interferences were found, and the time for analysis was 60 min for one sample. In addition, this proposed method was applied to a medicolegal case in which the cause of death was suspected to be acute setiptiline poisoning. Setiptiline was detected in the left and right heart blood samples of the victim at concentrations of 1.77 and 0.78 microg/g, respectively. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/9737336/Simple_analysis_of_tetracyclic_antidepressants_in_blood_using_headspace_solid_phase_microextraction_and_GC_MS_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/22.5.396 DB - PRIME DP - Unbound Medicine ER -