Tags

Type your tag names separated by a space and hit enter

The proregion of papaya proteinase IV inhibits Colorado potato beetle digestive cysteine proteinases.
FEBS Lett. 1998 Sep 04; 434(3):401-5.FL

Abstract

Three distinct digestive protease systems were induced in larvae of the herbivorous pest, Colorado potato beetle (CPB; Leptinotarsa decemlineata Say), and used as a model to assess the ability of the proregion of papaya proteinase IV (PPIV; glycyl endopeptidase, EC 3.4.22.25) to act as an inhibitor of insect digestive cysteine proteinases. As shown by gelatin/PAGE and complementary inhibition assays, a recombinant form of the proregion produced in Escherichia coli inhibited a fraction of the insect proteases also inhibited by the well-characterized inhibitor of cysteine proteinases, oryzacystatin I (OCI). In contrast with OCI, the inhibitory potency of the proregion was affected by an increase of the temperature, suggesting a certain alteration of its structural integrity by the insect non-target proteases. This apparent susceptibility to proteolysis was confirmed by SDS-PAGE, after challenging the proregion with the different insect extracts. As seen on gel, selective inhibition of the insect aspartate proteinase, cathepsin D, with the inhibitor pepstatin A preserved the activity of the proregion against cysteine proteinases by preventing its hydrolysis. Taken together, these observations suggest the potential of plant protease proregions as regulators of cysteine proteinases in biotechnological systems, and show the ability of protease inhibitors to preserve the integrity of 'companion' defense-related proteins from the action of insensitive proteases in target pests.

Links

Publisher Full Text
linkinghub.elsevier.com
onlinelibrary.wiley.com

Authors+Show Affiliations

Visal S
Centre de Recherche en Horticulture, FSAA, Université Laval, Sainte-Foy, Qué., Canada.
Taylor MA
No affiliation info available
Michaud D
No affiliation info available

MeSH

AnimalsColeopteraCystatinsCysteine EndopeptidasesCysteine Proteinase InhibitorsDigestive SystemLarva

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9742962

Citation

Visal, S, et al. "The Proregion of Papaya Proteinase IV Inhibits Colorado Potato Beetle Digestive Cysteine Proteinases." FEBS Letters, vol. 434, no. 3, 1998, pp. 401-5.
Visal S, Taylor MA, Michaud D. The proregion of papaya proteinase IV inhibits Colorado potato beetle digestive cysteine proteinases. FEBS Lett. 1998;434(3):401-5.
Visal, S., Taylor, M. A., & Michaud, D. (1998). The proregion of papaya proteinase IV inhibits Colorado potato beetle digestive cysteine proteinases. FEBS Letters, 434(3), 401-5.
Visal S, Taylor MA, Michaud D. The Proregion of Papaya Proteinase IV Inhibits Colorado Potato Beetle Digestive Cysteine Proteinases. FEBS Lett. 1998 Sep 4;434(3):401-5. PubMed PMID: 9742962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The proregion of papaya proteinase IV inhibits Colorado potato beetle digestive cysteine proteinases. AU - Visal,S, AU - Taylor,M A, AU - Michaud,D, PY - 1998/9/22/pubmed PY - 1998/9/22/medline PY - 1998/9/22/entrez SP - 401 EP - 5 JF - FEBS letters JO - FEBS Lett VL - 434 IS - 3 N2 - Three distinct digestive protease systems were induced in larvae of the herbivorous pest, Colorado potato beetle (CPB; Leptinotarsa decemlineata Say), and used as a model to assess the ability of the proregion of papaya proteinase IV (PPIV; glycyl endopeptidase, EC 3.4.22.25) to act as an inhibitor of insect digestive cysteine proteinases. As shown by gelatin/PAGE and complementary inhibition assays, a recombinant form of the proregion produced in Escherichia coli inhibited a fraction of the insect proteases also inhibited by the well-characterized inhibitor of cysteine proteinases, oryzacystatin I (OCI). In contrast with OCI, the inhibitory potency of the proregion was affected by an increase of the temperature, suggesting a certain alteration of its structural integrity by the insect non-target proteases. This apparent susceptibility to proteolysis was confirmed by SDS-PAGE, after challenging the proregion with the different insect extracts. As seen on gel, selective inhibition of the insect aspartate proteinase, cathepsin D, with the inhibitor pepstatin A preserved the activity of the proregion against cysteine proteinases by preventing its hydrolysis. Taken together, these observations suggest the potential of plant protease proregions as regulators of cysteine proteinases in biotechnological systems, and show the ability of protease inhibitors to preserve the integrity of 'companion' defense-related proteins from the action of insensitive proteases in target pests. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/9742962/The_proregion_of_papaya_proteinase_IV_inhibits_Colorado_potato_beetle_digestive_cysteine_proteinases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(98)01018-7 DB - PRIME DP - Unbound Medicine ER -