Tags

Type your tag names separated by a space and hit enter

Role of peroxynitrite and poly (ADP-ribosyl) synthetase activation in cardiovascular derangement induced by zymosan in the rat.
Life Sci. 1998; 63(11):923-33.LS

Abstract

Peritoneal administration of zymosan in the rat induced a severe inflammatory process characterised by an increase in the plasma levels of nitrite and nitrate, stable metabolites of nitric oxide (NO) and in the levels of peroxynitrite, as measured by the oxidation of the fluorescent dye dihydrorhodamine 123, at 18 hours zymosan challenge. Immunohistochemical examination demonstrated a marked increase in the immunoreactivity to nitrotyrosine, a specific "footprint" of peroxynitrite, in the aorta of zymosan-shocked rats. In ex vivo experiments, thoracic aorta rings of zymosan-treated rats showed a reduced contraction to noradrenaline and reduced responsiveness to the relaxant effect to acetylcholine (vascular hyporeactivity and endothelial dysfunction, respectively). Treatment of zymosan-shocked rats with 3-aminobenzamide or Nicotinamide, inhibitors of poly ADP-ribosil synthetase (PARS) activity reduced the production of peroxynitrite and significantly prevented the cardiovascular dysfunction. Our data suggest that peroxynitrite and PARS activation play a role in the zymosan-induced cardiovascular derangements in the rat.

Authors+Show Affiliations

Institute of Pharmacology, School of Medicine, University of Messina, Italy.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9747893

Citation

Cuzzocrea, S, et al. "Role of Peroxynitrite and Poly (ADP-ribosyl) Synthetase Activation in Cardiovascular Derangement Induced By Zymosan in the Rat." Life Sciences, vol. 63, no. 11, 1998, pp. 923-33.
Cuzzocrea S, Zingarelli B, Caputi AP. Role of peroxynitrite and poly (ADP-ribosyl) synthetase activation in cardiovascular derangement induced by zymosan in the rat. Life Sci. 1998;63(11):923-33.
Cuzzocrea, S., Zingarelli, B., & Caputi, A. P. (1998). Role of peroxynitrite and poly (ADP-ribosyl) synthetase activation in cardiovascular derangement induced by zymosan in the rat. Life Sciences, 63(11), 923-33.
Cuzzocrea S, Zingarelli B, Caputi AP. Role of Peroxynitrite and Poly (ADP-ribosyl) Synthetase Activation in Cardiovascular Derangement Induced By Zymosan in the Rat. Life Sci. 1998;63(11):923-33. PubMed PMID: 9747893.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of peroxynitrite and poly (ADP-ribosyl) synthetase activation in cardiovascular derangement induced by zymosan in the rat. AU - Cuzzocrea,S, AU - Zingarelli,B, AU - Caputi,A P, PY - 1998/9/25/pubmed PY - 1998/9/25/medline PY - 1998/9/25/entrez SP - 923 EP - 33 JF - Life sciences JO - Life Sci VL - 63 IS - 11 N2 - Peritoneal administration of zymosan in the rat induced a severe inflammatory process characterised by an increase in the plasma levels of nitrite and nitrate, stable metabolites of nitric oxide (NO) and in the levels of peroxynitrite, as measured by the oxidation of the fluorescent dye dihydrorhodamine 123, at 18 hours zymosan challenge. Immunohistochemical examination demonstrated a marked increase in the immunoreactivity to nitrotyrosine, a specific "footprint" of peroxynitrite, in the aorta of zymosan-shocked rats. In ex vivo experiments, thoracic aorta rings of zymosan-treated rats showed a reduced contraction to noradrenaline and reduced responsiveness to the relaxant effect to acetylcholine (vascular hyporeactivity and endothelial dysfunction, respectively). Treatment of zymosan-shocked rats with 3-aminobenzamide or Nicotinamide, inhibitors of poly ADP-ribosil synthetase (PARS) activity reduced the production of peroxynitrite and significantly prevented the cardiovascular dysfunction. Our data suggest that peroxynitrite and PARS activation play a role in the zymosan-induced cardiovascular derangements in the rat. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/9747893/Role_of_peroxynitrite_and_poly__ADP_ribosyl__synthetase_activation_in_cardiovascular_derangement_induced_by_zymosan_in_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024320598003506 DB - PRIME DP - Unbound Medicine ER -