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Autoradiographic visualisation of axonal transport of adenosine A1 receptors along the rat vagus nerve and characterisation of adenosine A1 receptor binding in the dorsal vagal complex of hypertensive and normotensive rats.
Brain Res 1998; 802(1-2):61-8BR

Abstract

The present study had employed in vitro receptor autoradiography with [3H]DPCPX to visualise the presence of adenosine A1 receptors on the rat nodose ganglion, which contains the perikarya of vagal afferent neurons projecting the the nucleus tractus solitarius (NTS). In addition, unilateral vagal ligation resulted in an accumulation of [3H]DPCPX binding adjacent to the ligatures, indication that adenosine A1 receptors are subject to axoplasmic flow along the rat vagus nerve. Radioligand binding assays were utilised to characterise the properties of adenosine A1 receptors in the dorsal vagal complex (NTS, area postrema and dorsal motor nucleus of the vagus) of pup and adult normotensive (Wistar Kyoto, WKY) and hypertensive (spontaneously hypertensive, SHR) rats. Saturation binding indicated that the affinity (KD) of [3H]DPCPX, and the binding site density (Bmax) were not different between the adult WKY and SHR, although the pup SHR had a lower KD value than the pup WKY rat. Competition binding assays revealed complex differences between the two rat strains; however, with respect to hypertension, the affinity of the selective adenosine A1 agonist, cyclohexyladenosine (CHA), was markedly reduced in the membranes from SHR (Ki approximately 93 nM) compared to WKY (approximately 6 nM). Such an observation is consistent with the attenuated responses of SHRs to intra-NTS injections of adenosine.

Authors+Show Affiliations

Department of Pharmacology, Monash University, Clayton, Victoria, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9748505

Citation

Krstew, E, et al. "Autoradiographic Visualisation of Axonal Transport of Adenosine A1 Receptors Along the Rat Vagus Nerve and Characterisation of Adenosine A1 Receptor Binding in the Dorsal Vagal Complex of Hypertensive and Normotensive Rats." Brain Research, vol. 802, no. 1-2, 1998, pp. 61-8.
Krstew E, Jarrott B, Lawrence AJ. Autoradiographic visualisation of axonal transport of adenosine A1 receptors along the rat vagus nerve and characterisation of adenosine A1 receptor binding in the dorsal vagal complex of hypertensive and normotensive rats. Brain Res. 1998;802(1-2):61-8.
Krstew, E., Jarrott, B., & Lawrence, A. J. (1998). Autoradiographic visualisation of axonal transport of adenosine A1 receptors along the rat vagus nerve and characterisation of adenosine A1 receptor binding in the dorsal vagal complex of hypertensive and normotensive rats. Brain Research, 802(1-2), pp. 61-8.
Krstew E, Jarrott B, Lawrence AJ. Autoradiographic Visualisation of Axonal Transport of Adenosine A1 Receptors Along the Rat Vagus Nerve and Characterisation of Adenosine A1 Receptor Binding in the Dorsal Vagal Complex of Hypertensive and Normotensive Rats. Brain Res. 1998 Aug 17;802(1-2):61-8. PubMed PMID: 9748505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autoradiographic visualisation of axonal transport of adenosine A1 receptors along the rat vagus nerve and characterisation of adenosine A1 receptor binding in the dorsal vagal complex of hypertensive and normotensive rats. AU - Krstew,E, AU - Jarrott,B, AU - Lawrence,A J, PY - 1998/9/28/pubmed PY - 1998/9/28/medline PY - 1998/9/28/entrez SP - 61 EP - 8 JF - Brain research JO - Brain Res. VL - 802 IS - 1-2 N2 - The present study had employed in vitro receptor autoradiography with [3H]DPCPX to visualise the presence of adenosine A1 receptors on the rat nodose ganglion, which contains the perikarya of vagal afferent neurons projecting the the nucleus tractus solitarius (NTS). In addition, unilateral vagal ligation resulted in an accumulation of [3H]DPCPX binding adjacent to the ligatures, indication that adenosine A1 receptors are subject to axoplasmic flow along the rat vagus nerve. Radioligand binding assays were utilised to characterise the properties of adenosine A1 receptors in the dorsal vagal complex (NTS, area postrema and dorsal motor nucleus of the vagus) of pup and adult normotensive (Wistar Kyoto, WKY) and hypertensive (spontaneously hypertensive, SHR) rats. Saturation binding indicated that the affinity (KD) of [3H]DPCPX, and the binding site density (Bmax) were not different between the adult WKY and SHR, although the pup SHR had a lower KD value than the pup WKY rat. Competition binding assays revealed complex differences between the two rat strains; however, with respect to hypertension, the affinity of the selective adenosine A1 agonist, cyclohexyladenosine (CHA), was markedly reduced in the membranes from SHR (Ki approximately 93 nM) compared to WKY (approximately 6 nM). Such an observation is consistent with the attenuated responses of SHRs to intra-NTS injections of adenosine. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/9748505/Autoradiographic_visualisation_of_axonal_transport_of_adenosine_A1_receptors_along_the_rat_vagus_nerve_and_characterisation_of_adenosine_A1_receptor_binding_in_the_dorsal_vagal_complex_of_hypertensive_and_normotensive_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(98)00567-8 DB - PRIME DP - Unbound Medicine ER -