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Molecular characterization of the virulence gene virG of pTiKU 12.
Mol Cells. 1998 Aug 31; 8(4):393-400.MC

Abstract

The virulence (vir) genes of Agrobacterium tumefaciens KU12, a Korean strain, were not induced by acetosyringone and the strain showed weak tumor forming ability and broad plant host ranges. We identified complete nucleotide sequence of virG of pTiKU12, an octopine Ti plasmid of this strain. When it was compared with those of other Ti plasmids, pTiKU12 virG contained an open reading frame (ORF) of 726 nucleotides which showed much lower homology (about 77%) than those (above 98%) already known among octopine Ti plasmids and it started with GTG codon instead of TTG found in other Ti plasmids. Only two vir boxes and one promoter region were confirmed in 5'-untranslated region instead of three vir boxes and two promoters which were found in pTiA6 virG. Nevertheless, important amino acids for the functional activity of VirG were so conserved that the virG included in pUCDG could complement a virG mutant Agrobacterium tumefaciens Mx19 in beta-galactosidase activity assays and on plant tumor tests.

Authors+Show Affiliations

Department of Biology, Korea University, Seoul.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9749525

Citation

Lee, S H., and W S. Sim. "Molecular Characterization of the Virulence Gene virG of pTiKU 12." Molecules and Cells, vol. 8, no. 4, 1998, pp. 393-400.
Lee SH, Sim WS. Molecular characterization of the virulence gene virG of pTiKU 12. Mol Cells. 1998;8(4):393-400.
Lee, S. H., & Sim, W. S. (1998). Molecular characterization of the virulence gene virG of pTiKU 12. Molecules and Cells, 8(4), 393-400.
Lee SH, Sim WS. Molecular Characterization of the Virulence Gene virG of pTiKU 12. Mol Cells. 1998 Aug 31;8(4):393-400. PubMed PMID: 9749525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of the virulence gene virG of pTiKU 12. AU - Lee,S H, AU - Sim,W S, PY - 1998/9/28/pubmed PY - 1998/9/28/medline PY - 1998/9/28/entrez SP - 393 EP - 400 JF - Molecules and cells JO - Mol. Cells VL - 8 IS - 4 N2 - The virulence (vir) genes of Agrobacterium tumefaciens KU12, a Korean strain, were not induced by acetosyringone and the strain showed weak tumor forming ability and broad plant host ranges. We identified complete nucleotide sequence of virG of pTiKU12, an octopine Ti plasmid of this strain. When it was compared with those of other Ti plasmids, pTiKU12 virG contained an open reading frame (ORF) of 726 nucleotides which showed much lower homology (about 77%) than those (above 98%) already known among octopine Ti plasmids and it started with GTG codon instead of TTG found in other Ti plasmids. Only two vir boxes and one promoter region were confirmed in 5'-untranslated region instead of three vir boxes and two promoters which were found in pTiA6 virG. Nevertheless, important amino acids for the functional activity of VirG were so conserved that the virG included in pUCDG could complement a virG mutant Agrobacterium tumefaciens Mx19 in beta-galactosidase activity assays and on plant tumor tests. SN - 1016-8478 UR - https://www.unboundmedicine.com/medline/citation/9749525/Molecular_characterization_of_the_virulence_gene_virG_of_pTiKU_12_ L2 - http://www.molcells.org/journal/view.html?year=1998&volume=8&number=4&spage=393 DB - PRIME DP - Unbound Medicine ER -