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[Distribution of E- and St-specific RAPD fragments in few genomes of triticeae].
Yi Chuan Xue Bao. 1998 Apr; 25(2):131-41.YC

Abstract

Two E genome--(including Ee and Eb) and two St-genome-specific RAPD markers were successfully cloned. Sequencing data indicated that the four DNA fragments were reported for the first time. Chromosome fluorescent in situ hybridization (FISH) showed that OPD-12(444) (E-specific) was a highly tandem repeat. OPF-03(1296) (Eb-specific), OPB-08(525) (St-specific), and OPN-01(817) (St-specific) were highly dispersed repetitive sequences. The FISH and Southern hybridization of genomic DNA also revealed that all of the four highly repetitive sequences were shared among relatively closer genomes of triticeae. The difference was mainly in the fragment length and the copy number. Whether a genome can amplify a specific sequence via RAPD is mainly dependent on if there are primering sites at both ends of this sequence in the genome. We also discussed the potentiality of these highly repetitive DNA fragments in detection of alien chromatin in wheat and determination of genome constitution of polyploid triticeae species.

Authors+Show Affiliations

Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

9752009

Citation

Zhang, X Y., et al. "[Distribution of E- and St-specific RAPD Fragments in Few Genomes of Triticeae]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 25, no. 2, 1998, pp. 131-41.
Zhang XY, Dong YS, LI P, et al. [Distribution of E- and St-specific RAPD fragments in few genomes of triticeae]. Yi Chuan Xue Bao. 1998;25(2):131-41.
Zhang, X. Y., Dong, Y. S., LI, P., & Wang, R. R. (1998). [Distribution of E- and St-specific RAPD fragments in few genomes of triticeae]. Yi Chuan Xue Bao = Acta Genetica Sinica, 25(2), 131-41.
Zhang XY, et al. [Distribution of E- and St-specific RAPD Fragments in Few Genomes of Triticeae]. Yi Chuan Xue Bao. 1998;25(2):131-41. PubMed PMID: 9752009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Distribution of E- and St-specific RAPD fragments in few genomes of triticeae]. AU - Zhang,X Y, AU - Dong,Y S, AU - LI,P, AU - Wang,R R, PY - 1998/9/30/pubmed PY - 1998/9/30/medline PY - 1998/9/30/entrez SP - 131 EP - 41 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 25 IS - 2 N2 - Two E genome--(including Ee and Eb) and two St-genome-specific RAPD markers were successfully cloned. Sequencing data indicated that the four DNA fragments were reported for the first time. Chromosome fluorescent in situ hybridization (FISH) showed that OPD-12(444) (E-specific) was a highly tandem repeat. OPF-03(1296) (Eb-specific), OPB-08(525) (St-specific), and OPN-01(817) (St-specific) were highly dispersed repetitive sequences. The FISH and Southern hybridization of genomic DNA also revealed that all of the four highly repetitive sequences were shared among relatively closer genomes of triticeae. The difference was mainly in the fragment length and the copy number. Whether a genome can amplify a specific sequence via RAPD is mainly dependent on if there are primering sites at both ends of this sequence in the genome. We also discussed the potentiality of these highly repetitive DNA fragments in detection of alien chromatin in wheat and determination of genome constitution of polyploid triticeae species. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/9752009/[Distribution_of_E__and_St_specific_RAPD_fragments_in_few_genomes_of_triticeae]_ L2 - https://antibodies.cancer.gov/detail/CPTC-SPP1-1 DB - PRIME DP - Unbound Medicine ER -