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Effects of milk and milk products on rectal mucosal cell proliferation in humans.
Cancer Epidemiol Biomarkers Prev. 1998 Sep; 7(9):757-66.CE

Abstract

Intake of dairy products and major dairy constituents (e.g., calcium) has been proposed to reduce the risk of colorectal cancer, although epidemiological studies have yielded inconclusive results. We conducted a randomized cross-over trial to test the effects of high- and low-dairy consumption diets on rectal mucosal proliferation, a possible intermediary marker for large bowel cancer. From a gastroenterology clinic at an academic medical center, we recruited 40 patients, ages 25-79 years, who had either a history of a large bowel adenoma or a first-degree relative with large bowel cancer. Participants completed a baseline questionnaire covering demographic characteristics, health history, and habits and a food frequency questionnaire. They were randomized to a 12-week diet of either high dairy intake (six dairy servings/day) or low dairy intake (<0.5 serving of dairy products/day), with an intervening 12-week washout period in which they were asked to resume their usual diet before crossing over to the alternate study diet for the last 12-week period of the study. Adherence to the study diets was monitored by a daily dairy intake checklist and periodic, unscheduled 24-h dietary recalls. Biopsies of the rectal mucosa were obtained at the beginning and end of each intervention phase. Two assays of rectal mucosal cell proliferation were performed: immunohistochemical determination of proliferating cell nuclear antigen and whole crypt mitotic count. We found no statistically significant changes in either of these proliferation measures as a result of high or low dairy intake. There was no correlation between the labeling index for proliferating cell nuclear antigen and whole crypt mitotic count; however, measures of the location and intensity of cell proliferation within the rectal crypt were highly correlated between the two assays. Thus, our study indicates that greater consumption of dairy products over a 12-week period does not change rectal mucosal cell proliferation.

Authors+Show Affiliations

Department of Community and Family Medicine, and the Norris Cotton Cancer Center, Hanover, New Hampshire 03756, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9752983

Citation

Karagas, M R., et al. "Effects of Milk and Milk Products On Rectal Mucosal Cell Proliferation in Humans." Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored By the American Society of Preventive Oncology, vol. 7, no. 9, 1998, pp. 757-66.
Karagas MR, Tosteson TD, Greenberg ER, et al. Effects of milk and milk products on rectal mucosal cell proliferation in humans. Cancer Epidemiol Biomarkers Prev. 1998;7(9):757-66.
Karagas, M. R., Tosteson, T. D., Greenberg, E. R., Rothstein, R. I., Roebuck, B. D., Herrin, M., & Ahnen, D. (1998). Effects of milk and milk products on rectal mucosal cell proliferation in humans. Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored By the American Society of Preventive Oncology, 7(9), 757-66.
Karagas MR, et al. Effects of Milk and Milk Products On Rectal Mucosal Cell Proliferation in Humans. Cancer Epidemiol Biomarkers Prev. 1998;7(9):757-66. PubMed PMID: 9752983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of milk and milk products on rectal mucosal cell proliferation in humans. AU - Karagas,M R, AU - Tosteson,T D, AU - Greenberg,E R, AU - Rothstein,R I, AU - Roebuck,B D, AU - Herrin,M, AU - Ahnen,D, PY - 1998/9/30/pubmed PY - 1998/9/30/medline PY - 1998/9/30/entrez SP - 757 EP - 66 JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology JO - Cancer Epidemiol Biomarkers Prev VL - 7 IS - 9 N2 - Intake of dairy products and major dairy constituents (e.g., calcium) has been proposed to reduce the risk of colorectal cancer, although epidemiological studies have yielded inconclusive results. We conducted a randomized cross-over trial to test the effects of high- and low-dairy consumption diets on rectal mucosal proliferation, a possible intermediary marker for large bowel cancer. From a gastroenterology clinic at an academic medical center, we recruited 40 patients, ages 25-79 years, who had either a history of a large bowel adenoma or a first-degree relative with large bowel cancer. Participants completed a baseline questionnaire covering demographic characteristics, health history, and habits and a food frequency questionnaire. They were randomized to a 12-week diet of either high dairy intake (six dairy servings/day) or low dairy intake (<0.5 serving of dairy products/day), with an intervening 12-week washout period in which they were asked to resume their usual diet before crossing over to the alternate study diet for the last 12-week period of the study. Adherence to the study diets was monitored by a daily dairy intake checklist and periodic, unscheduled 24-h dietary recalls. Biopsies of the rectal mucosa were obtained at the beginning and end of each intervention phase. Two assays of rectal mucosal cell proliferation were performed: immunohistochemical determination of proliferating cell nuclear antigen and whole crypt mitotic count. We found no statistically significant changes in either of these proliferation measures as a result of high or low dairy intake. There was no correlation between the labeling index for proliferating cell nuclear antigen and whole crypt mitotic count; however, measures of the location and intensity of cell proliferation within the rectal crypt were highly correlated between the two assays. Thus, our study indicates that greater consumption of dairy products over a 12-week period does not change rectal mucosal cell proliferation. SN - 1055-9965 UR - https://www.unboundmedicine.com/medline/citation/9752983/Effects_of_milk_and_milk_products_on_rectal_mucosal_cell_proliferation_in_humans_ L2 - http://cebp.aacrjournals.org/cgi/pmidlookup?view=long&amp;pmid=9752983 DB - PRIME DP - Unbound Medicine ER -