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Capillary electrophoresis of arsenic compounds with indirect fluorescence detection.
Electrophoresis. 1998 Sep; 19(12):2140-4.E

Abstract

A capillary electrophoresis (CE)-indirect fluorescence detection method for arsenic compounds is described. The five arsenic species, viz., arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), dimethylarsinate (DMA) and phenylarsonate (PhA), were efficiently separated by CE in 8 min with an 1.5 mM fluorescein solution at pH 9.8. Fluorescein also functioned as a background fluorophore for the indirect detection of these nonfluorescent arsenic species. Linearity (r> or =0.996) of more than two orders of magnitude was generally obtained. The relative standard deviation (RSD) values were in the ranges 0.4-0.7% and 2.2-8.2% for migration times and peak areas, respectively. The concentration limits of detection (CLODs) for the arsenic compounds studied were between 0.04 and 0.16 microg/mL (as arsenic). The detection sensitivity was generally dependent upon the transfer ratio (TR, defined as the number of moles of fluorescein ions displaced by one mole of analyte ions) of each arsenic species. The applicability of the method for the analysis of ground water was examined.

Authors+Show Affiliations

Huang YM
Department of Chemistry, Tunghai University, Taichung, Taiwan.
Whang CW
No affiliation info available

MeSH

ArsenicalsBuffersCalibrationElectrophoresis, CapillaryFluoresceinFluorometryHydrogen-Ion ConcentrationOsmosisQuality ControlSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9761195

Citation

Huang, Y M., and C W. Whang. "Capillary Electrophoresis of Arsenic Compounds With Indirect Fluorescence Detection." Electrophoresis, vol. 19, no. 12, 1998, pp. 2140-4.
Huang YM, Whang CW. Capillary electrophoresis of arsenic compounds with indirect fluorescence detection. Electrophoresis. 1998;19(12):2140-4.
Huang, Y. M., & Whang, C. W. (1998). Capillary electrophoresis of arsenic compounds with indirect fluorescence detection. Electrophoresis, 19(12), 2140-4.
Huang YM, Whang CW. Capillary Electrophoresis of Arsenic Compounds With Indirect Fluorescence Detection. Electrophoresis. 1998;19(12):2140-4. PubMed PMID: 9761195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis of arsenic compounds with indirect fluorescence detection. AU - Huang,Y M, AU - Whang,C W, PY - 1998/10/7/pubmed PY - 1998/10/7/medline PY - 1998/10/7/entrez SP - 2140 EP - 4 JF - Electrophoresis JO - Electrophoresis VL - 19 IS - 12 N2 - A capillary electrophoresis (CE)-indirect fluorescence detection method for arsenic compounds is described. The five arsenic species, viz., arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), dimethylarsinate (DMA) and phenylarsonate (PhA), were efficiently separated by CE in 8 min with an 1.5 mM fluorescein solution at pH 9.8. Fluorescein also functioned as a background fluorophore for the indirect detection of these nonfluorescent arsenic species. Linearity (r> or =0.996) of more than two orders of magnitude was generally obtained. The relative standard deviation (RSD) values were in the ranges 0.4-0.7% and 2.2-8.2% for migration times and peak areas, respectively. The concentration limits of detection (CLODs) for the arsenic compounds studied were between 0.04 and 0.16 microg/mL (as arsenic). The detection sensitivity was generally dependent upon the transfer ratio (TR, defined as the number of moles of fluorescein ions displaced by one mole of analyte ions) of each arsenic species. The applicability of the method for the analysis of ground water was examined. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9761195/Capillary_electrophoresis_of_arsenic_compounds_with_indirect_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.1150191217 DB - PRIME DP - Unbound Medicine ER -