Tags

Type your tag names separated by a space and hit enter

Nonaqueous capillary zone electrophoresis for separation of free fatty acids with indirect fluorescence detection.
Electrophoresis. 1998 Sep; 19(12):2187-92.E

Abstract

The feasibility of combining nonaqueous capillary zone electrophoresis with indirect fluorescence detection was studied for the efficient separation and sensitive detection of ionizable hydrophobic substances which do not possess practically suitable detection properties. Medium- and long-chain free fatty acids, C6-C24, were selected as test compounds. The results showed that such a wide range of medium- and long-chain free fatty acids could be separated between any two consecutive homologs in one run and be detected at a level of about 0.01-0.02 mM in highly basic methanol/acetonitrile media containing fluorescein as coion of background electrolyte for indirect fluorescence detection.

Authors+Show Affiliations

Wang T
Department of Chemistry, National University of Singapore, Republic of Singapore.
Wei H
No affiliation info available
Li SF
No affiliation info available

MeSH

AcetonitrilesElectrophoresis, CapillaryFatty Acids, NonesterifiedFluoresceinFluorescent DyesHydroxidesMethanolOsmosisPotassium Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9761202

Citation

Wang, T, et al. "Nonaqueous Capillary Zone Electrophoresis for Separation of Free Fatty Acids With Indirect Fluorescence Detection." Electrophoresis, vol. 19, no. 12, 1998, pp. 2187-92.
Wang T, Wei H, Li SF. Nonaqueous capillary zone electrophoresis for separation of free fatty acids with indirect fluorescence detection. Electrophoresis. 1998;19(12):2187-92.
Wang, T., Wei, H., & Li, S. F. (1998). Nonaqueous capillary zone electrophoresis for separation of free fatty acids with indirect fluorescence detection. Electrophoresis, 19(12), 2187-92.
Wang T, Wei H, Li SF. Nonaqueous Capillary Zone Electrophoresis for Separation of Free Fatty Acids With Indirect Fluorescence Detection. Electrophoresis. 1998;19(12):2187-92. PubMed PMID: 9761202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nonaqueous capillary zone electrophoresis for separation of free fatty acids with indirect fluorescence detection. AU - Wang,T, AU - Wei,H, AU - Li,S F, PY - 1998/10/7/pubmed PY - 1998/10/7/medline PY - 1998/10/7/entrez SP - 2187 EP - 92 JF - Electrophoresis JO - Electrophoresis VL - 19 IS - 12 N2 - The feasibility of combining nonaqueous capillary zone electrophoresis with indirect fluorescence detection was studied for the efficient separation and sensitive detection of ionizable hydrophobic substances which do not possess practically suitable detection properties. Medium- and long-chain free fatty acids, C6-C24, were selected as test compounds. The results showed that such a wide range of medium- and long-chain free fatty acids could be separated between any two consecutive homologs in one run and be detected at a level of about 0.01-0.02 mM in highly basic methanol/acetonitrile media containing fluorescein as coion of background electrolyte for indirect fluorescence detection. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9761202/Nonaqueous_capillary_zone_electrophoresis_for_separation_of_free_fatty_acids_with_indirect_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.1150191224 DB - PRIME DP - Unbound Medicine ER -