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Capillary electrophoresis/laser-induced fluorescence detection of fluorescein as a groundwater migration tracer.
Electrophoresis. 1998 Sep; 19(12):2252-6.E

Abstract

Capillary electrophoresis (CE) has been applied to the determination of the groundwater migration tracer dye fluorescein based on laser-induced fluorescence (LIF) detection and compared to determinations obtained with traditional spectrofluorimetry. Detection limits of injected dye in the low parts per trillion (ppt) ranges have been accomplished with both CE/LIF based on the Ar ion laser and with a spectrofluorimeter. This approach was used for a real-world problem in determining groundwater migration between adjacent Resource Conservation and Recovery Act (RCRA) and Superfund sites by the Environmental Sciences Division in response to regional needs and as application of new analytical tools under development. Fluorescent dye was injected into source wells and then was determined in monitoring wells by extracting pads that adsorbed the dye or by directly determining the dye in the water using solid-phase extraction (SPE), a preconcentration technique. The approaches based on CE/LIF exhibits increased specificity over existing approaches due to the separation and unique migration time of the dye. Additional studies were aimed at achieving sub-ppt levels in the water using solid-phase extraction and field-amplified injection techniques.

Authors+Show Affiliations

US Environmental Protection Agency, National Exposure Research Laboratory, Environmental Sciences Division, Las Vegas, NV 89193-3478, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

9761212

Citation

Ferguson, P L., et al. "Capillary Electrophoresis/laser-induced Fluorescence Detection of Fluorescein as a Groundwater Migration Tracer." Electrophoresis, vol. 19, no. 12, 1998, pp. 2252-6.
Ferguson PL, Grange AH, Brumley WC, et al. Capillary electrophoresis/laser-induced fluorescence detection of fluorescein as a groundwater migration tracer. Electrophoresis. 1998;19(12):2252-6.
Ferguson, P. L., Grange, A. H., Brumley, W. C., Donnelly, J. R., & Farley, J. W. (1998). Capillary electrophoresis/laser-induced fluorescence detection of fluorescein as a groundwater migration tracer. Electrophoresis, 19(12), 2252-6.
Ferguson PL, et al. Capillary Electrophoresis/laser-induced Fluorescence Detection of Fluorescein as a Groundwater Migration Tracer. Electrophoresis. 1998;19(12):2252-6. PubMed PMID: 9761212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis/laser-induced fluorescence detection of fluorescein as a groundwater migration tracer. AU - Ferguson,P L, AU - Grange,A H, AU - Brumley,W C, AU - Donnelly,J R, AU - Farley,J W, PY - 1998/10/7/pubmed PY - 1998/10/7/medline PY - 1998/10/7/entrez SP - 2252 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 19 IS - 12 N2 - Capillary electrophoresis (CE) has been applied to the determination of the groundwater migration tracer dye fluorescein based on laser-induced fluorescence (LIF) detection and compared to determinations obtained with traditional spectrofluorimetry. Detection limits of injected dye in the low parts per trillion (ppt) ranges have been accomplished with both CE/LIF based on the Ar ion laser and with a spectrofluorimeter. This approach was used for a real-world problem in determining groundwater migration between adjacent Resource Conservation and Recovery Act (RCRA) and Superfund sites by the Environmental Sciences Division in response to regional needs and as application of new analytical tools under development. Fluorescent dye was injected into source wells and then was determined in monitoring wells by extracting pads that adsorbed the dye or by directly determining the dye in the water using solid-phase extraction (SPE), a preconcentration technique. The approaches based on CE/LIF exhibits increased specificity over existing approaches due to the separation and unique migration time of the dye. Additional studies were aimed at achieving sub-ppt levels in the water using solid-phase extraction and field-amplified injection techniques. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9761212/Capillary_electrophoresis/laser_induced_fluorescence_detection_of_fluorescein_as_a_groundwater_migration_tracer_ DB - PRIME DP - Unbound Medicine ER -