The structure of serine hydroxymethyltransferase as modeled by homology and validated by site-directed mutagenesis.Protein Sci. 1998 Sep; 7(9):1976-82.PS
We describe a model for the three-dimensional structure of E. coli serine hydroxymethyltransferase based on its sequence homology with other PLP enzymes of the alpha-family and whose tertiary structures are known. The model suggests that certain amino acid residues at the putative active site of the enzyme can adopt specific roles in the catalytic mechanism. These proposals were supported by analysis of the properties of a number of site-directed mutants. New active site features are also proposed for further experimental testing.