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Leukocyte counts and macrophage phenotypes in induced sputum and bronchoalveolar lavage fluid from normal subjects.
Eur Respir J. 1998 Sep; 12(3):595-600.ER

Abstract

It is unclear whether leukocytes in induced sputum (IS) and bronchoalveolar lavage (BAL) represent the same cell populations. To compare leukocyte counts and macrophage phenotypes and investigate any measurable dithiothreitol (DTT)-mediated effect on macrophage immunocytochemical staining results, IS and BAL samples from nine healthy smokers and seven nonsmokers were examined. BAL and IS samples were processed and cell viability and cell counts were assessed. The macrophages were characterized by seven monoclonal antibodies (RFD1, RFD7, CD11b, CD54, CD68, CD71 and HLA-DR) using an indirect immunoalkaline phosphatase method. Intraindividual comparison of IS and BAL showed that IS samples from smokers and nonsmokers contained a lower total cell count (p<0.01 smokers, p<0.05 nonsmokers), a lower percentage of macrophages (both p<0.05) and a higher percentage of neutrophils (both p<0.05) than BAL samples. In addition, nonsmokers sputum samples contained a lower proportion of lymphocytes (p<0.05) than BAL. The macrophage expression of RFD7 and CD71 was higher in smokers sputum samples (both p<0.05) than in BAL, while nonsmokers sputum macrophages showed a higher expression of CD54 and CD71 (both p<0.05) than BAL macrophages. DTT-incubated BAL samples showed no difference in macrophage antigen expression from BAL samples not exposed to DTT. In conclusion, the relative proportions of leukocytes and the macrophage phenotypes differed between induced sputum and bronchoalveolar lavage suggesting that the methods provide samples from different lung compartments, inhabited by cells with different phenotypes.

Authors+Show Affiliations

Dept of Medicine, Karolinska Hospital, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9762786

Citation

Lensmar, C, et al. "Leukocyte Counts and Macrophage Phenotypes in Induced Sputum and Bronchoalveolar Lavage Fluid From Normal Subjects." The European Respiratory Journal, vol. 12, no. 3, 1998, pp. 595-600.
Lensmar C, Elmberger G, Sandgren P, et al. Leukocyte counts and macrophage phenotypes in induced sputum and bronchoalveolar lavage fluid from normal subjects. Eur Respir J. 1998;12(3):595-600.
Lensmar, C., Elmberger, G., Sandgren, P., Sköld, C. M., & Eklund, A. (1998). Leukocyte counts and macrophage phenotypes in induced sputum and bronchoalveolar lavage fluid from normal subjects. The European Respiratory Journal, 12(3), 595-600.
Lensmar C, et al. Leukocyte Counts and Macrophage Phenotypes in Induced Sputum and Bronchoalveolar Lavage Fluid From Normal Subjects. Eur Respir J. 1998;12(3):595-600. PubMed PMID: 9762786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukocyte counts and macrophage phenotypes in induced sputum and bronchoalveolar lavage fluid from normal subjects. AU - Lensmar,C, AU - Elmberger,G, AU - Sandgren,P, AU - Sköld,C M, AU - Eklund,A, PY - 1998/10/8/pubmed PY - 1998/10/8/medline PY - 1998/10/8/entrez SP - 595 EP - 600 JF - The European respiratory journal JO - Eur Respir J VL - 12 IS - 3 N2 - It is unclear whether leukocytes in induced sputum (IS) and bronchoalveolar lavage (BAL) represent the same cell populations. To compare leukocyte counts and macrophage phenotypes and investigate any measurable dithiothreitol (DTT)-mediated effect on macrophage immunocytochemical staining results, IS and BAL samples from nine healthy smokers and seven nonsmokers were examined. BAL and IS samples were processed and cell viability and cell counts were assessed. The macrophages were characterized by seven monoclonal antibodies (RFD1, RFD7, CD11b, CD54, CD68, CD71 and HLA-DR) using an indirect immunoalkaline phosphatase method. Intraindividual comparison of IS and BAL showed that IS samples from smokers and nonsmokers contained a lower total cell count (p<0.01 smokers, p<0.05 nonsmokers), a lower percentage of macrophages (both p<0.05) and a higher percentage of neutrophils (both p<0.05) than BAL samples. In addition, nonsmokers sputum samples contained a lower proportion of lymphocytes (p<0.05) than BAL. The macrophage expression of RFD7 and CD71 was higher in smokers sputum samples (both p<0.05) than in BAL, while nonsmokers sputum macrophages showed a higher expression of CD54 and CD71 (both p<0.05) than BAL macrophages. DTT-incubated BAL samples showed no difference in macrophage antigen expression from BAL samples not exposed to DTT. In conclusion, the relative proportions of leukocytes and the macrophage phenotypes differed between induced sputum and bronchoalveolar lavage suggesting that the methods provide samples from different lung compartments, inhabited by cells with different phenotypes. SN - 0903-1936 UR - https://www.unboundmedicine.com/medline/citation/9762786/Leukocyte_counts_and_macrophage_phenotypes_in_induced_sputum_and_bronchoalveolar_lavage_fluid_from_normal_subjects_ L2 - http://erj.ersjournals.com/cgi/pmidlookup?view=long&amp;pmid=9762786 DB - PRIME DP - Unbound Medicine ER -