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Effects of phytoestrogens on DNA synthesis in MCF-7 cells in the presence of estradiol or growth factors.
Nutr Cancer 1998; 31(2):90-100NC

Abstract

Phytoestrogen effects on estrogen action and tyrosine kinase activity have been proposed to contribute to cancer prevention. To study these mechanisms, a number of phytoestrogens and related compounds were evaluated for their effects on DNA synthesis (estimated by thymidine incorporation analysis) in estrogen-dependent MCF-7 cells in the presence of estradiol (E2), tamoxifen, insulin, or epidermal growth factor. We observed that 1) at 0.01-10 microM, genistein and coumestrol enhanced E2-induced DNA synthesis, as did 10 microM enterolactone. Chrysin at 1.0-10 microM and 10 microM luteolin or apigenin inhibited E2-induced DNA synthesis, as did all compounds at > 10 microM, 2) tamoxifen enhanced genistein-induced DNA synthesis but inhibited DNA synthesis induced by all other compounds, and 3) genistein enhanced insulin- and epidermal growth factor-induced DNA synthesis at 0.1-1.0 and 0.1-10 microM, respectively. At higher concentrations, inhibition was observed. Similar effects were seen with coumestrol. In conclusion, the effects of phytoestrogens in the presence of E2 or growth factors are concentration dependent and variable. At low concentrations, genistein and coumestrol significantly enhanced E2-induced and tyrosine kinase-mediated DNA synthesis; at high concentrations, inhibition was observed. Differing effects were observed with the other compounds. The variable effects of phytoestrogens on DNA synthesis must be considered when their roles in cancer prevention or treatment are evaluated.

Authors+Show Affiliations

Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9770719

Citation

Wang, C, and M S. Kurzer. "Effects of Phytoestrogens On DNA Synthesis in MCF-7 Cells in the Presence of Estradiol or Growth Factors." Nutrition and Cancer, vol. 31, no. 2, 1998, pp. 90-100.
Wang C, Kurzer MS. Effects of phytoestrogens on DNA synthesis in MCF-7 cells in the presence of estradiol or growth factors. Nutr Cancer. 1998;31(2):90-100.
Wang, C., & Kurzer, M. S. (1998). Effects of phytoestrogens on DNA synthesis in MCF-7 cells in the presence of estradiol or growth factors. Nutrition and Cancer, 31(2), pp. 90-100.
Wang C, Kurzer MS. Effects of Phytoestrogens On DNA Synthesis in MCF-7 Cells in the Presence of Estradiol or Growth Factors. Nutr Cancer. 1998;31(2):90-100. PubMed PMID: 9770719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of phytoestrogens on DNA synthesis in MCF-7 cells in the presence of estradiol or growth factors. AU - Wang,C, AU - Kurzer,M S, PY - 1998/10/15/pubmed PY - 1998/10/15/medline PY - 1998/10/15/entrez SP - 90 EP - 100 JF - Nutrition and cancer JO - Nutr Cancer VL - 31 IS - 2 N2 - Phytoestrogen effects on estrogen action and tyrosine kinase activity have been proposed to contribute to cancer prevention. To study these mechanisms, a number of phytoestrogens and related compounds were evaluated for their effects on DNA synthesis (estimated by thymidine incorporation analysis) in estrogen-dependent MCF-7 cells in the presence of estradiol (E2), tamoxifen, insulin, or epidermal growth factor. We observed that 1) at 0.01-10 microM, genistein and coumestrol enhanced E2-induced DNA synthesis, as did 10 microM enterolactone. Chrysin at 1.0-10 microM and 10 microM luteolin or apigenin inhibited E2-induced DNA synthesis, as did all compounds at > 10 microM, 2) tamoxifen enhanced genistein-induced DNA synthesis but inhibited DNA synthesis induced by all other compounds, and 3) genistein enhanced insulin- and epidermal growth factor-induced DNA synthesis at 0.1-1.0 and 0.1-10 microM, respectively. At higher concentrations, inhibition was observed. Similar effects were seen with coumestrol. In conclusion, the effects of phytoestrogens in the presence of E2 or growth factors are concentration dependent and variable. At low concentrations, genistein and coumestrol significantly enhanced E2-induced and tyrosine kinase-mediated DNA synthesis; at high concentrations, inhibition was observed. Differing effects were observed with the other compounds. The variable effects of phytoestrogens on DNA synthesis must be considered when their roles in cancer prevention or treatment are evaluated. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/9770719/Effects_of_phytoestrogens_on_DNA_synthesis_in_MCF_7_cells_in_the_presence_of_estradiol_or_growth_factors_ L2 - http://www.tandfonline.com/doi/full/10.1080/01635589809514686 DB - PRIME DP - Unbound Medicine ER -