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Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 2.FEBS Lett. 1998 Sep 25; 436(1):111-4.FL
Abstract
Hepatocyte growth factor activator inhibitor type 2 (HAI-2) was identified as a potent inhibitor of hepatocyte growth factor activator (HGF activator). The primary translation product of HAI-2 contains two Kunitz domains. To characterize their function, we introduced a point mutation into the reactive site of each Kunitz domain, and assayed the mutants for their HGF activator inhibitory activity. A point mutation in the COOH-terminal Kunitz domain did not affect the activity of HAI-2, whereas a point mutation in the NH2-terminal Kunitz domain markedly reduced the activity. These results suggest that the NH2-terminal Kunitz domain is mainly responsible for the HGF activator inhibitory activity of HAI-2.
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MeSH
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
9771903
Citation
Qin, L, et al. "Functional Characterization of Kunitz Domains in Hepatocyte Growth Factor Activator Inhibitor Type 2." FEBS Letters, vol. 436, no. 1, 1998, pp. 111-4.
Qin L, Denda K, Shimomura T, et al. Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 2. FEBS Lett. 1998;436(1):111-4.
Qin, L., Denda, K., Shimomura, T., Kawaguchi, T., & Kitamura, N. (1998). Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 2. FEBS Letters, 436(1), 111-4.
Qin L, et al. Functional Characterization of Kunitz Domains in Hepatocyte Growth Factor Activator Inhibitor Type 2. FEBS Lett. 1998 Sep 25;436(1):111-4. PubMed PMID: 9771903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 2.
AU - Qin,L,
AU - Denda,K,
AU - Shimomura,T,
AU - Kawaguchi,T,
AU - Kitamura,N,
PY - 1998/10/15/pubmed
PY - 1998/10/15/medline
PY - 1998/10/15/entrez
SP - 111
EP - 4
JF - FEBS letters
JO - FEBS Lett
VL - 436
IS - 1
N2 - Hepatocyte growth factor activator inhibitor type 2 (HAI-2) was identified as a potent inhibitor of hepatocyte growth factor activator (HGF activator). The primary translation product of HAI-2 contains two Kunitz domains. To characterize their function, we introduced a point mutation into the reactive site of each Kunitz domain, and assayed the mutants for their HGF activator inhibitory activity. A point mutation in the COOH-terminal Kunitz domain did not affect the activity of HAI-2, whereas a point mutation in the NH2-terminal Kunitz domain markedly reduced the activity. These results suggest that the NH2-terminal Kunitz domain is mainly responsible for the HGF activator inhibitory activity of HAI-2.
SN - 0014-5793
UR - https://www.unboundmedicine.com/medline/citation/9771903/Functional_characterization_of_Kunitz_domains_in_hepatocyte_growth_factor_activator_inhibitor_type_2_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(98)01105-3
DB - PRIME
DP - Unbound Medicine
ER -
