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The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein.
EMBO J. 1998 Oct 15; 17(20):6061-8.EJ

Abstract

The OxyS regulatory RNA integrates the adaptive response to hydrogen peroxide with other cellular stress responses and protects against DNA damage. Among the OxyS targets is the rpoS-encoded sigma(s) subunit of RNA polymerase. Sigma(s) is a central regulator of genes induced by osmotic stress, starvation and entry into stationary phase. We examined the mechanism whereby OxyS represses rpoS expression and found that the OxyS RNA inhibits translation of the rpoS message. This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I). Co-immunoprecipitation and gel mobility shift experiments revealed that the OxyS RNA binds Hfq, suggesting that OxyS represses rpoS translation by altering Hfq activity.

Authors+Show Affiliations

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9774349

Citation

Zhang, A, et al. "The OxyS Regulatory RNA Represses rpoS Translation and Binds the Hfq (HF-I) Protein." The EMBO Journal, vol. 17, no. 20, 1998, pp. 6061-8.
Zhang A, Altuvia S, Tiwari A, et al. The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein. EMBO J. 1998;17(20):6061-8.
Zhang, A., Altuvia, S., Tiwari, A., Argaman, L., Hengge-Aronis, R., & Storz, G. (1998). The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein. The EMBO Journal, 17(20), 6061-8.
Zhang A, et al. The OxyS Regulatory RNA Represses rpoS Translation and Binds the Hfq (HF-I) Protein. EMBO J. 1998 Oct 15;17(20):6061-8. PubMed PMID: 9774349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein. AU - Zhang,A, AU - Altuvia,S, AU - Tiwari,A, AU - Argaman,L, AU - Hengge-Aronis,R, AU - Storz,G, PY - 1998/10/17/pubmed PY - 1998/10/17/medline PY - 1998/10/17/entrez SP - 6061 EP - 8 JF - The EMBO journal JO - EMBO J VL - 17 IS - 20 N2 - The OxyS regulatory RNA integrates the adaptive response to hydrogen peroxide with other cellular stress responses and protects against DNA damage. Among the OxyS targets is the rpoS-encoded sigma(s) subunit of RNA polymerase. Sigma(s) is a central regulator of genes induced by osmotic stress, starvation and entry into stationary phase. We examined the mechanism whereby OxyS represses rpoS expression and found that the OxyS RNA inhibits translation of the rpoS message. This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I). Co-immunoprecipitation and gel mobility shift experiments revealed that the OxyS RNA binds Hfq, suggesting that OxyS represses rpoS translation by altering Hfq activity. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/9774349/The_OxyS_regulatory_RNA_represses_rpoS_translation_and_binds_the_Hfq__HF_I__protein_ L2 - https://doi.org/10.1093/emboj/17.20.6061 DB - PRIME DP - Unbound Medicine ER -